ETHD_METRA
ID ETHD_METRA Reviewed; 142 AA.
AC E9F647;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 2.
DT 25-MAY-2022, entry version 34.
DE RecName: Full=Conidial pigment biosynthesis dehydratase EthD {ECO:0000303|PubMed:28447400};
DE EC=1.-.-.- {ECO:0000305|PubMed:25445307};
GN Name=EthD {ECO:0000303|PubMed:28447400}; ORFNames=MAA_07746;
OS Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS anisopliae (strain ARSEF 23)).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=655844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA Wang C.;
RT "Genome sequencing and comparative transcriptomics of the model
RT entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL PLoS Genet. 7:E1001264-E1001264(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ARSEF 23 / ATCC MYA-3075;
RX PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA St Leger R.J., Wang C.;
RT "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT adaptation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN [3]
RP FUNCTION.
RX PubMed=20382249; DOI=10.1016/j.fgb.2010.03.011;
RA Fang W., Fernandes E.K., Roberts D.W., Bidochka M.J., St Leger R.J.;
RT "A laccase exclusively expressed by Metarhizium anisopliae during isotropic
RT growth is involved in pigmentation, tolerance to abiotic stresses and
RT virulence.";
RL Fungal Genet. Biol. 47:602-607(2010).
RN [4]
RP FUNCTION.
RX PubMed=25445307; DOI=10.1016/j.fgb.2014.10.018;
RA Chen Y., Feng P., Shang Y., Xu Y.J., Wang C.;
RT "Biosynthesis of non-melanin pigment by a divergent polyketide synthase in
RT Metarhizium robertsii.";
RL Fungal Genet. Biol. 81:142-149(2015).
RN [5]
RP IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=28447400; DOI=10.1111/1462-2920.13777;
RA Zeng G., Chen X., Zhang X., Zhang Q., Xu C., Mi W., Guo N., Zhao H.,
RA You Y., Dryburgh F.J., Bidochka M.J., St Leger R.J., Zhang L., Fang W.;
RT "Genome-wide identification of pathogenicity, conidiation and colony
RT sectorization genes in Metarhizium robertsii.";
RL Environ. Microbiol. 19:3896-3908(2017).
RN [6]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=29958281; DOI=10.1371/journal.pgen.1007472;
RA Zeng G., Zhang P., Zhang Q., Zhao H., Li Z., Zhang X., Wang C., Yin W.B.,
RA Fang W.;
RT "Duplication of a Pks gene cluster and subsequent functional
RT diversification facilitate environmental adaptation in Metarhizium
RT species.";
RL PLoS Genet. 14:E1007472-E1007472(2018).
CC -!- FUNCTION: Dehydratase; part of the Pks1 gene cluster that mediates the
CC biosynthesis of an anthraquinone derivative pigment that contributes to
CC conidial pigmentation that provides protection from UV radiation, heat
CC and cold stress (PubMed:28447400). The polyketide synthase Pks1
CC produces 1-acetyl-2,4,6,8-tetrahydroxy-9,10-anthraquinone though
CC condensation of acetyl-CoA with malonyl-CoA (Probable). The dehydratase
CC EthD and the laccase Mlac1 further convert the anthraquinone derivative
CC into the final conidial pigment (Probable).
CC {ECO:0000269|PubMed:28447400, ECO:0000305|PubMed:29958281}.
CC -!- PATHWAY: Pigment biosynthesis. {ECO:0000269|PubMed:28447400}.
CC -!- INDUCTION: Highly expressed during conidiation (PubMed:28447400). A
CC conserved conidiation regulatory pathway containing BrlA, AbaA and WetA
CC regulates expression. During conidiation BlrA up-regulates AbaA, which
CC in turn controls WetA. Moreover, the Hog1 MAPK regulates fungal
CC conidiation by controlling the conidiation regulatory pathway, and that
CC all three pigmentation genes Pks1, EthD and Mlac1 exercise feedback
CC regulation of conidiation (PubMed:28447400).
CC {ECO:0000269|PubMed:28447400}.
CC -!- DISRUPTION PHENOTYPE: Leads to the production of dark red conidia
CC (PubMed:28447400). Does not affect virulence (PubMed:28447400).
CC {ECO:0000269|PubMed:28447400}.
CC -!- SIMILARITY: Belongs to the tpcK family. {ECO:0000305}.
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DR EMBL; ADNJ02000010; EFY96685.2; -; Genomic_DNA.
DR RefSeq; XP_007823935.2; XM_007825744.2.
DR AlphaFoldDB; E9F647; -.
DR EnsemblFungi; EFY96685; EFY96685; MAA_07746.
DR GeneID; 19262032; -.
DR KEGG; maj:MAA_07746; -.
DR HOGENOM; CLU_115019_0_0_1; -.
DR Proteomes; UP000002498; Unassembled WGS sequence.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR009799; EthD_dom.
DR Pfam; PF07110; EthD; 1.
DR SUPFAM; SSF54909; SSF54909; 1.
PE 2: Evidence at transcript level;
KW Monooxygenase; Oxidoreductase.
FT CHAIN 1..142
FT /note="Conidial pigment biosynthesis dehydratase EthD"
FT /id="PRO_0000445753"
FT DOMAIN 25..121
FT /note="EthD"
FT /evidence="ECO:0000255"
SQ SEQUENCE 142 AA; 16524 MW; 1BB0F5A8BB033FA0 CRC64;
MAISDSVSPE PQQLLCLTIT AFRKPGMSEA AYREYMTKTH APLVSGLMEE YGIVRYNMTH
NNSKSRPLLF QLYDPEFSKL SDYDCIVQFV FRRMEDFLRM KSDPRFLEKV APDHQKFADT
SRSTMTIGYF EEFLENGKVV PK