ID   ETF2_VACCA              Reviewed;         710 AA.
AC   O57220; Q6J3B2;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   07-APR-2021, entry version 62.
DE   RecName: Full=Early transcription factor 82 kDa subunit;
DE   AltName: Full=ETF large subunit;
DE   AltName: Full=VETF A7 subunit;
DE   AltName: Full=Vaccinia virus early transcription factor large subunit;
DE            Short=VETF large subunit;
GN   Name=VETFL; OrderedLocusNames=MVA118L, ACAM3000_MVA_118;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from early
CC       gene promoters. Is recruited by the RPO-associated protein of 94 kDa
CC       (RAP94) to form the early transcription complex, which also contains
CC       the core RNA polymerase. ETF heterodimer binds to early gene promoters
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a 70 kDa and a 82 kDa subunit. Part of the
CC       early transcription complex composed of ETF, RAP94, and the DNA-
CC       directed RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae VETF large subunit family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U94848; AAB96460.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10516.1; -; Genomic_DNA.
DR   PIR; T37394; T37394.
DR   SMR; O57220; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007532; Poxvirus_early-TF_lsu.
DR   Pfam; PF04441; Pox_VERT_large; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Late protein; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..710
FT                   /note="Early transcription factor 82 kDa subunit"
FT                   /id="PRO_0000099079"
SQ   SEQUENCE   710 AA;  82331 MW;  E3DCF437C4C66AFE CRC64;
     MRYIVSPQLV LQVGKGQEVE RALYLTPYDY IDEKSPIYYF LRSHLNIQQP EIVKRHILLT
     LRMTQLKGYL GNLLDIKDDI IIYSHKNNLE YSYVDNTIFN PFVYTQKKTL LKNDSFLYNV
     YPGACDFLVI WVARACDTSI PEFGSYEDVD NNIIKFETML MEVFPQLDLD ITVESKFNNI
     FRTNLKLTGL KKIIQRVQDL DINYKSLLSR YDEHFINMTG NHFILNDEQL NLSIWDLDGT
     LALSSDGDTV MINNVKLFTD LVSDIDTQME RIKGDITYKV HLATPINSRI KLDIETSFIF
     IETATNNILL SSDKKISIIL AKNHISIKVK NHIPNIEKYF TFLVIAINAM FNSVQKSADF
     TKVETVYWSR ICQNTKNKNR KPIIINYLDP GMKKISNNFY RSDEKEVFIN DNGIMFTCMD
     PLGKYNKVGF LNIFHDMWKY CIPCCFLHDQ SHRSTFSSCV HQIDVEKKIV SPYILNFGKV
     VTESKMSFLP IIFDAFLNDG MTANMEQDNK RLKETSGYHI VRCCAGDDIV RLRTTSDIIQ
     FVNEDKNILI VNDMVYFPMN ASDIGKKIHI LIQEIVHEVM IVKKKESSDK IDFFPPNYKL
     LKDLFPKQTI QTPIQSDAGM VLTTDGFYID GKLFNEDLSS KYVTFTKNVI ASDAVAKYFS
     PLFKYVISEA KDRFIKTWMI NIMIHMNVDP NNIIPTLEKY YPNSGRAQIN