EUTK_ECOLI
ID EUTK_ECOLI Reviewed; 166 AA.
AC P76540; P77408;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Bacterial microcompartment shell protein EutK;
DE AltName: Full=Ethanolamine utilization protein EutK;
GN Name=eutK; Synonyms=yffI; OrderedLocusNames=b2438, JW2431;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4] {ECO:0007744|PDB:3I71}
RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 108-166, FUNCTION, SUBUNIT, AND
RP SUBCELLULAR LOCATION.
RX PubMed=20044574; DOI=10.1126/science.1179513;
RA Tanaka S., Sawaya M.R., Yeates T.O.;
RT "Structure and mechanisms of a protein-based organelle in Escherichia
RT coli.";
RL Science 327:81-84(2010).
CC -!- FUNCTION: Probably a minor component of the bacterial microcompartment
CC (BMC) shell dedicated to ethanolamine degradation. It might bind
CC nucleic acids. {ECO:0000305|PubMed:20044574}.
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC -!- SUBUNIT: Monomeric in solution. {ECO:0000269|PubMed:20044574}.
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000305|PubMed:20044574}.
CC -!- SIMILARITY: Belongs to the bacterial microcompartments protein family.
CC {ECO:0000255|PROSITE-ProRule:PRU01278}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; U00096; AAC75491.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA16321.1; -; Genomic_DNA.
DR PIR; E65018; E65018.
DR RefSeq; NP_416933.4; NC_000913.3.
DR RefSeq; WP_000606257.1; NZ_STEB01000039.1.
DR PDB; 3I71; X-ray; 2.10 A; A/B=108-166.
DR PDBsum; 3I71; -.
DR AlphaFoldDB; P76540; -.
DR SMR; P76540; -.
DR BioGRID; 851251; 2.
DR IntAct; P76540; 8.
DR STRING; 511145.b2438; -.
DR PaxDb; P76540; -.
DR PRIDE; P76540; -.
DR EnsemblBacteria; AAC75491; AAC75491; b2438.
DR EnsemblBacteria; BAA16321; BAA16321; BAA16321.
DR GeneID; 66673692; -.
DR GeneID; 946912; -.
DR KEGG; ecj:JW2431; -.
DR KEGG; eco:b2438; -.
DR PATRIC; fig|1411691.4.peg.4293; -.
DR EchoBASE; EB3922; -.
DR eggNOG; COG4577; Bacteria.
DR HOGENOM; CLU_064903_7_0_6; -.
DR OMA; WLIGGFK; -.
DR BioCyc; EcoCyc:G7270-MON; -.
DR UniPathway; UPA00560; -.
DR EvolutionaryTrace; P76540; -.
DR PRO; PR:P76540; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0010165; P:response to X-ray; IMP:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.70.1710; -; 1.
DR InterPro; IPR020808; Bact_microcomp_CS.
DR InterPro; IPR000249; BMC_dom.
DR InterPro; IPR037233; CcmK-like_sf.
DR InterPro; IPR044872; CcmK/CsoS1_BMC.
DR InterPro; IPR032298; EutK_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00936; BMC; 1.
DR Pfam; PF16365; EutK_C; 1.
DR SMART; SM00877; BMC; 1.
DR SUPFAM; SSF143414; SSF143414; 1.
DR PROSITE; PS01139; BMC_1; 1.
DR PROSITE; PS51930; BMC_2; 1.
DR PROSITE; PS51933; EUTK_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial microcompartment; Reference proteome.
FT CHAIN 1..166
FT /note="Bacterial microcompartment shell protein EutK"
FT /id="PRO_0000004784"
FT DOMAIN 4..88
FT /note="BMC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01278"
FT DOMAIN 109..165
FT /note="EutK-Ctail"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01281"
FT HELIX 112..120
FT /evidence="ECO:0007829|PDB:3I71"
FT HELIX 127..134
FT /evidence="ECO:0007829|PDB:3I71"
FT HELIX 138..150
FT /evidence="ECO:0007829|PDB:3I71"
FT STRAND 153..157
FT /evidence="ECO:0007829|PDB:3I71"
FT STRAND 160..163
FT /evidence="ECO:0007829|PDB:3I71"
SQ SEQUENCE 166 AA; 17893 MW; 0B3860B549A28FB5 CRC64;
MINALGLLEV DGMVAAIDAA DAMLKAANVR LLSHEVLDPG RLTLVVEGDL AACRAALDAG
CAAAMRTGRV ISRKEIGRPD DDTQWLVTGF NRQPKQPVRE PDAPVIVAES ADELLALLTS
VRQGMTAGEV AAHFGWPLEK ARNALEQLFS AGTLRKRSSR YRLKPH
