ID   ESXV_MYCTU              Reviewed;          94 AA.
AC   P0DOA7; L0TEQ6; O08120; O08122; P96364; P9WNK1; Q9L781;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   07-SEP-2016, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=ESAT-6-like protein EsxV {ECO:0000305};
DE   AltName: Full=Antigen Mtb9.9B;
GN   Name=esxV {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv3619c;
GN   ORFNames=MTCY07H7B.03, MTCY15C10.33;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=10662800; DOI=10.1084/jem.191.3.551;
RA   Alderson M.R., Bement T., Day C.H., Zhu L., Molesh D., Skeiky Y.A.W.,
RA   Coler R., Lewinsohn D.M., Reed S.G., Dillon D.C.;
RT   "Expression cloning of an immunodominant family of Mycobacterium
RT   tuberculosis antigens using human CD4(+) T cells.";
RL   J. Exp. Med. 191:551-560(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4]
RP   FUNCTION IN VIRULENCE, INTERACTION WITH ESXW, AND SUBCELLULAR LOCATION.
RC   STRAIN=H37Rv;
RX   PubMed=21134129; DOI=10.1111/j.1742-4658.2010.07958.x;
RA   Mahmood A., Srivastava S., Tripathi S., Ansari M.A., Owais M., Arora A.;
RT   "Molecular characterization of secretory proteins Rv3619c and Rv3620c from
RT   Mycobacterium tuberculosis H37Rv.";
RL   FEBS J. 278:341-353(2011).
CC   -!- FUNCTION: Probable virulence factor. In mice, elicits increased levels
CC       of IFN-gamma, IL-12 and IgG(2a), indicating a dominant Th1 host immune
CC       response. {ECO:0000269|PubMed:21134129}.
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxW. The complex is
CC       destabilized at low pH. Unfolding of the proteins is required for
CC       dissociation of the complex and membrane binding.
CC       {ECO:0000269|PubMed:21134129}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}.
CC       Note=Probably secreted via the ESX-5 / type VII secretion system (T7SS)
CC       (Probable). May bind to lipid membranes after dissociation of the
CC       complex (Probable). {ECO:0000305|PubMed:19876390,
CC       ECO:0000305|PubMed:21134129}.
CC   -!- MISCELLANEOUS: Potent T-cell antigen that could be a subunit vaccine
CC       candidate. {ECO:0000305|PubMed:21134129}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; AF226277; AAF32406.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP46442.1; -; Genomic_DNA.
DR   PIR; D70560; D70560.
DR   RefSeq; NP_215553.1; NC_000962.3.
DR   RefSeq; NP_218136.1; NC_000962.3.
DR   RefSeq; WP_003898699.1; NZ_NVQJ01000149.1.
DR   AlphaFoldDB; P0DOA7; -.
DR   SMR; P0DOA7; -.
DR   DNASU; 888299; -.
DR   GeneID; 885328; -.
DR   GeneID; 888299; -.
DR   KEGG; mtu:Rv1037c; -.
DR   KEGG; mtu:Rv3619c; -.
DR   TubercuList; Rv3619c; -.
DR   PhylomeDB; P0DOA7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; IDA:UniProtKB.
DR   InterPro; IPR009416; ESAT-6-like_Myco.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   PIRSF; PIRSF037656; DUF1066; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..94
FT                   /note="ESAT-6-like protein EsxV"
FT                   /id="PRO_0000436928"
FT   CONFLICT        20
FT                   /note="Q -> L (in Ref. 1; AAF32406)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        23
FT                   /note="S -> L (in Ref. 1; AAF32406)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   94 AA;  9833 MW;  8F971BB03789C57E CRC64;
     MTINYQFGDV DAHGAMIRAQ AGSLEAEHQA IISDVLTASD FWGGAGSAAC QGFITQLGRN
     FQVIYEQANA HGQKVQAAGN NMAQTDSAVG SSWA