ID   ETHR_MYCBO              Reviewed;         216 AA.
AC   Q7TVI1; A0A1R3Y5G6; X2BPH1;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=HTH-type transcriptional regulator EthR;
GN   Name=ethR; OrderedLocusNames=BQ2027_MB3885;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [3]
RP   FUNCTION AS A REPRESSOR ETHA, AND DISRUPTION PHENOTYPE.
RX   PubMed=10869356; DOI=10.1074/jbc.m003744200;
RA   Baulard A.R., Betts J.C., Engohang-Ndong J., Quan S., McAdam R.A.,
RA   Brennan P.J., Locht C., Besra G.S.;
RT   "Activation of the pro-drug ethionamide is regulated in mycobacteria.";
RL   J. Biol. Chem. 275:28326-28331(2000).
CC   -!- FUNCTION: Involved in the repression of the monooxygenase EthA which is
CC       responsible of the formation of the active metabolite of ethionamide
CC       (ETH). {ECO:0000269|PubMed:10869356}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene leads to ETH
CC       hypersentivity. {ECO:0000269|PubMed:10869356}.
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DR   EMBL; LT708304; SIU02517.1; -; Genomic_DNA.
DR   RefSeq; NP_857522.1; NC_002945.3.
DR   RefSeq; WP_003399797.1; NC_002945.4.
DR   AlphaFoldDB; Q7TVI1; -.
DR   SMR; Q7TVI1; -.
DR   EnsemblBacteria; SIU02517; SIU02517; BQ2027_MB3885.
DR   GeneID; 45427859; -.
DR   PATRIC; fig|233413.5.peg.4256; -.
DR   OMA; TLAHIWV; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..216
FT                   /note="HTH-type transcriptional regulator EthR"
FT                   /id="PRO_0000398579"
FT   DOMAIN          23..83
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        46..65
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            176
FT                   /note="Inhibitor-binding"
FT                   /evidence="ECO:0000250"
FT   SITE            179
FT                   /note="Inhibitor-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   216 AA;  23757 MW;  129DEAB3320514B6 CRC64;
     MTTSAASQAS LPRGRRTARP SGDDRELAIL ATAENLLEDR PLADISVDDL AKGAGISRPT
     FYFYFPSKEA VLLTLLDRVV NQADMALQTL AENPADTDRE NMWRTGINVF FETFGSHKAV
     TRAGQAARAT SVEVAELWST FMQKWIAYTA AVIDAERDRG AAPRTLPAHE LATALNLMNE
     RTLFASFAGE QPSVPEARVL DTLVHIWVTS IYGENR