ID   ESXU_MYCTU              Reviewed;         105 AA.
AC   I6Y3I6; L0TE56;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   19-FEB-2014, sequence version 2.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=ESAT-6-like protein EsxU {ECO:0000305};
GN   Name=esxU {ECO:0000303|PubMed:19876390};
GN   OrderedLocusNames=Rv3445c {ECO:0000312|EMBL:CCP46267.1};
GN   ORFNames=LH57_18815 {ECO:0000312|EMBL:AIR16240.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4]
RP   SUBUNIT.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20085764; DOI=10.1016/j.febslet.2009.12.057;
RA   Poulsen C., Holton S., Geerlof A., Wilmanns M., Song Y.H.;
RT   "Stoichiometric protein complex formation and over-expression using the
RT   prokaryotic native operon structure.";
RL   FEBS Lett. 584:669-674(2010).
RN   [5]
RP   SUBUNIT, INTERACTION WITH ESXT, DEVELOPMENTAL STAGE, AND HUMORAL RESPONSE.
RC   STRAIN=H37Rv;
RX   PubMed=29559126; DOI=10.1016/j.tube.2018.02.002;
RA   Pandey H., Fatma F., Yabaji S.M., Kumari M., Tripathi S., Srivastava K.,
RA   Tripathi D.K., Kant S., Srivastava K.K., Arora A.;
RT   "Biophysical and immunological characterization of the ESX-4 system ESAT-6
RT   family proteins Rv3444c and Rv3445c from Mycobacterium tuberculosis
RT   H37Rv.";
RL   Tuberculosis 109:85-96(2018).
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxT (PubMed:20085764,
CC       PubMed:29559126). Complex formation results in induction of alpha-
CC       helical conformation and stability against chemical denaturation
CC       (PubMed:29559126). {ECO:0000269|PubMed:20085764,
CC       ECO:0000269|PubMed:29559126}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}.
CC       Note=Probably secreted via the ESX-4 / type VII secretion system
CC       (T7SS). {ECO:0000305|PubMed:19876390}.
CC   -!- DEVELOPMENTAL STAGE: Actively produced during the active phase of
CC       tuberculosis. {ECO:0000269|PubMed:29559126}.
CC   -!- MISCELLANEOUS: Elicits strong humoral responses. Induces significant
CC       lymphocyte proliferation and up-regulates the induction of TNF-alpha
CC       and IL-6 in tuberculosis patients. {ECO:0000269|PubMed:29559126}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. CFP-10 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; CP009480; AIR16240.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP46267.1; -; Genomic_DNA.
DR   RefSeq; NP_217962.2; NC_000962.3.
DR   RefSeq; WP_003900060.1; NZ_NVQJ01000065.1.
DR   AlphaFoldDB; I6Y3I6; -.
DR   SMR; I6Y3I6; -.
DR   STRING; 83332.Rv3445c; -.
DR   PaxDb; I6Y3I6; -.
DR   GeneID; 45427435; -.
DR   GeneID; 887585; -.
DR   KEGG; mtu:Rv3445c; -.
DR   PATRIC; fig|83332.111.peg.3840; -.
DR   TubercuList; Rv3445c; -.
DR   eggNOG; COG4842; Bacteria.
DR   HOGENOM; CLU_165460_0_0_11; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..105
FT                   /note="ESAT-6-like protein EsxU"
FT                   /id="PRO_0000436935"
SQ   SEQUENCE   105 AA;  11434 MW;  EBE82C2538F37092 CRC64;
     MSTPNTLNAD FDLMRSVAGI TDARNEEIRA MLQAFIGRMS GVPPSVWGGL AAARFQDVVD
     RWNAESTRLY HVLHAIADTI RHNEAALREA GQIHARHIAA AGGDL