ID   ETF1_VARV               Reviewed;         637 AA.
AC   P0DOO0; P33056; Q9QNI6;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Early transcription factor 70 kDa subunit;
DE            EC=3.6.4.-;
DE   AltName: Full=ATP-dependent helicase VETFS;
DE   AltName: Full=ETF small subunit;
DE   AltName: Full=VETF D6 subunit;
GN   Name=VETFS; ORFNames=D6R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RX   PubMed=10639322; DOI=10.1006/viro.1999.0086;
RA   Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V.,
RA   Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J.;
RT   "Alastrim smallpox variola minor virus genome DNA sequences.";
RL   Virology 266:361-386(2000).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from early
CC       gene promoters. Is recruited by the RPO-associated protein of 94 kDa
CC       (RAP94) to form the early transcription complex, which also contains
CC       the core RNA polymerase. ETF heterodimer binds to early gene promoters
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a 70 kDa and a 82 kDa subunit. Part of the
CC       early transcription complex composed of ETF, RAP94, and the DNA-
CC       directed RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- SIMILARITY: Belongs to the helicase family. VETF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60844.1; -; Genomic_DNA.
DR   EMBL; Y16780; CAB54696.1; -; Genomic_DNA.
DR   PIR; F72162; F72162.
DR   PIR; T28534; T28534.
DR   SMR; P0DOO0; -.
DR   Proteomes; UP000111493; Genome.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Transcription; Transcription regulation; Virion.
FT   CHAIN           1..637
FT                   /note="Early transcription factor 70 kDa subunit"
FT                   /id="PRO_0000448131"
FT   DOMAIN          32..185
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          327..507
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           135..138
FT                   /note="DEXH box"
FT   BINDING         45..52
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   VARIANT         136
FT                   /note="K -> E (in strain: Garcia-1966)"
FT   VARIANT         462
FT                   /note="V -> A (in strain: Garcia-1966)"
SQ   SEQUENCE   637 AA;  73830 MW;  FA1E882BCF0CDFD7 CRC64;
     MNTGIIDLFD NHVDSIPTIL PHQLATLDYL VRTIIDENRS VLLFHIMGSG KTIIALLFAL
     IASRFKKVHI LVPNINILKI FNYNMGVAMN LFNDEFIAEN IFIHSTTSFY SLNYNDNVIN
     YNGLSRYNNS IFIVDKAHNI FGNNTGELMT VIKNKNKIPF LLLSGSPITN TPNTLGHIID
     LMSEETIDFG EIISRGKKVI QTLLNERGVN VLKDLLKGRI SYYEMPDKDL PTIRYHGRKF
     LDTRVVYCHM SKLQERDYMI TRRQLCYHEM FDKNMYNVSM AVLGQLNLMN NLDTLFQEQD
     KELYPNLKIN NGVLYGEELV TLNISSKFKY FINRIQTLNG KHFIYFSNST YGGLVIKYIM
     LSNGYSEYNG SQGTNPHMIN GKPKTFAIVT SKMKSSLEDL LDVYNSPEND DGSQLMFLFS
     SNIMSESYTL KEVRHIWFMT IPDTFSQYNQ ILGRSIRKFS YVDISEPVNV YLLAAVYSDF
     NDEVTSLNDY TQDELINVLP FDIKKLLYLK FKTKETNRIY SILQEMSETY SLPPHPSIVK
     VLLGELVRQF FYNNSRIKYN DAKLLKMVTS VIKNKEDARN YIDDIVNGHF FVSNKVFDKS
     LLYKYENDII TVPFRLSYEP FVWGVNFRKE YNVVSSP