ID   ETXB_CLOPF              Reviewed;         328 AA.
AC   Q02307;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Epsilon-toxin type B;
DE   Flags: Precursor;
GN   Name=etxB;
OS   Clostridium perfringens.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1502;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 46-65.
RC   STRAIN=NCTC 8533 / NCIMB 8693 / Type B;
RX   PubMed=1729175; DOI=10.1128/iai.60.1.102-110.1992;
RA   Havard H.L., Hunter S.E., Titball R.W.;
RT   "Cloning and nucleotide sequencing of the Clostridium perfringens epsilon-
RT   toxin gene and its expression in Escherichia coli.";
RL   Infect. Immun. 60:102-110(1992).
CC   -!- FUNCTION: Responsible for a rapidly fatal enterotoxemia.
CC   -!- SIMILARITY: To epsilon-toxin type D. {ECO:0000305}.
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DR   EMBL; M95206; AAA23236.1; -; Genomic_DNA.
DR   PDB; 6RB9; EM; 3.20 A; A/B/C/D/E/F/G=47-306.
DR   PDBsum; 6RB9; -.
DR   AlphaFoldDB; Q02307; -.
DR   SMR; Q02307; -.
DR   TCDB; 1.C.5.1.1; the channel-forming Epsilon-toxin (Epsilon-toxin) family.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004991; Aerolysin-like.
DR   InterPro; IPR009209; Epsilon_toxin.
DR   Pfam; PF03318; ETX_MTX2; 1.
DR   PIRSF; PIRSF026587; Epsilon_toxin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Signal; Toxin; Virulence.
FT   SIGNAL          1..32
FT   PROPEP          33..45
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000269|PubMed:1729175"
FT                   /id="PRO_0000021209"
FT   CHAIN           46..328
FT                   /note="Epsilon-toxin type B"
FT                   /id="PRO_0000021210"
FT   STRAND          48..50
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   HELIX           53..69
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   TURN            70..74
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          77..80
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          95..98
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          102..105
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          111..128
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          130..133
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          137..139
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          144..152
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          165..167
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          177..185
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          200..203
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          206..220
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          223..231
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          233..238
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          250..257
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          268..275
FT                   /evidence="ECO:0007829|PDB:6RB9"
FT   STRAND          278..291
FT                   /evidence="ECO:0007829|PDB:6RB9"
SQ   SEQUENCE   328 AA;  36396 MW;  92B6A108D64BC1F9 CRC64;
     MKKNLVKSLA IASAVISIYS IVNIVSPTNV IAKEISNTVS NEMSKKASYD NVDTLIEKGR
     YNTKYNYLKR MEKYYPNAMA YFDKVTINPQ GNDFYINNPK VELDGEPSMN YLEDVYVGKA
     LLTNDTQQEQ KLKSQSFTCK NTDTVTATTT HTVGTSIQAT AKFTVPFNET GVSLTTSYSF
     ANTNTNTNSK EITHNVPSQD ILVPANTTVE VIAYLKKVNV KGNVKLVGQV SGSEWGEIPS
     YLAFPRDGYK FSLSDTVNKS DLNEDGTINI NGKGNYSAVM GDELIVKVRN LNTNNVQEYV
     IPVDKKEKSN DSNIVKYRSL YIKAPGIK