ID   ESXT_MYCTU              Reviewed;         100 AA.
AC   I6YC53;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=ESAT-6-like protein EsxT {ECO:0000305};
GN   Name=esxT {ECO:0000303|PubMed:19876390};
GN   OrderedLocusNames=Rv3444c {ECO:0000312|EMBL:CCP46266.1};
GN   ORFNames=LH57_18810 {ECO:0000312|EMBL:AIR16239.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4]
RP   SUBUNIT.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20085764; DOI=10.1016/j.febslet.2009.12.057;
RA   Poulsen C., Holton S., Geerlof A., Wilmanns M., Song Y.H.;
RT   "Stoichiometric protein complex formation and over-expression using the
RT   prokaryotic native operon structure.";
RL   FEBS Lett. 584:669-674(2010).
RN   [5]
RP   FUNCTION IN VIRULENCE.
RC   STRAIN=H37Rv;
RX   PubMed=25242740; DOI=10.1016/j.bbrc.2014.09.047;
RA   Shi J., Zhang H., Fang L., Xi Y., Zhou Y., Luo R., Wang D., Xiao S.,
RA   Chen H.;
RT   "A novel firefly luciferase biosensor enhances the detection of apoptosis
RT   induced by ESAT-6 family proteins of Mycobacterium tuberculosis.";
RL   Biochem. Biophys. Res. Commun. 452:1046-1053(2014).
RN   [6]
RP   SUBUNIT, INTERACTION WITH ESXU, DEVELOPMENTAL STAGE, AND HUMORAL RESPONSE.
RC   STRAIN=H37Rv;
RX   PubMed=29559126; DOI=10.1016/j.tube.2018.02.002;
RA   Pandey H., Fatma F., Yabaji S.M., Kumari M., Tripathi S., Srivastava K.,
RA   Tripathi D.K., Kant S., Srivastava K.K., Arora A.;
RT   "Biophysical and immunological characterization of the ESX-4 system ESAT-6
RT   family proteins Rv3444c and Rv3445c from Mycobacterium tuberculosis
RT   H37Rv.";
RL   Tuberculosis 109:85-96(2018).
CC   -!- FUNCTION: Induces apoptosis of host cells via activation of NF-kappa-B.
CC       {ECO:0000269|PubMed:25242740}.
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxU (PubMed:20085764,
CC       PubMed:29559126). Complex formation results in induction of alpha-
CC       helical conformation and stability against chemical denaturation
CC       (PubMed:29559126). {ECO:0000269|PubMed:20085764,
CC       ECO:0000269|PubMed:29559126}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}.
CC       Note=Probably secreted via the ESX-4 / type VII secretion system
CC       (T7SS). {ECO:0000305|PubMed:19876390}.
CC   -!- DEVELOPMENTAL STAGE: Actively produced during the active phase of
CC       tuberculosis. {ECO:0000269|PubMed:29559126}.
CC   -!- MISCELLANEOUS: Elicits strong humoral responses. Induces significant
CC       lymphocyte proliferation and up-regulates the induction of TNF-alpha
CC       and IL-6 in tuberculosis patients. {ECO:0000269|PubMed:29559126}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; CP009480; AIR16239.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP46266.1; -; Genomic_DNA.
DR   RefSeq; NP_217961.1; NC_000962.3.
DR   RefSeq; WP_003900059.1; NZ_NVQJ01000065.1.
DR   AlphaFoldDB; I6YC53; -.
DR   SMR; I6YC53; -.
DR   STRING; 83332.Rv3444c; -.
DR   PaxDb; I6YC53; -.
DR   DNASU; 887587; -.
DR   GeneID; 887587; -.
DR   KEGG; mtu:Rv3444c; -.
DR   PATRIC; fig|83332.111.peg.3839; -.
DR   TubercuList; Rv3444c; -.
DR   eggNOG; COG4842; Bacteria.
DR   HOGENOM; CLU_151185_3_4_11; -.
DR   OMA; PLQQTWT; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..100
FT                   /note="ESAT-6-like protein EsxT"
FT                   /id="PRO_0000436933"
SQ   SEQUENCE   100 AA;  11120 MW;  5046B1B46578DC6C CRC64;
     MNADPVLSYN FDAIEYSVRQ EIHTTAARFN AALQELRSQI APLQQLWTRE AAAAYHAEQL
     KWHQAASALN EILIDLGNAV RHGADDVAHA DRRAAGAWAR