ID   ETF2_VAR67              Reviewed;         710 AA.
AC   P0DOU7; P33806; Q89178;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 8.
DE   RecName: Full=Early transcription factor 82 kDa subunit;
DE   AltName: Full=ETF large subunit;
DE   AltName: Full=VETF A7 subunit;
GN   Name=VETFL; ORFNames=A7L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from early
CC       gene promoters. Is recruited by the RPO-associated protein of 94 kDa
CC       (RAP94) to form the early transcription complex, which also contains
CC       the core RNA polymerase. ETF heterodimer binds to early gene promoters
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a 70 kDa and a 82 kDa subunit. Part of the
CC       early transcription complex composed of ETF, RAP94, and the DNA-
CC       directed RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae VETF large subunit family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA49052.1; -; Genomic_DNA.
DR   EMBL; X67116; CAA47515.1; -; Genomic_DNA.
DR   PIR; H36848; H36848.
DR   RefSeq; NP_042155.1; NC_001611.1.
DR   SMR; P0DOU7; -.
DR   GeneID; 1486537; -.
DR   KEGG; vg:1486537; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007532; Poxvirus_early-TF_lsu.
DR   Pfam; PF04441; Pox_VERT_large; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation; Virion.
FT   CHAIN           1..710
FT                   /note="Early transcription factor 82 kDa subunit"
FT                   /id="PRO_0000099083"
SQ   SEQUENCE   710 AA;  82477 MW;  BAF31D58BF8948F9 CRC64;
     MRYIVSPQLV LQVGKGQEVE RALYLTPYDY IDEKSPIYYF LRSHLNIQQP EIVKRHILLT
     LRMTQLKGYL GNLLDIKDDI IIYSHKNNLE YSYVDNTIFN PFVYTQKKTL LKNDSFLYNV
     YPGACDFLVI WVARACDTSI PEFGSYEDVD NNIIKFETML MDVFPQLDLD ITVESKFNNI
     FRTNLKLTGL KKIIQRVQDL DINYKSLLSR YDEHFINMTG NHFILNDEQL NLSIWDLDGT
     LALSSDGDTV MINNVKLFTD LVSDIDTQME RIKGDITYKV HLATPINSRI KLDIETSFIF
     IETATNNILL SSDKKISIIL AKNHISIKVK NHIPNIEKYF TFLVIAINAM FNSVQKSADF
     TKVETVYWSR ICQNTKNKNR KPVIINYLDP GMKKISNNFY RSDEKEVFIN DNDIMFTCMD
     PLGKYNKVGF LNIFHDMRKY CIPCCFLHDQ SHRSTFSSCV HQIDVEKKIV SPYILNFGKV
     VTESKMSFLP IIFDAFLNDG MTANMEQDNK RLKETSGYHI VRCCTGNDIV RLRTTSNIIQ
     FVNEDKNILI VNDMVYFPMN ASDIGKKIHI LIQEIVHEVM IVKKKESSDK IDFFPPNYKL
     LKDLFPKQTI QTPIQSDAGM VLTTDGFYID GKLFNEDLSS KYVTFTKNVI TSDAVAKYFS
     PLFKYVISEA KDRFIKTWMI NIMIHMNVDP NNIIPTLEKY YPNFGRVQIN