EUTE_ECOLI
ID EUTE_ECOLI Reviewed; 467 AA.
AC P77445;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Acetaldehyde dehydrogenase (acetylating) EutE {ECO:0000250|UniProtKB:P41793};
DE EC=1.2.1.10 {ECO:0000250|UniProtKB:P41793};
DE AltName: Full=Ethanolamine utilization protein EutE;
GN Name=eutE; Synonyms=yffX; OrderedLocusNames=b2455, JW2439;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=26384570; DOI=10.1016/j.ymben.2015.09.006;
RA Song J.Y., Park J.S., Kang C.D., Cho H.Y., Yang D., Lee S., Cho K.M.;
RT "Introduction of a bacterial acetyl-CoA synthesis pathway improves lactic
RT acid production in Saccharomyces cerevisiae.";
RL Metab. Eng. 35:38-45(2016).
CC -!- FUNCTION: Acts as the second step in ethanolamine degradation by
CC converting acetaldehyde into acetyl-CoA. May play a role in bacterial
CC microcompartment (BMC) assembly or maintenance. Directly targeted to
CC the BMC (By similarity). Its heterologous expression in S.cerevisiae
CC increases the level of acetylating acetaldehyde dehydrogenase activity
CC (PubMed:26384570). {ECO:0000250|UniProtKB:P41793,
CC ECO:0000269|PubMed:26384570}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetaldehyde + CoA + NAD(+) = acetyl-CoA + H(+) + NADH;
CC Xref=Rhea:RHEA:23288, ChEBI:CHEBI:15343, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.10;
CC Evidence={ECO:0000250|UniProtKB:P41793};
CC -!- COFACTOR:
CC Note=Has a very strong preference for NAD(+) over NADP(+).
CC {ECO:0000250|UniProtKB:P41793};
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC -!- SUBUNIT: Interacts with EutS, which targets it to the interior of the
CC BMC. {ECO:0000250|UniProtKB:P41793}.
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000250|UniProtKB:P41793}. Note=Probably located inside the BMC.
CC {ECO:0000250|UniProtKB:P41793}.
CC -!- SIMILARITY: Belongs to the EutE/PduP family. {ECO:0000305}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; U00096; AAC75508.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16333.1; -; Genomic_DNA.
DR PIR; F65020; F65020.
DR RefSeq; NP_416950.1; NC_000913.3.
DR RefSeq; WP_001075716.1; NZ_LN832404.1.
DR AlphaFoldDB; P77445; -.
DR SMR; P77445; -.
DR BioGRID; 4260567; 8.
DR IntAct; P77445; 4.
DR STRING; 511145.b2455; -.
DR jPOST; P77445; -.
DR PaxDb; P77445; -.
DR PRIDE; P77445; -.
DR EnsemblBacteria; AAC75508; AAC75508; b2455.
DR EnsemblBacteria; BAA16333; BAA16333; BAA16333.
DR GeneID; 946943; -.
DR KEGG; ecj:JW2439; -.
DR KEGG; eco:b2455; -.
DR PATRIC; fig|1411691.4.peg.4285; -.
DR EchoBASE; EB3937; -.
DR eggNOG; COG1012; Bacteria.
DR HOGENOM; CLU_028794_1_0_6; -.
DR InParanoid; P77445; -.
DR OMA; IAIKSRN; -.
DR PhylomeDB; P77445; -.
DR BioCyc; EcoCyc:G7285-MON; -.
DR BioCyc; MetaCyc:G7285-MON; -.
DR UniPathway; UPA00560; -.
DR PRO; PR:P77445; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IDA:EcoCyc.
DR GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IMP:CACAO.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07121; ALDH_EutE; 1.
DR Gene3D; 3.40.309.10; -; 1.
DR Gene3D; 3.40.605.10; -; 1.
DR InterPro; IPR012408; Acetald_propionald_DH-rel.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR Pfam; PF00171; Aldedh; 1.
DR PIRSF; PIRSF036410; EutE_PduP; 1.
DR SUPFAM; SSF53720; SSF53720; 1.
PE 3: Inferred from homology;
KW Bacterial microcompartment; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..467
FT /note="Acetaldehyde dehydrogenase (acetylating) EutE"
FT /id="PRO_0000087084"
SQ SEQUENCE 467 AA; 49022 MW; 88C5D40452218E8D CRC64;
MNQQDIEQVV KAVLLKMQSS DTPSAAVHEM GVFASLDDAV AAAKVAQQGL KSVAMRQLAI
AAIREAGEKH ARDLAELAVS ETGMGRVEDK FAKNVAQARG TPGVECLSPQ VLTGDNGLTL
IENAPWGVVA SVTPSTNPAA TVINNAISLI AAGNSVIFAP HPAAKKVSQR AITLLNQAIV
AAGGPENLLV TVANPDIETA QRLFKFPGIG LLVVTGGEAV VEAARKHTNK RLIAAGAGNP
PVVVDETADL ARAAQSIVKG ASFDNNIICA DEKVLIVVDS VADELMRLME GQHAVKLTAE
QAQQLQPVLL KNIDERGKGT VSRDWVGRDA GKIAAAIGLK VPQETRLLFV ETTAEHPFAV
TELMMPVLPV VRVANVADAI ALAVKLEGGC HHTAAMHSRN IENMNQMANA IDTSIFVKNG
PCIAGLGLGG EGWTTMTITT PTGEGVTSAR TFVRLRRCVL VDAFRIV
