ETFB_PARDE
ID ETFB_PARDE Reviewed; 252 AA.
AC P38975;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Electron transfer flavoprotein subunit beta;
DE Short=Beta-ETF;
DE AltName: Full=Electron transfer flavoprotein small subunit;
DE Short=ETFSS;
GN Name=etfB;
OS Paracoccus denitrificans.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Paracoccus.
OX NCBI_TaxID=266;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-7.
RC STRAIN=ATCC 13543 / NRRL B-3784 / NRC 449;
RX PubMed=8376381; DOI=10.1016/s0021-9258(20)80716-9;
RA Bedzyk L.A., Escudero K.W., Gill R.E., Griffin K.J., Frerman F.E.;
RT "Cloning, sequencing, and expression of the genes encoding subunits of
RT Paracoccus denitrificans electron transfer flavoprotein.";
RL J. Biol. Chem. 268:20211-20217(1993).
RN [2]
RP PROTEIN SEQUENCE OF 1-23; 33-51; 74-82; 163-183 AND 189-197.
RC STRAIN=ATCC 13543 / NRRL B-3784 / NRC 449;
RX PubMed=1576992; DOI=10.1111/j.1432-1033.1992.tb16877.x;
RA Watmough N.J., Kiss J., Frerman F.E.;
RT "Structural and redox relationships between Paracoccus denitrificans,
RT porcine and human electron-transferring flavoproteins.";
RL Eur. J. Biochem. 205:1089-1097(1992).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX PubMed=10026281; DOI=10.1021/bi9820917;
RA Roberts D.L., Salazar D., Fulmer J.P., Frerman F.E., Kim J.-J.P.;
RT "Crystal structure of Paracoccus denitrificans electron transfer
RT flavoprotein: structural and electrostatic analysis of a conserved flavin
RT binding domain.";
RL Biochemistry 38:1977-1989(1999).
CC -!- FUNCTION: The electron transfer flavoprotein serves as a specific
CC electron acceptor for other dehydrogenases. It transfers the electrons
CC to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF
CC dehydrogenase).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Note=Binds 1 FAD per dimer.;
CC -!- COFACTOR:
CC Name=AMP; Xref=ChEBI:CHEBI:456215;
CC Note=Binds 1 AMP per subunit.;
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit.
CC -!- SIMILARITY: Belongs to the ETF beta-subunit/FixA family. {ECO:0000305}.
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DR EMBL; L14864; AAA03071.1; -; Unassigned_DNA.
DR PIR; A48008; A48008.
DR PDB; 1EFP; X-ray; 2.60 A; B/D=1-252.
DR PDBsum; 1EFP; -.
DR AlphaFoldDB; P38975; -.
DR SMR; P38975; -.
DR DIP; DIP-6158N; -.
DR IntAct; P38975; 1.
DR PRIDE; P38975; -.
DR EvolutionaryTrace; P38975; -.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR CDD; cd01714; ETF_beta; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR000049; ET-Flavoprotein_bsu_CS.
DR InterPro; IPR014730; ETF_a/b_N.
DR InterPro; IPR012255; ETF_b.
DR InterPro; IPR033948; ETF_beta_N.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR21294; PTHR21294; 1.
DR Pfam; PF01012; ETF; 1.
DR PIRSF; PIRSF000090; Beta-ETF; 1.
DR SMART; SM00893; ETF; 1.
DR PROSITE; PS01065; ETF_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; FAD;
KW Flavoprotein; Transport.
FT CHAIN 1..252
FT /note="Electron transfer flavoprotein subunit beta"
FT /id="PRO_0000167884"
FT CONFLICT 10
FT /note="L -> V (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="G -> R (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 163
FT /note="V -> Y (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="Q -> E (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 37..50
FT /evidence="ECO:0007829|PDB:1EFP"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 56..65
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 69..78
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 81..87
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 98..112
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 115..121
FT /evidence="ECO:0007829|PDB:1EFP"
FT TURN 124..126
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 131..139
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 142..152
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 154..163
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 166..180
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 192..198
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 208..211
FT /evidence="ECO:0007829|PDB:1EFP"
FT STRAND 218..225
FT /evidence="ECO:0007829|PDB:1EFP"
FT HELIX 239..243
FT /evidence="ECO:0007829|PDB:1EFP"
SQ SEQUENCE 252 AA; 26673 MW; 00C43128BEA1EDED CRC64;
MKVLVPVKRL IDYNVKARVK SDGSGVDLAN VKMSMNPFDE IAVEEAIRLK EKGQAEEIIA
VSIGVKQAAE TLRTALAMGA DRAILVVAAD DVQQDIEPLA VAKILAAVAR AEGTELIIAG
KQAIDNDMNA TGQMLAAILG WAQATFASKV EIEGAKAKVT REVDGGLQTI AVSLPAVVTA
DLRLNEPRYA SLPNIMKAKK KPLDEKTAAD YGVDVAPRLE VVSVREPEGR KAGIKVGSVD
ELVGKLKEAG VI
