ID   ETCHF_ENTFC             Reviewed;          58 AA.
AC   P86183; X5D0R7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   09-JUL-2014, sequence version 2.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Enterocin-HF;
DE   Flags: Precursor;
GN   Name=entHF;
OS   Enterococcus faecium (Streptococcus faecium).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1352;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HS38;
RA   Campanero C., Brandao A., Almeida T., Igrejas G., Poeta P., Hernandez P.E.,
RA   Herranz C., Cintas L.M.;
RT   "Enterocin HF, a new pediocin-like bacteriocin produced by Enterococcus
RT   faecium strains of human and fish origin.";
RL   Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 16-58, FUNCTION, AND MASS SPECTROMETRY.
RC   STRAIN=HS, and TA29;
RA   Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E.,
RA   Poeta P., Cintas L.M., Herranz C.;
RT   "Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin
RT   produced by E. faecium HS and E. faecium TA29 isolated from humans and fish
RT   (thin-lipped grey mullet; Liza ramada) in Portugal.";
RL   Submitted (JAN-2009) to UniProtKB.
CC   -!- FUNCTION: Bacteriocin. {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=4332; Mass_error=1; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.2};
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
CC       {ECO:0000255}.
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DR   EMBL; KJ442693; AHW46186.1; -; Genomic_DNA.
DR   RefSeq; WP_048340600.1; NZ_LAXK01000018.1.
DR   PDB; 2N4K; NMR; -; A=16-58.
DR   PDBsum; 2N4K; -.
DR   AlphaFoldDB; P86183; -.
DR   SMR; P86183; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.5.130; -; 1.
DR   InterPro; IPR002633; Bacteriocin_IIa.
DR   InterPro; IPR023384; Bacteriocin_IIa_CS.
DR   InterPro; IPR023388; Bacteriocin_IIa_dom_sf.
DR   Pfam; PF01721; Bacteriocin_II; 1.
DR   PROSITE; PS60030; BACTERIOCIN_IIA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PROPEP          1..15
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000429796"
FT   CHAIN           16..58
FT                   /note="Enterocin-HF"
FT                   /id="PRO_0000371459"
FT   DISULFID        24..29
FT                   /evidence="ECO:0000250"
FT   CONFLICT        55..58
FT                   /note="GWKG -> AWAC (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   STRAND          19..21
FT                   /evidence="ECO:0007829|PDB:2N4K"
FT   STRAND          24..27
FT                   /evidence="ECO:0007829|PDB:2N4K"
FT   HELIX           36..49
FT                   /evidence="ECO:0007829|PDB:2N4K"
SQ   SEQUENCE   58 AA;  5949 MW;  A5A312BDA092DBA0 CRC64;
     MEKLTVKEMS QVVGGKYYGN GVSCNKKGCS VDWGKAIGII GNNAAANLTT GGKAGWKG