ETFD_DICDI
ID ETFD_DICDI Reviewed; 606 AA.
AC Q54XM6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial;
DE Short=ETF-QO;
DE Short=ETF-ubiquinone oxidoreductase;
DE EC=1.5.5.1;
DE AltName: Full=Electron-transferring-flavoprotein dehydrogenase;
DE Short=ETF dehydrogenase;
DE Flags: Precursor;
GN Name=etfdh; ORFNames=DDB_G0278849;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Accepts electrons from ETF and reduces ubiquinone.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + reduced [electron-transfer flavoprotein] = a
CC ubiquinol + H(+) + oxidized [electron-transfer flavoprotein];
CC Xref=Rhea:RHEA:24052, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:9566,
CC Rhea:RHEA-COMP:10685, Rhea:RHEA-COMP:10686, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16389, ChEBI:CHEBI:17976, ChEBI:CHEBI:57692,
CC ChEBI:CHEBI:58307; EC=1.5.5.1;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ETF-QO/FixC family. {ECO:0000305}.
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DR EMBL; AAFI02000024; EAL68026.1; -; Genomic_DNA.
DR RefSeq; XP_642004.1; XM_636912.1.
DR AlphaFoldDB; Q54XM6; -.
DR SMR; Q54XM6; -.
DR STRING; 44689.DDB0267019; -.
DR PaxDb; Q54XM6; -.
DR EnsemblProtists; EAL68026; EAL68026; DDB_G0278849.
DR GeneID; 8621736; -.
DR KEGG; ddi:DDB_G0278849; -.
DR dictyBase; DDB_G0278849; etfdh.
DR eggNOG; KOG2415; Eukaryota.
DR HOGENOM; CLU_009667_4_1_1; -.
DR InParanoid; Q54XM6; -.
DR OMA; GGGWMYH; -.
DR PhylomeDB; Q54XM6; -.
DR Reactome; R-DDI-611105; Respiratory electron transport.
DR PRO; PR:Q54XM6; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022900; P:electron transport chain; IBA:GO_Central.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR040156; ETF-QO.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR10617; PTHR10617; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Electron transport; FAD; Flavoprotein; Iron; Iron-sulfur; Membrane;
KW Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..606
FT /note="Electron transfer flavoprotein-ubiquinone
FT oxidoreductase, mitochondrial"
FT /id="PRO_0000328139"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 59..73
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 551
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 575
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 578
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
FT BINDING 581
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255"
SQ SEQUENCE 606 AA; 67634 MW; C48A9E8CFC1AB152 CRC64;
MLKSFSLIGK NITKNVSLSS SNKFLFGKNH QNMKSIYSSI RFFSSEQELP PRDSDQFDVV
IVGAGPSGLS TAIRLKQLSE KAGKDLRVCV VEKGSEVGSH ILSGAVMDPK ALNELIPDWK
EKGAPLITEV KQDKFYFLTE NRSLRLPTPR LMHNEGNYII SLGNVVRWLG EQAESMGVEV
YPSFAASEVL YHDNGAVRGI ATNDMGIAKD GSLTSNFTRG MELNARLTIF AEGCRGSLTK
GLFEKFNLRD ECEPQTFGLG IKETWEIKPE KHQQGLVIHT LGYPLSDELL GGSFIYHAEN
NTVNLGLVVG LDYSNPYLNP YQEFQKLKLH PMVKDMLEGG TCIQYGARTI NEGGFQSIPK
LVFPGGALVG CTAGFVHVPK VKGSHYAMKT GILAAEAAFP QLISQQEKEQ EQEQDKPSVE
PLLINEYPEE LKKSWVWKEL REVRNYRPSL HWGTIPGLIY GALEMYIFRG HTPWTLSNGK
PDNERLKPAA ECKKIEYKKP DGQITFDLMT SVMRSGTNHE ENQPIHLKVR DMEVAKKVNR
DIYDGPEGRF CPAGVYEWVE GEKGEKELVR NSVFCLHCKT CDIKDPTQNI DFTVPEGGGG
PKYGAM
