ETFD_ACIAD
ID ETFD_ACIAD Reviewed; 570 AA.
AC P94132;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Probable electron transfer flavoprotein-ubiquinone oxidoreductase;
DE Short=ETF-QO;
DE Short=ETF-ubiquinone oxidoreductase;
DE EC=1.5.5.1;
DE AltName: Full=Electron-transferring-flavoprotein dehydrogenase;
DE Short=ETF dehydrogenase;
GN Name=etfD; OrderedLocusNames=ACIAD1680;
OS Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter.
OX NCBI_TaxID=62977;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9294455; DOI=10.1128/jb.179.18.5935-5942.1997;
RA Williams P.A., Shaw L.E.;
RT "mucK, a gene in Acinetobacter calcoaceticus ADP1 (BD413), encodes the
RT ability to grow on exogenous cis,cis-muconate as the sole carbon source.";
RL J. Bacteriol. 179:5935-5942(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33305 / BD413 / ADP1;
RX PubMed=15514110; DOI=10.1093/nar/gkh910;
RA Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L.,
RA Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N.,
RA Weissenbach J., Marliere P., Cohen G.N., Medigue C.;
RT "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1,
RT a versatile and naturally transformation competent bacterium.";
RL Nucleic Acids Res. 32:5766-5779(2004).
CC -!- FUNCTION: Accepts electrons from ETF and reduces ubiquinone.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + reduced [electron-transfer flavoprotein] = a
CC ubiquinol + H(+) + oxidized [electron-transfer flavoprotein];
CC Xref=Rhea:RHEA:24052, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:9566,
CC Rhea:RHEA-COMP:10685, Rhea:RHEA-COMP:10686, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16389, ChEBI:CHEBI:17976, ChEBI:CHEBI:57692,
CC ChEBI:CHEBI:58307; EC=1.5.5.1;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Note=Binds 1 [4Fe-4S] cluster.;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
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DR EMBL; U87258; AAC27118.1; -; Genomic_DNA.
DR EMBL; CR543861; CAG68525.1; -; Genomic_DNA.
DR RefSeq; WP_004926550.1; NC_005966.1.
DR AlphaFoldDB; P94132; -.
DR SMR; P94132; -.
DR STRING; 62977.ACIAD1680; -.
DR EnsemblBacteria; CAG68525; CAG68525; ACIAD1680.
DR GeneID; 45234070; -.
DR KEGG; aci:ACIAD1680; -.
DR eggNOG; COG0644; Bacteria.
DR eggNOG; COG2440; Bacteria.
DR HOGENOM; CLU_009667_4_1_6; -.
DR OMA; APSWHIV; -.
DR OrthoDB; 1770293at2; -.
DR BioCyc; ASP62977:ACIAD_RS07745-MON; -.
DR Proteomes; UP000000430; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR040156; ETF-QO.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR10617; PTHR10617; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 1.
PE 4: Predicted;
KW 4Fe-4S; Electron transport; FAD; Flavoprotein; Iron; Iron-sulfur;
KW Metal-binding; Oxidoreductase; Reference proteome; Transport; Ubiquinone.
FT CHAIN 1..570
FT /note="Probable electron transfer flavoprotein-ubiquinone
FT oxidoreductase"
FT /id="PRO_0000200681"
FT DOMAIN 530..559
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 13..27
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 515
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 539
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 542
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 545
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
SQ SEQUENCE 570 AA; 62996 MW; 0107DD88E78A7DE5 CRC64;
MITIEREAME FDVVIVGAGP AGLSAAIKIR QLAIENNLPD LSVCVVEKGS EVGAHILSGA
VLEPRAINEL FPNWKEEGAP LNVPVTEDKT YFLMSDEKSQ EAPHWMVPKT MHNDGNYVIS
LGNVVRWLGQ KAEELEVSIF PGFAAAEILY HADGTVKGIQ TGDMGIGKDG EPTHNFAPGY
ELHAKYTLFA EGCRGHLGKR LINKFNLDQD ADPQHYGIGI KELWEIDPAK HKPGLVMHGS
GWPLSETGSS GGWWLYHAEN NQVTLGMIVD LSYENPHMFP FMEMQRWKTH PLIKQYLEGG
KRISYGARAV VKGGLNSLPK LTFPGGCLIG DDAGFLNFAK IKGSHTAMKS GMLCGEAVFE
AIARGVDKGG DLAIARVVEG EDLFDKELTT YTQKFDKSWL KEELHRSRNF GPAMHKFGLW
IGGAFNFVDQ NIFKVPFTLH DLQPDYSALK TQDQATFKPN YPKPDGKLTF DRLSSVFVSN
TVHEENQPSH LKLTDASIPV AVNLPRWDEP AQRYCPAGVY EIVDEGEGNK RFQINAANCV
HCKTCDIKDP SQNITWVTPE GGGGPNYPNM
