ID   ETF2_VACCW              Reviewed;         710 AA.
AC   P20636; Q76ZQ7; Q85321;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 3.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Early transcription factor 82 kDa subunit;
DE   AltName: Full=ETF large subunit;
DE   AltName: Full=VETF A7 subunit;
DE   AltName: Full=Vaccinia virus early transcription factor large subunit;
DE            Short=VETF large subunit;
GN   Name=VETFL; OrderedLocusNames=VACWR126; ORFNames=A7L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-541.
RX   PubMed=3196167; DOI=10.1007/bf01315559;
RA   van Meir E., Wittek R.;
RT   "Fine structure of the vaccinia virus gene encoding the precursor of the
RT   major core protein 4 a.";
RL   Arch. Virol. 102:19-27(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 526-710.
RX   PubMed=1731116; DOI=10.1128/jvi.66.2.971-982.1992;
RA   Ahn B.-Y., Rosel J., Cole N.B., Moss B.;
RT   "Identification and expression of rpo19, a vaccinia virus gene encoding a
RT   19-kilodalton DNA-dependent RNA polymerase subunit.";
RL   J. Virol. 66:971-982(1992).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-15, AND CHARACTERIZATION.
RX   PubMed=2190222; DOI=10.1073/pnas.87.11.4401;
RA   Gershon P.D., Moss B.;
RT   "Early transcription factor subunits are encoded by vaccinia virus late
RT   genes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:4401-4405(1990).
RN   [5]
RP   DNA-BINDING.
RX   PubMed=1869572; DOI=10.1016/s0021-9258(18)98651-5;
RA   Broyles S.S.;
RT   "A role for ATP hydrolysis in vaccinia virus early gene transcription.
RT   Dissociation of the early transcription factor-promoter complex.";
RL   J. Biol. Chem. 266:15545-15548(1991).
RN   [6]
RP   IDENTIFICATION IN A COMPLEX WITH RAP94 AND THE RNA POLYMERASE.
RX   PubMed=19759131; DOI=10.1128/jvi.01653-09;
RA   Yang Z., Moss B.;
RT   "Interaction of the vaccinia virus RNA polymerase-associated 94-kilodalton
RT   protein with the early transcription factor.";
RL   J. Virol. 83:12018-12026(2009).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from early
CC       gene promoters. Is recruited by the RPO-associated protein of 94 kDa
CC       (RAP94) to form the early transcription complex, which also contains
CC       the core RNA polymerase. ETF heterodimer binds to early gene promoters.
CC   -!- SUBUNIT: Heterodimer of a 70 kDa and a 82 kDa subunit. Part of the
CC       early transcription complex composed of ETF, RAP94, and the DNA-
CC       directed RNA polymerase. {ECO:0000269|PubMed:19759131}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae VETF large subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AY243312; AAO89405.1; -; Genomic_DNA.
DR   EMBL; M27634; AAA69587.1; -; Genomic_DNA.
DR   EMBL; M76473; AAA48338.1; -; Genomic_DNA.
DR   PIR; C43497; C43497.
DR   RefSeq; YP_233008.1; NC_006998.1.
DR   SMR; P20636; -.
DR   DNASU; 3707524; -.
DR   GeneID; 3707524; -.
DR   KEGG; vg:3707524; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007532; Poxvirus_early-TF_lsu.
DR   Pfam; PF04441; Pox_VERT_large; 1.
PE   1: Evidence at protein level;
KW   Activator; Direct protein sequencing; DNA-binding; Late protein;
KW   Reference proteome; Transcription; Transcription regulation; Virion.
FT   CHAIN           1..710
FT                   /note="Early transcription factor 82 kDa subunit"
FT                   /id="PRO_0000099082"
FT   CONFLICT        534..541
FT                   /note="TTSDIIQF -> LHLILFSC (in Ref. 2; AAA69587)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   710 AA;  82273 MW;  44583777C91A0578 CRC64;
     MRYIVSPQLV LQVGKGQEVE RALYLTPYDY IDEKSPIYYF LRSHLNIQQP EIVKRHILLT
     LRMTQLKGYL GNLLDIKDDI IIYSHKNNLE YSYVDNTIFN PFVYTQKKTL LKNDSFLYNV
     YPGACDFLVI WVARACDTSI PEFGSYEDVD NNIIKFETML MEVFPQLDLD ITVESKFNNI
     FRTNLKLTGL KKIIQRVQDL DINYKSLLSR YDEHFINMTG NHFILNDEQL NLSIWDLDGT
     LALSSDGDTV MINNVKLFTD LVSDIDTQME RIKGDITYKV HLATPINSRI KLDIETSFIF
     IETATNNILL SSDKKISIIL AKNHISIKVK NHIPNIEKYF TFLVIAINAM FNSVQKSADF
     TKVETVYWSR ICQNTKNKNR KPIIINYLDP GMKKISNNFY KSDEKEVFIN DNGIMFTCMD
     PLGKYNKVGF LNIFHDMRKY CIPCCFLHDQ SHRSTFSSCV HQIDVEKKIV SPYILNFGKV
     VTESKMSFLP IIFDAFLNDG MTANMEQDNK RLKETSGYHI VRCCAGDDIV RLRTTSDIIQ
     FVNEDKNILI VNDMVYFPMN ASDIGKKIHI LIQEIVHEVM IVKKKESSDK IDFFPPNYKL
     LKDLFPKQTI QTPIQSDAGM VLTTDGFYID GKLFNEDLSS KYVTFTKNVI ASDAVAKYFS
     PLFKYVISEA KDRFIKTWMI NIMIHMNVDP NNIIPTLEKY YPNSGRAQIN