ID   ETF2_MSEPV              Reviewed;         760 AA.
AC   Q9YW29;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   29-SEP-2021, entry version 68.
DE   RecName: Full=Early transcription factor 82 kDa subunit;
DE   AltName: Full=ETF large subunit;
GN   Name=VETFL; OrderedLocusNames=MSV063;
OS   Melanoplus sanguinipes entomopoxvirus (MsEPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Entomopoxvirinae; Deltaentomopoxvirus.
OX   NCBI_TaxID=83191;
OH   NCBI_TaxID=65742; Melanoplus sanguinipes (Migratory grasshopper).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Tucson;
RX   PubMed=9847359; DOI=10.1128/jvi.73.1.533-552.1999;
RA   Afonso C.L., Tulman E.R., Lu Z., Oma E., Kutish G.F., Rock D.L.;
RT   "The genome of Melanoplus sanguinipes entomopoxvirus.";
RL   J. Virol. 73:533-552(1999).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from early
CC       gene promoters. Is recruited by the RPO-associated protein of 94 kDa
CC       (RAP94) to form the early transcription complex, which also contains
CC       the core RNA polymerase. ETF heterodimer binds to early gene promoters
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a 70 kDa and a 82 kDa subunit. Part of the
CC       early transcription complex composed of ETF, RAP94, and the DNA-
CC       directed RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae VETF large subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AF063866; AAC97622.1; -; Genomic_DNA.
DR   PIR; T28224; T28224.
DR   RefSeq; NP_048134.1; NC_001993.1.
DR   SMR; Q9YW29; -.
DR   PRIDE; Q9YW29; -.
DR   GeneID; 1449885; -.
DR   KEGG; vg:1449885; -.
DR   Proteomes; UP000172353; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007532; Poxvirus_early-TF_lsu.
DR   Pfam; PF04441; Pox_VERT_large; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation; Virion.
FT   CHAIN           1..760
FT                   /note="Early transcription factor 82 kDa subunit"
FT                   /id="PRO_0000099086"
SQ   SEQUENCE   760 AA;  89244 MW;  2286DBF5A59A3D98 CRC64;
     MDYIETFINP QVIFLYNENK KIIKTIFLSK DSMIEDSHVM TVYNYLLSSH SDIFENKPQF
     INDHVILTFT LEQARGYIKN ILGLSDDIIL FSIWNNFDYY LKNGIFDPYN IQNMLLVEPN
     DDKSILYNIY QNIVEGAVFC VTTNKNIGSQ LARSNVYSSV YRDYISEIIN NIYKNRYAMK
     SSIIDAMEYS INIDFQDLLR ISNIPDYNKT LCISAYNNFY IKGNKITILD NFDSMYESKY
     LKIYDKNINI NIYDDVLYVK KLSDFTDILN KYNIKSINIK SLKPKTTIYV YFNTFLNPNL
     TIEFDFDFFI NDTKKTKNIF IDLTGKINIM TSQSHISYRS YNISTDLAKY FTLFILGYSH
     IFNKVQSKAK IKKIDELYPS RYCQNYKDIK RQPILIDHID ESRLIKISDN FYTGKEDSTK
     TYQRKGTKKV YDPLKHGDVY IDSNGLKYQC SSIYYSNMGF LGNIYSATGI CYPCCYSKPK
     IRDTIFNTCV HGTKFIHEEK INPIIVNYGR LILNPSNLSN LPYKLNLLLN SKNQIRIVKH
     TNRIILAKNY TVIMAYQPST IIKDFNEMYN FIIENNAILF NDNMMYTHKD IIENNNITPS
     IFILIQNRIH QFKNINKEQD NDNITVSIVD SNKINLIKNK FSVLNNITKE ISENGITVKN
     GKCYIDGELV KNKNITYFTK FSNITIRPKS ISEYILKYFK KYIDDNIYTD NYFLFNKIFV
     TNILNIMHEN DAILNDFSVI QKKLDDMSSD YIVTQSKTYN