ID   EUTH_LISM4              Reviewed;         373 AA.
AC   A0A0H3GFN0;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Probable ethanolamine permease EutH {ECO:0000303|PubMed:29531136};
DE   AltName: Full=Ethanolamine utilization protein EutH;
GN   Name=eutH {ECO:0000303|PubMed:29531136}; OrderedLocusNames=LMRG_00632;
OS   Listeria monocytogenes serotype 1/2a (strain 10403S).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=393133;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=10403S;
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M.,
RA   Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D.,
RA   Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L.,
RA   Arachchi H.M., Berlin A., Borenstein D., Brown A., Chapman S.B., Chen Z.,
RA   Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J.,
RA   Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L.,
RA   Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D.,
RA   Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T.,
RA   Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C.,
RA   Haas B., Nusbaum C., Birren B.;
RT   "The genome sequence of Listeria monocytogenes strain 10403S.";
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=10403S;
RX   PubMed=29531136; DOI=10.1128/iai.00172-18;
RA   Anderson C.J., Satkovich J., Koeseoglu V.K., Agaisse H., Kendall M.M.;
RT   "The Ethanolamine Permease EutH Promotes Vacuole Adaptation of Salmonella
RT   enterica and Listeria monocytogenes during Macrophage Infection.";
RL   Infect. Immun. 86:0-0(2018).
CC   -!- FUNCTION: Probably involved in the diffusion of protonated ethanolamine
CC       (EA) into the cell at low pH. At low pH most EA is protonated, and this
CC       permease becomes necessary (By similarity). Contributes to bacterial
CC       survival and replication in acidic macrophage vacuoles, but not to
CC       bacterial uptake by macrophages (PubMed:29531136).
CC       {ECO:0000250|UniProtKB:P41796, ECO:0000269|PubMed:29531136}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Decrease in mouse macrophage survival 2-5 hours
CC       after infection. {ECO:0000269|PubMed:29531136}.
CC   -!- SIMILARITY: Belongs to the EutH family. {ECO:0000305}.
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DR   EMBL; CP002002; AEO06169.1; -; Genomic_DNA.
DR   RefSeq; WP_003721584.1; NC_017544.1.
DR   AlphaFoldDB; A0A0H3GFN0; -.
DR   EnsemblBacteria; AEO06169; AEO06169; LMRG_00632.
DR   KEGG; lmt:LMRG_00632; -.
DR   HOGENOM; CLU_061142_0_0_9; -.
DR   OMA; FGDHLGF; -.
DR   Proteomes; UP000001288; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034228; F:ethanolamine transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR007441; EutH.
DR   PANTHER; PTHR40089; PTHR40089; 1.
DR   Pfam; PF04346; EutH; 1.
DR   PIRSF; PIRSF019466; EutH; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport;
KW   Virulence.
FT   CHAIN           1..373
FT                   /note="Probable ethanolamine permease EutH"
FT                   /id="PRO_0000452916"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   373 AA;  38711 MW;  CDCA06E8C5DCB5C3 CRC64;
     MSINEIIIYL MVIFMILGAI DKIIGNKFGL GAQFEEGIMA MGSLTLAMVG IITLAPVLAK
     ILSPIVVPIY TALGADPAMF ATTLLANDMG GFALAQELAL TPDAGLFAGA ILGSMMGPTI
     VFTIPVALGI IKKEDHKYLA TGVLSGIITI PIGCLIGGLV AGFSPIMIFK NLVPIILVAA
     LIMLGLWFKP EGMIKGFTIF GKGVVIVATI GLVAGAIQQL TGLTIIPGIA PIGEGIEIVG
     GIALVLAGAF CLVFVITKVF NKPLMKMGKL LGMNEVAAAG MVATLANSIP MFQMLKDMDE
     RGKIINVAFA VSAAFVLGDH LGFTAGVAQD MIFPMIVGKL VGGVTAVAVG IYMANRMMKK
     NKAKEQTAVK DNG