ET2_ARATH
ID ET2_ARATH Reviewed; 483 AA.
AC F4K933; Q9FJT5;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Protein EFFECTOR OF TRANSCRIPTION 2 {ECO:0000303|PubMed:17991462};
DE Short=AtET2 {ECO:0000303|PubMed:17991462};
GN Name=ET2 {ECO:0000303|PubMed:17991462};
GN OrderedLocusNames=At5g56780 {ECO:0000312|Araport:AT5G56780};
GN ORFNames=MIK19.25 {ECO:0000312|EMBL:BAB09896.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GIY-YIG DOMAIN, AND
RP MUTAGENESIS OF ARG-103.
RX PubMed=17991462; DOI=10.1016/j.ydbio.2007.09.061;
RA Ivanov R., Tiedemann J., Czihal A., Schallau A., Diep L.H., Mock H.P.,
RA Claus B., Tewes A., Baeumlein H.;
RT "EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and
RT includes a functional DNA single strand cutting domain.";
RL Dev. Biol. 313:93-106(2008).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=22226340; DOI=10.1016/j.jplph.2011.11.017;
RA Ivanov R., Tiedemann J., Czihal A., Baumlein H.;
RT "Transcriptional regulator AtET2 is required for the induction of dormancy
RT during late seed development.";
RL J. Plant Physiol. 169:501-508(2012).
CC -!- FUNCTION: Transcriptional regulator involved in the regulation of cell
CC differentiation in meristems. Probably regulates the expression of
CC various KNAT genes involved in the maintenance of the cells in an
CC undifferentiated, merismastic state. Plays a role in the regulation of
CC gibberellin 20 oxidase and the gibberellin-regulated protein GASA4.
CC Localizes in the nucleus during the cellular differentiation state and
CC may act via a single strand cutting domain (PubMed:17991462).
CC Transcriptional regulator required for the induction of dormancy during
CC late seed development. Interacts genetically with FUS3 and may be
CC component of the same regulatory pathway during embryogenesis. Binds
CC both linear and supercoiled DNA without sequence preference
CC (PubMed:22226340). {ECO:0000269|PubMed:17991462,
CC ECO:0000269|PubMed:22226340}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17991462}. Nucleus
CC {ECO:0000269|PubMed:17991462}. Note=Localizes to the cytoplasm in non-
CC differentiating cells and to the nucleus in differentiating cells.
CC {ECO:0000269|PubMed:17991462}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues of stems, hypocotyls,
CC leaves and flowers. Expressed in the vascular bundles of xylem in shoot
CC parenchyma cells. Expressed in the remnant cytoplasm of differentiated
CC fiber cells and in protoxylem element of parenchymal cells.
CC {ECO:0000269|PubMed:17991462}.
CC -!- DEVELOPMENTAL STAGE: During embryogenesis, expressed in the embryo at
CC the late torpedo/cotyledon stage, at the onset of maturation. Not
CC expressed during early stages of seed development or in mature seeds.
CC {ECO:0000269|PubMed:22226340}.
CC -!- DOMAIN: Contains a bacterial GIY-YIG-like domain which can functionally
CC replace the bacterial GIY-YIG domain in the UVRC protein.
CC {ECO:0000305|PubMed:17991462}.
CC -!- DISRUPTION PHENOTYPE: Reduced levels of lignin in leaves and stems
CC (PubMed:17991462). Reduced seed germination rate (PubMed:22226340).
CC {ECO:0000269|PubMed:17991462, ECO:0000269|PubMed:22226340}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09896.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB013392; BAB09896.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96806.1; -; Genomic_DNA.
DR RefSeq; NP_200489.2; NM_125061.4.
DR AlphaFoldDB; F4K933; -.
DR IntAct; F4K933; 3.
DR STRING; 3702.AT5G56780.1; -.
DR PaxDb; F4K933; -.
DR PRIDE; F4K933; -.
DR EnsemblPlants; AT5G56780.1; AT5G56780.1; AT5G56780.
DR GeneID; 835780; -.
DR Gramene; AT5G56780.1; AT5G56780.1; AT5G56780.
DR KEGG; ath:AT5G56780; -.
DR Araport; AT5G56780; -.
DR TAIR; locus:2165026; AT5G56780.
DR eggNOG; ENOG502QXNF; Eukaryota.
DR HOGENOM; CLU_025361_0_0_1; -.
DR InParanoid; F4K933; -.
DR OMA; ESFASIC; -.
DR OrthoDB; 431818at2759; -.
DR PRO; PR:F4K933; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K933; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0097100; F:supercoiled DNA binding; IDA:TAIR.
DR GO; GO:0006306; P:DNA methylation; IGI:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:2000033; P:regulation of seed dormancy process; IMP:TAIR.
DR GO; GO:0010089; P:xylem development; IMP:UniProtKB.
DR InterPro; IPR038909; Effector_transcript.
DR PANTHER; PTHR35133; PTHR35133; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..483
FT /note="Protein EFFECTOR OF TRANSCRIPTION 2"
FT /id="PRO_0000436019"
FT DOMAIN 64..112
FT /note="GIY-YIG"
FT /evidence="ECO:0000305|PubMed:17991462"
FT REPEAT 278..303
FT /note="Cx9Cx9RCx2HK"
FT /evidence="ECO:0000305|PubMed:17991462"
FT REPEAT 338..363
FT /note="Cx9Cx9RCx2HK"
FT /evidence="ECO:0000305|PubMed:17991462"
FT REPEAT 406..431
FT /note="Cx9Cx9RCx2HK"
FT /evidence="ECO:0000305|PubMed:17991462"
FT REPEAT 453..478
FT /note="Cx9Cx9RCx2HK"
FT /evidence="ECO:0000305|PubMed:17991462"
FT REGION 380..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 103
FT /note="R->A: Defective in GIY-YIG domain."
FT /evidence="ECO:0000269|PubMed:17991462"
SQ SEQUENCE 483 AA; 54589 MW; 66B391A32E35C009 CRC64;
MEFGDGVSFA VVPTVFKRED YKRTKHDTVF SKWQVLIGSN DWEDFKNGKD GVGRYRVQNL
PRKSCPGLYE LGVAVIGQEQ CRKLEPDIVL ASYLGQAESV RSRLQRYGRS GAHLRNVNNL
NDCETIESPV KAVTGGLFED IFSKGGSILY RWAPMGSKRE AEATEGMLLS TFDYAWNKGS
NGERRQLDLL KKLGDREFMS KRKSGISRML FPFLRNQVGI RIKGEKHVLK EERKLTCDVD
EEKSNNFLTS ILKLTRSRPQ PVSDRFDEVD GSCSDIVCGV LLEDGGCCIR SPVKGRKRCI
EHKGQRVCRV SPEKQTPPKS EIFTGQDHHN HKDSDVVCGV ILPDMEPCNK RPVPGRKRCE
DHKGMRINAF LFLLNQTDRE KTVKDEKPDP ESHTESIEEE ALTRFCEATT KNGLPCTRSS
PKGSKRCWQH KEKTSSDTSP VYFQPEAAKN VACGVKLGNG LICERSPVKG RKRCEEHKGM
RIT
