ETFS_ASFB7
ID ETFS_ASFB7 Reviewed; 1133 AA.
AC Q89525;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Early transcription factor large subunit homolog {ECO:0000303|PubMed:23041356, ECO:0000303|PubMed:32725217};
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent helicase VETFS homolog {ECO:0000303|PubMed:32725217};
GN OrderedLocusNames=Ba71V-106; ORFNames=D1133L;
OS African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=10498;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8262374; DOI=10.1016/0378-1119(93)90090-p;
RA Yanez R.J., Rodriguez J.M., Boursnell M.E., Rodriguez J.F., Vinuela E.;
RT "Two putative African swine fever virus helicases similar to yeast 'DEAH'
RT pre-mRNA processing proteins and vaccinia virus ATPases D11L and D6R.";
RL Gene 134:161-174(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA Rodriguez J.F., Vinuela E.;
RT "Analysis of the complete nucleotide sequence of African swine fever
RT virus.";
RL Virology 208:249-278(1995).
RN [3]
RP REVIEW.
RX PubMed=23041356; DOI=10.1016/j.virusres.2012.09.014;
RA Rodriguez J.M., Salas M.L.;
RT "African swine fever virus transcription.";
RL Virus Res. 173:15-28(2013).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA Alejo A., Matamoros T., Guerra M., Andres G.;
RT "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL J. Virol. 92:0-0(2018).
RN [5]
RP REVIEW.
RX PubMed=32725217; DOI=10.1042/bst20191108;
RA Cackett G., Sykora M., Werner F.;
RT "Transcriptome view of a killer: African swine fever virus.";
RL Biochem. Soc. Trans. 48:1569-1581(2020).
RN [6]
RP INDUCTION.
RX PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA Dixon L., Werner F.;
RT "The African Swine Fever Virus Transcriptome.";
RL J. Virol. 94:0-0(2020).
CC -!- FUNCTION: Putative initation factor. {ECO:0000250|UniProtKB:P04308}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000250|UniProtKB:P04308};
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:30185597}. Note=Found
CC in association with viral nucleoid. {ECO:0000269|PubMed:30185597}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:32075923}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L10061; AAA42697.1; -; Genomic_DNA.
DR EMBL; U18466; AAA65335.1; -; Genomic_DNA.
DR PIR; JT0665; JT0665.
DR RefSeq; NP_042799.1; NC_001659.2.
DR GeneID; 22220335; -.
DR KEGG; vg:22220335; -.
DR Proteomes; UP000000624; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA-binding; Helicase; Hydrolase; Late protein;
KW Nucleotide-binding; Reference proteome; Transcription;
KW Transcription regulation; Virion.
FT CHAIN 1..1133
FT /note="Early transcription factor large subunit homolog"
FT /id="PRO_0000373107"
FT DOMAIN 52..352
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 524..724
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 281..284
FT /note="DEAH box"
FT BINDING 99..106
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1133 AA; 129268 MW; 80C1C790A70BFF2A CRC64;
MAYPELDAAD FLQQLARRKE FKSLISPPVD QKELIRDLRA HFVQIGGPGC EKGGRAFFPC
DPYASPFPSI KGLQLHNAQL FVQNFQNPNT PYSRLLLNWQ TGTGKSIAAI AIARQFMNHY
MNFIENAPWI FVVGFTRAII QTEMLRRPEL GFVSYKEVAE LHRLLHIAKQ SGSTTSVESR
HLNGFVSTLK RRLTDRNRGG FFQFYGYKEF ASKLFNITSK GEEKNFDVLS LFHRSDEAED
TLNENDISQF VQKISEAETN GLIRVNQKIM EQLRGGLLIA DEIHNVYNIQ ERNNYGIALQ
YVLDAFPPHQ APRAVFMSAT PVTGSVMEYV DLLNLLVPRH ELPNGQPLQR QQLFDSSGHS
VKWKKDALAL VERLSTGRVS FLLDTNTNFY PERIFAGKML SYKDETLPYL HFIECPMSEY
QLETLKQLGP DPKISSNAYS IYDMVFPNPK FSKQTEPKAY GLFNSTETPT ALSMASTDWL
LENGVQIIEP SRRAPFNVSG SFLSLQPPTH ISGLAFYSGK YTQMMKDILS IIRQGRGKIL
IYHNRVRMSG VLILQEILQS NGILNEVSSP VGTTRCSICA AIRDEHTHSD HQFIPVRFTI
LHSEIEPAVR ERSLALFNAS SNLEGHQLRI LIGSKVIVEG LNFQAVRYEM IMSLPLDIPR
LIQVFGRVVR KNSHMELPPS ERNVTIYLYV STTPDGGPEL AKYAQKLKEY ILIQEGDKAL
RKHAIDGFTN QIKIDKPMLE SLPLSPSITP ANVGATVLNT FEAYGYGEQE VKTISNIIIS
LFMARPVWTY SELWKAVSTP KLIQGITIDN KLFSEDNFAL ALISLCYSKN QCKELWIQNR
LCTIMHVPAK PEHLYVAAVL NHKKEPVLDI ETYIRDFQLP AMHSIRITKY LEHSQTKEPF
QVLYEKFQKD FQDEPMEQVL IHYPASFHYT MLEALIIDNL AGMGALVEVY KKFFIAFSKK
DIQPFPDIFK IISHVPGDDN TLVGYATEDS VRLITSREDK TWHEIPLYML NINVKRKEND
IVIGYMESKG KALKFKIRPP IQVLKKNEIT DIRMLNRGAV CETRGREEQQ KIADQLGISL
NLTKISAIKL CLLIRNNLLQ KEMEARNQPN GMQDGIRWFY LFNDKMPSLV HTS
