ID   EUTA_SALTY              Reviewed;         467 AA.
AC   Q9ZFV2;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Ethanolamine ammonia-lyase reactivase EutA;
GN   Name=eutA {ECO:0000303|PubMed:2656649}; OrderedLocusNames=STM2459;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=LT2;
RX   PubMed=10464203; DOI=10.1128/jb.181.17.5317-5329.1999;
RA   Kofoid E.C., Rappleye C.A., Stojiljkovic I., Roth J.R.;
RT   "The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes
RT   five homologues of carboxysome shell proteins.";
RL   J. Bacteriol. 181:5317-5329(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   FUNCTION, PATHWAY, OPERON, AND INDUCTION BY ETHANOLAMINE AND COBALAMIN.
RC   STRAIN=LT2;
RX   PubMed=3045078; DOI=10.1128/jb.170.9.3855-3863.1988;
RA   Roof D.M., Roth J.R.;
RT   "Ethanolamine utilization in Salmonella typhimurium.";
RL   J. Bacteriol. 170:3855-3863(1988).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=LT2;
RX   PubMed=2656649; DOI=10.1128/jb.171.6.3316-3323.1989;
RA   Roof D.M., Roth J.R.;
RT   "Functions required for vitamin B12-dependent ethanolamine utilization in
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 171:3316-3323(1989).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=LT2;
RX   PubMed=15516577; DOI=10.1128/jb.186.22.7635-7644.2004;
RA   Sheppard D.E., Penrod J.T., Bobik T., Kofoid E., Roth J.R.;
RT   "Evidence that a B12-adenosyl transferase is encoded within the
RT   ethanolamine operon of Salmonella enterica.";
RL   J. Bacteriol. 186:7635-7644(2004).
RN   [6]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=LT2;
RX   PubMed=16585748; DOI=10.1128/jb.188.8.2865-2874.2006;
RA   Penrod J.T., Roth J.R.;
RT   "Conserving a volatile metabolite: a role for carboxysome-like organelles
RT   in Salmonella enterica.";
RL   J. Bacteriol. 188:2865-2874(2006).
RN   [7]
RP   FUNCTION.
RC   STRAIN=SL1344;
RX   PubMed=29531136; DOI=10.1128/iai.00172-18;
RA   Anderson C.J., Satkovich J., Koeseoglu V.K., Agaisse H., Kendall M.M.;
RT   "The Ethanolamine Permease EutH Promotes Vacuole Adaptation of Salmonella
RT   enterica and Listeria monocytogenes during Macrophage Infection.";
RL   Infect. Immun. 86:0-0(2018).
CC   -!- FUNCTION: Reactivates suicidally inhibited ethanolamine ammonia-lyase
CC       (EAL), cyanocobalamin-inactivated EAL and O(2)-inactivated EAL;
CC       requires Mg(2+), ATP and adenosylcobalamin. Reactivation probably
CC       occurs by the ATP-dependent exchange of cobalamin (By similarity).
CC       Protects EAL from inhibition by CN-B12, does not have adenosylation
CC       activity (PubMed:15516577) (Probable). {ECO:0000250|UniProtKB:P76551,
CC       ECO:0000269|PubMed:15516577, ECO:0000305|PubMed:10464203,
CC       ECO:0000305|PubMed:2656649}.
CC   -!- FUNCTION: Expression of the eut operon allows this bacteria to use
CC       ethanolamine as a carbon, nitrogen and energy source. It relies on
CC       cobalamin (vitamin B12) both as a cofactor for the ethanolamine
CC       ammonia-lyase (EAL) activity and to induce the operon (PubMed:3045078).
CC       EA enhances bacterial survival in macrophages in a concentration-
CC       dependent manner, suggesting it is an important nutrient during
CC       infection (PubMed:29531136). {ECO:0000269|PubMed:29531136,
CC       ECO:0000269|PubMed:3045078}.
CC   -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC       {ECO:0000269|PubMed:3045078}.
CC   -!- SUBCELLULAR LOCATION: Bacterial microcompartment {ECO:0000305}.
CC   -!- INDUCTION: Part of the 17-gene eut operon transcribed from a single
CC       promoter, induced by ethanolamine and adenosylcobalamin (AdoCbl,
CC       vitamin B12). {ECO:0000269|PubMed:3045078}.
CC   -!- DISRUPTION PHENOTYPE: Can use ethanolamine (EA) as a nitrogen source
CC       when AdoCbl but not cyano-B12 or hydroxy-B12 is provided; cannot use EA
CC       as a carbon source (PubMed:2656649, PubMed:15516577). Required for
CC       aerobic growth on ethanolamine supplemented with cobalamin (vitamin
CC       B12) (PubMed:10464203). A non-polar deletion mutant does not grow on EA
CC       between pH 5.5 and pH 8.5 (PubMed:16585748).
CC       {ECO:0000269|PubMed:10464203, ECO:0000269|PubMed:15516577,
CC       ECO:0000269|PubMed:16585748, ECO:0000269|PubMed:2656649}.
CC   -!- SIMILARITY: Belongs to the EutA family. {ECO:0000305}.
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DR   EMBL; AF093749; AAC78122.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL21353.1; -; Genomic_DNA.
DR   RefSeq; NP_461394.1; NC_003197.2.
DR   RefSeq; WP_001097535.1; NC_003197.2.
DR   AlphaFoldDB; Q9ZFV2; -.
DR   STRING; 99287.STM2459; -.
DR   PaxDb; Q9ZFV2; -.
DR   EnsemblBacteria; AAL21353; AAL21353; STM2459.
DR   GeneID; 1253981; -.
DR   KEGG; stm:STM2459; -.
DR   PATRIC; fig|99287.12.peg.2597; -.
DR   HOGENOM; CLU_046255_0_0_6; -.
DR   OMA; NCDYIDI; -.
DR   PhylomeDB; Q9ZFV2; -.
DR   BioCyc; SENT99287:STM2459-MON; -.
DR   UniPathway; UPA00560; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR   GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR009377; EutA.
DR   Pfam; PF06277; EutA; 1.
DR   PIRSF; PIRSF012293; EutA; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
PE   2: Evidence at transcript level;
KW   Bacterial microcompartment; Chaperone; Reference proteome; Virulence.
FT   CHAIN           1..467
FT                   /note="Ethanolamine ammonia-lyase reactivase EutA"
FT                   /id="PRO_0000087080"
SQ   SEQUENCE   467 AA;  49527 MW;  69F6A052B26938E8 CRC64;
     MNTRQLLSVG IDIGTTTTQV IFSRLELVNR AAVSQVPRYE FIKRDISWQS PVFFTPVDKQ
     GGLKEVELKA LILAQYQAAG IAPESVDSGA IIITGESAKT RNARPAVMAL SQSLGDFVVA
     SAGPHLESVI AGHGAGAQSL SEQRMCRVLN IDIGGGTSNY ALFDAGKVSG TACLNVGGRL
     LETDAQGRVV YAHQPGQMII DEVFGSGTDA RALAAAQLGQ VARRMADLIV EVITGALSPL
     AQSLMQTGLL PADITPEVIT LSGGVGECYR NQPADPFCFS DIGPLLATAL HEHPRLREMN
     VQFPAQTVRA TVIGAGAHTL SLSGSTIWLE DVQLPLRNLP VAIPQDDADL VNAWRQALLQ
     LDLDPQTDAY VLALPATLPV RYAALLTVIN ALTAFVARYP NPHPLLVVAE QDFGKALGML
     LRPQLPQLPL AVIDEVVVRA GDYIDIGTPL FGGSVVPVTV KSLAFPS