ID   ETFA_BRADU              Reviewed;         314 AA.
AC   P53573;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Electron transfer flavoprotein subunit alpha;
DE            Short=Alpha-ETF;
DE   AltName: Full=Electron transfer flavoprotein large subunit;
DE            Short=ETFLS;
GN   Name=etfA; Synonyms=etfL; OrderedLocusNames=blr1378;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 3I1b110;
RX   PubMed=8599534; DOI=10.1007/bf01692858;
RA   Weidenhaupt M., Rossi P., Beck C., Fischer H.-M., Hennecke H.;
RT   "Bradyrhizobium japonicum possesses two discrete sets of electron transfer
RT   flavoprotein genes: fixA, fixB and etfS, etfL.";
RL   Arch. Microbiol. 165:169-178(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: The electron transfer flavoprotein serves as a specific
CC       electron acceptor for other dehydrogenases. It transfers the electrons
CC       to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF
CC       dehydrogenase) (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC       Note=Binds 1 FAD per dimer. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta subunit.
CC   -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC       {ECO:0000305}.
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DR   EMBL; U32230; AAB00907.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC46643.1; -; Genomic_DNA.
DR   RefSeq; NP_768018.1; NC_004463.1.
DR   RefSeq; WP_011084196.1; NZ_CP011360.1.
DR   AlphaFoldDB; P53573; -.
DR   SMR; P53573; -.
DR   STRING; 224911.27349629; -.
DR   EnsemblBacteria; BAC46643; BAC46643; BAC46643.
DR   GeneID; 64021247; -.
DR   KEGG; bja:blr1378; -.
DR   PATRIC; fig|224911.44.peg.800; -.
DR   eggNOG; COG2025; Bacteria.
DR   HOGENOM; CLU_034178_0_0_5; -.
DR   InParanoid; P53573; -.
DR   OMA; WRPYAEQ; -.
DR   PhylomeDB; P53573; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IBA:GO_Central.
DR   CDD; cd01715; ETF_alpha; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR014730; ETF_a/b_N.
DR   InterPro; IPR001308; ETF_a/FixB.
DR   InterPro; IPR033947; ETF_alpha_N.
DR   InterPro; IPR014731; ETF_asu_C.
DR   InterPro; IPR018206; ETF_asu_C_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR43153; PTHR43153; 1.
DR   Pfam; PF01012; ETF; 1.
DR   Pfam; PF00766; ETF_alpha; 1.
DR   PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR   SMART; SM00893; ETF; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   PROSITE; PS00696; ETF_ALPHA; 1.
PE   3: Inferred from homology;
KW   Electron transport; FAD; Flavoprotein; Reference proteome; Transport.
FT   CHAIN           1..314
FT                   /note="Electron transfer flavoprotein subunit alpha"
FT                   /id="PRO_0000167846"
FT   BINDING         253..281
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        70
FT                   /note="A -> G (in Ref. 1; AAB00907)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   314 AA;  32206 MW;  25426B95EF2B95D7 CRC64;
     MTTLLIAEHD NASLKDATNK ALTAAAALGA DVEVLVAGEG AKAAADAAAK LAGVKKVLLA
     DGALYAHDLA EPLAALIVSL APSYDAIVAP ATSRFKNVMP RVAALLDVMQ VSEIIKVVAP
     DTYERPIYAG NAIQTVKSKD AKKVITVRTS TFAAAGEGGS APVESVQAAA DPGLSSFVGE
     EVAKSDRPEL TSAKIIVSGG RAMQSRENFA KYIEPLADKL GAGVGASRAA VDAGYAPNDW
     QVGQTGKVVA PELYVAVGIS GAIQHLAGMK DSKVIVAINK DEDAPIFQVA DYGLVADLYQ
     AVPELTAELG KLGK