ID   ETCP_ENTFC              Reviewed;          71 AA.
AC   O30434; Q0GFE0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Bacteriocin enterocin-P;
DE   Flags: Precursor;
GN   Name=entP {ECO:0000312|EMBL:AAC45870.1};
OS   Enterococcus faecium (Streptococcus faecium).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1352;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAC45870.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-70, FUNCTION, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=P13 {ECO:0000312|EMBL:AAC45870.1};
RX   PubMed=9361419; DOI=10.1128/aem.63.11.4321-4330.1997;
RA   Cintas L.M., Casaus P., Haavarstein L.S., Hernandez P.E., Nes I.F.;
RT   "Biochemical and genetic characterization of enterocin P, a novel sec-
RT   dependent bacteriocin from Enterococcus faecium P13 with a broad
RT   antimicrobial spectrum.";
RL   Appl. Environ. Microbiol. 63:4321-4330(1997).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:ABI29857.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-71, FUNCTION, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=LHICA 28-4 {ECO:0000312|EMBL:ABI29856.1}, and
RC   LHICA 51 {ECO:0000312|EMBL:ABI29857.1};
RX   PubMed=17103378; DOI=10.1002/mnfr.200600178;
RA   Arlindo S., Calo P., Franco C., Prado M., Cepeda A., Barros-Velazquez J.;
RT   "Single nucleotide polymorphism analysis of the enterocin P structural gene
RT   of Enterococcus faecium strains isolated from nonfermented animal foods.";
RL   Mol. Nutr. Food Res. 50:1229-1238(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-71.
RC   STRAIN=LHICA 34-5, and LHICA 40-4;
RA   Hosseini S.V., Arlindo S., Fernandez-No C., Calo-Mata P.,
RA   Barros-Velazquez J.;
RT   "Characterization of the probiotic profile of bacteriocin-producing
RT   Enterococcus faecium strains isolated from animal foods.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000305}
RP   MASS SPECTROMETRY.
RX   PubMed=17551760; DOI=10.1007/s10295-007-0226-4;
RA   Marekova M., Laukova A., Skaugen M., Nes I.;
RT   "Isolation and characterization of a new bacteriocin, termed enterocin M,
RT   produced by environmental isolate Enterococcus faecium AL41.";
RL   J. Ind. Microbiol. Biotechnol. 34:533-537(2007).
CC   -!- FUNCTION: Bacteriocin that inhibits the growth of wide range of Gram-
CC       positive bacterial species. Does not inhibit the growth of several
CC       strains of Gram-negative bacteria. {ECO:0000269|PubMed:17103378,
CC       ECO:0000269|PubMed:9361419}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Stable between pH 3.0 and pH 7.0. {ECO:0000269|PubMed:17103378};
CC       Temperature dependence:
CC         Thermostable, retains activity after heating at 100 and 121 degrees
CC         Celsius. {ECO:0000269|PubMed:17103378};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9361419}.
CC   -!- MASS SPECTROMETRY: Mass=4701.25; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17551760};
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIA/YGNGV family.
CC       {ECO:0000255}.
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DR   EMBL; AF005726; AAC45870.1; -; Genomic_DNA.
DR   EMBL; DQ867124; ABI29856.1; -; Genomic_DNA.
DR   EMBL; DQ867125; ABI29857.1; -; Genomic_DNA.
DR   EMBL; FJ416486; ACJ46052.1; -; Genomic_DNA.
DR   EMBL; FJ416487; ACJ46053.1; -; Genomic_DNA.
DR   AlphaFoldDB; O30434; -.
DR   SMR; O30434; -.
DR   TCDB; 1.C.24.1.10; the pediocin (pediocin) family.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   Gene3D; 1.20.5.130; -; 1.
DR   InterPro; IPR002633; Bacteriocin_IIa.
DR   InterPro; IPR023384; Bacteriocin_IIa_CS.
DR   InterPro; IPR023388; Bacteriocin_IIa_dom_sf.
DR   Pfam; PF01721; Bacteriocin_II; 1.
DR   PROSITE; PS60030; BACTERIOCIN_IIA; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:9361419"
FT   CHAIN           28..71
FT                   /note="Bacteriocin enterocin-P"
FT                   /id="PRO_5000053228"
FT   DISULFID        38..43
FT                   /evidence="ECO:0000250|UniProtKB:P83002"
SQ   SEQUENCE   71 AA;  7552 MW;  8CAC2BF6EEF59978 CRC64;
     MRKKLFSLAL IGIFGLVVTN FGTKVDAATR SYGNGVYCNN SKCWVNWGEA KENIAGIVIS
     GWASGLAGMG H