ID   EUPR_PHOSX              Reviewed;         466 AA.
AC   A0A4P8GG91;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2020, sequence version 2.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Transcription factor eupR {ECO:0000303|PubMed:30980906};
DE   AltName: Full=Eupenifeldin biosynthesis cluster protein RT {ECO:0000303|PubMed:30980906};
GN   Name=eupR {ECO:0000303|PubMed:30980906}; ORFNames=gme12635;
OS   Phoma sp.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Phoma.
OX   NCBI_TaxID=1707701;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   PATHWAY.
RC   STRAIN=XZ068 / CGMCC No. 10481;
RX   PubMed=30980906; DOI=10.1016/j.fgb.2019.04.004;
RA   Zhai Y., Li Y., Zhang J., Zhang Y., Ren F., Zhang X., Liu G., Liu X.,
RA   Che Y.;
RT   "Identification of the gene cluster for bistropolone-humulene meroterpenoid
RT   biosynthesis in Phoma sp.";
RL   Fungal Genet. Biol. 129:7-15(2019).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=8360103; DOI=10.7164/antibiotics.46.1082;
RA   Mayerl F., Gao Q., Huang S., Klohr S.E., Matson J.A., Gustavson D.R.,
RA   Pirnik D.M., Berry R.L., Fairchild C., Rose W.C.;
RT   "Eupenifeldin, a novel cytotoxic bistropolone from Eupenicillium
RT   brefeldianum.";
RL   J. Antibiot. 46:1082-1088(1993).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=18095654; DOI=10.1021/np070513k;
RA   Ayers S., Zink D.L., Powell J.S., Brown C.M., Grund A., Bills G.F.,
RA   Platas G., Thompson D., Singh S.B.;
RT   "Noreupenifeldin, a tropolone from an unidentified ascomycete.";
RL   J. Nat. Prod. 71:457-459(2008).
RN   [4]
RP   BIOTECHNOLOGY.
RX   DOI=10.1016/j.phytol.2008.09.008;
RA   Bunyapaiboonsri T., Veeranondha S., Boonruangprapa T., Somrithipol S.;
RT   "Ramiferin, a bisphenol-sesquiterpene from the fungus Kionochaeta ramifera
RT   BCC 7585.";
RL   Phytochem. Lett. 1:204-206(2008).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of eupenifeldin, a bistropolone meroterpenoid that
CC       acts as an antitumor agent. {ECO:0000305|PubMed:30980906}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of eupenifeldin.
CC       {ECO:0000269|PubMed:30980906}.
CC   -!- BIOTECHNOLOGY: Eupenifeldin is a bistropolone-humulene meroterpenoid
CC       first discovered as an antitumor and anti-leukemia agent
CC       (PubMed:8360103). This metabolite shows also anthelmintic activity
CC       against the parasitic worm Hemonchus contortus, anti-malarial activity
CC       as well as antifungal activity (PubMed:18095654, Ref.4).
CC       {ECO:0000269|PubMed:18095654, ECO:0000269|PubMed:8360103,
CC       ECO:0000269|Ref.4}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=QCO93113.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; MK400120; QCO93113.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A0A4P8GG91; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..466
FT                   /note="Transcription factor eupR"
FT                   /id="PRO_0000449158"
FT   DNA_BIND        32..62
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   466 AA;  51867 MW;  32D2719697059B7F CRC64;
     MFSTEPRSDT APGSPSCSET KRRKVRRGTH SCWECKRRKV KCSYSNPSDP RCIGCRRRGT
     KCLSQQDVDE GASVVSAAVD EDRRIGDRMV RVEALIEQLA NQVVNRESEK VVGTNDSESD
     LATSCSPQDT QTLKHLLPQK VNSPSTCIEY APVSAALHAA LPPHEDINLI IKAGLDVSLH
     RLMTTSLATL SRCIDGFRAG LAEIPSVDTH PTLLARYLLI LATCLQAAHP ELHAKEIRCL
     SEPPRLAMRR LVNAATDLVT SKDELLSSVE GLECVMLESL YLANDGNLRR AWLVCRRAMA
     VAQLLGLHRV DSQQLPCLTF LSQSVDPRFL WFRIVCTDRQ LCLMLGLPQG TPDVSMATES
     ALANDSASGR FERKQCVIAS RILERNEASG DFTNDDLATV LKLDEELQQA ANEMPSSWWL
     VPNLASSLHD QNKTIWEMLR LIEQMLYFNL LNLLHLPCML RARQPA