ID   EUPM_PHOSX              Reviewed;         283 AA.
AC   A0A4P8GFD0;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   31-JUL-2019, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=MFS-type transporter eupM {ECO:0000303|PubMed:30980906};
DE   AltName: Full=Eupenifeldin biosynthesis cluster protein M {ECO:0000303|PubMed:30980906};
GN   Name=eupM {ECO:0000303|PubMed:30980906}; ORFNames=gme12630;
OS   Phoma sp.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Phoma.
OX   NCBI_TaxID=1707701;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   PATHWAY.
RC   STRAIN=XZ068 / CGMCC No. 10481;
RX   PubMed=30980906; DOI=10.1016/j.fgb.2019.04.004;
RA   Zhai Y., Li Y., Zhang J., Zhang Y., Ren F., Zhang X., Liu G., Liu X.,
RA   Che Y.;
RT   "Identification of the gene cluster for bistropolone-humulene meroterpenoid
RT   biosynthesis in Phoma sp.";
RL   Fungal Genet. Biol. 129:7-15(2019).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=8360103; DOI=10.7164/antibiotics.46.1082;
RA   Mayerl F., Gao Q., Huang S., Klohr S.E., Matson J.A., Gustavson D.R.,
RA   Pirnik D.M., Berry R.L., Fairchild C., Rose W.C.;
RT   "Eupenifeldin, a novel cytotoxic bistropolone from Eupenicillium
RT   brefeldianum.";
RL   J. Antibiot. 46:1082-1088(1993).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=18095654; DOI=10.1021/np070513k;
RA   Ayers S., Zink D.L., Powell J.S., Brown C.M., Grund A., Bills G.F.,
RA   Platas G., Thompson D., Singh S.B.;
RT   "Noreupenifeldin, a tropolone from an unidentified ascomycete.";
RL   J. Nat. Prod. 71:457-459(2008).
RN   [4]
RP   BIOTECHNOLOGY.
RX   DOI=10.1016/j.phytol.2008.09.008;
RA   Bunyapaiboonsri T., Veeranondha S., Boonruangprapa T., Somrithipol S.;
RT   "Ramiferin, a bisphenol-sesquiterpene from the fungus Kionochaeta ramifera
RT   BCC 7585.";
RL   Phytochem. Lett. 1:204-206(2008).
CC   -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC       the biosynthesis of eupenifeldin, a bistropolone meroterpenoid that
CC       acts as an antitumor agent. {ECO:0000305|PubMed:30980906}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Does not abolish the production of eupenifeldin.
CC       {ECO:0000269|PubMed:30980906}.
CC   -!- BIOTECHNOLOGY: Eupenifeldin is a bistropolone-humulene meroterpenoid
CC       first discovered as an antitumor and anti-leukemia agent
CC       (PubMed:8360103). This metabolite shows also anthelmintic activity
CC       against the parasitic worm Hemonchus contortus, anti-malarial activity
CC       as well as antifungal activity (PubMed:18095654, Ref.4).
CC       {ECO:0000269|PubMed:18095654, ECO:0000269|PubMed:8360103,
CC       ECO:0000269|Ref.4}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
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DR   EMBL; MK400120; QCO93108.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4P8GFD0; -.
DR   SMR; A0A4P8GFD0; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..283
FT                   /note="MFS-type transporter eupM"
FT                   /id="PRO_0000449157"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        263..283
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   283 AA;  30861 MW;  55C89BA37159173E CRC64;
     MSNQHSSTAA PSSYILDSAN QPTSFDGNEQ GDVNPKPLDH IRPVCNLETQ MSNLPTDSVY
     ASQPDGGLVA WLQVLGAWVL FFNTWGAMNS FGVFQTYYES GVLFDRSSSD IAWIGSIQTF
     CLQAMGLVAG PLYDRGGFKI LIVTGSVGVV SGYMMLSLCE EFWQVLLAQG FLIGIAEGCL
     FTPMISILPT YFSTKIGLAT GIASSGSSMG GVVYPIVMKS LMYNIGFAWT TRVLGFISLG
     MLLIPIIVMR ERVKPALRVH RDLIDLSVFT DWPFIVFVIA TMI