ID   EUTA_ECOLI              Reviewed;         467 AA.
AC   P76551; Q2MAJ0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Ethanolamine ammonia-lyase reactivase EutA {ECO:0000305};
GN   Name=eutA; Synonyms=yffT; OrderedLocusNames=b2451, JW2435;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-6, FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / JM109 / ATCC 53323, and K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=15466038; DOI=10.1128/jb.186.20.6845-6854.2004;
RA   Mori K., Bando R., Hieda N., Toraya T.;
RT   "Identification of a reactivating factor for adenosylcobalamin-dependent
RT   ethanolamine ammonia lyase.";
RL   J. Bacteriol. 186:6845-6854(2004).
CC   -!- FUNCTION: Reactivates suicidally inhibited ethanolamine ammonia-lyase
CC       (EAL), cyanocobalamin-inactivated EAL and O(2)-inactivated EAL;
CC       requires Mg(2+), ATP and adenosylcobalamin. Reactivation probably
CC       occurs by the ATP-dependent exchange of cobalamin.
CC       {ECO:0000269|PubMed:15466038}.
CC   -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC   -!- SUBCELLULAR LOCATION: Bacterial microcompartment {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Ethanolamine ammonia-lyase (eutB-eutC) is no
CC       longer reactivated. {ECO:0000269|PubMed:15466038}.
CC   -!- SIMILARITY: Belongs to the EutA family. {ECO:0000305}.
CC   -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC       prophage, encoding 9 genes situated between eutA and eutB, which are
CC       translated in the other direction. CPZ-55 may prevent expression of the
CC       eut operon in strain MG1655. Strain W3110 does not have this prophage
CC       element and should be able to express the operon.
CC       {ECO:0000305|PubMed:9278503}.
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DR   EMBL; U00096; AAC75504.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76716.1; -; Genomic_DNA.
DR   PIR; B65020; B65020.
DR   RefSeq; NP_416946.1; NC_000913.3.
DR   RefSeq; WP_001097542.1; NZ_LN832404.1.
DR   AlphaFoldDB; P76551; -.
DR   BioGRID; 4260579; 13.
DR   BioGRID; 851276; 2.
DR   DIP; DIP-9527N; -.
DR   IntAct; P76551; 5.
DR   STRING; 511145.b2451; -.
DR   PaxDb; P76551; -.
DR   PRIDE; P76551; -.
DR   DNASU; 946937; -.
DR   EnsemblBacteria; AAC75504; AAC75504; b2451.
DR   EnsemblBacteria; BAE76716; BAE76716; BAE76716.
DR   GeneID; 946937; -.
DR   KEGG; ecj:JW2435; -.
DR   KEGG; eco:b2451; -.
DR   PATRIC; fig|1411691.4.peg.4289; -.
DR   EchoBASE; EB3933; -.
DR   eggNOG; COG4819; Bacteria.
DR   HOGENOM; CLU_046255_0_0_6; -.
DR   InParanoid; P76551; -.
DR   OMA; NCDYIDI; -.
DR   PhylomeDB; P76551; -.
DR   BioCyc; EcoCyc:G7281-MON; -.
DR   UniPathway; UPA00560; -.
DR   PRO; PR:P76551; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR   GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0051349; P:positive regulation of lyase activity; IDA:EcoCyc.
DR   GO; GO:0030091; P:protein repair; IDA:EcoCyc.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR009377; EutA.
DR   Pfam; PF06277; EutA; 1.
DR   PIRSF; PIRSF012293; EutA; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
PE   1: Evidence at protein level;
KW   Bacterial microcompartment; Chaperone; Direct protein sequencing;
KW   Reference proteome.
FT   CHAIN           1..467
FT                   /note="Ethanolamine ammonia-lyase reactivase EutA"
FT                   /id="PRO_0000087079"
SQ   SEQUENCE   467 AA;  49599 MW;  8C490FFB24C54B76 CRC64;
     MNTRQLLSVG IDIGTTTTQV IFSRLELVNR AAVSQVPRYE FIKREISWQS PVFFTPVDKQ
     GGLKEAELKT LILEQYHAAG IEPESVDSGA IIITGESAKT RNARPAVMAL SQSLGDFVVA
     SAGPHLESVI AGHGAGAQTL SEQRLCRVLN IDIGGGTANY ALFDAGKISG TACLNVGGRL
     LETDSHGRVV YAHKPGQMIV DECFGAGTDA RSLTGAQLVQ VTRRMAELIV EVIDGTLSPL
     AQALMQTGLL PAGVTPEIIT LSGGVGECYR HQPADPFCFA DIGPLLATAL HDHPRLREMN
     VQFPAQTVRA TVIGAGAHTL SLSGSTIWLE GVQLPLRNLP VAIPIDETDL VGAWQQALIQ
     LDLDPKTDAY VLALPASLPV RYAAVLTVIN ALVDFVARFP NPHPLLVVAG QDFGKALGML
     LRPQLQQLPL AVIDEVIVRA GDYIDIGTPL FGGSVVPVTV KSLAFPS