EUTN_ECOLI
ID EUTN_ECOLI Reviewed; 95 AA.
AC P0AEJ8; P77633;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Bacterial microcompartment shell vertex protein EutN {ECO:0000303|PubMed:23456886};
DE AltName: Full=Ethanolamine catabolic microcompartment shell protein EutN {ECO:0000305};
DE AltName: Full=Ethanolamine utilization protein EutN;
GN Name=eutN; Synonyms=cchB, yffY; OrderedLocusNames=b2456, JW2440;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBUNIT.
RX PubMed=23456886; DOI=10.1002/pro.2246;
RA Wheatley N.M., Gidaniyan S.D., Liu Y., Cascio D., Yeates T.O.;
RT "Bacterial microcompartment shells of diverse functional types possess
RT pentameric vertex proteins.";
RL Protein Sci. 22:660-665(2013).
RN [5] {ECO:0007744|PDB:2HD3}
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS), FUNCTION, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=17588214; DOI=10.1007/s10969-007-9018-3;
RA Forouhar F., Kuzin A., Seetharaman J., Lee I., Zhou W., Abashidze M.,
RA Chen Y., Yong W., Janjua H., Fang Y., Wang D., Cunningham K., Xiao R.,
RA Acton T.B., Pichersky E., Klessig D.F., Porter C.W., Montelione G.T.,
RA Tong L.;
RT "Functional insights from structural genomics.";
RL J. Struct. Funct. Genomics 8:37-44(2007).
RN [6] {ECO:0007744|PDB:2Z9H}
RP X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS), SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=18292340; DOI=10.1126/science.1151458;
RA Tanaka S., Kerfeld C.A., Sawaya M.R., Cai F., Heinhorst S., Cannon G.C.,
RA Yeates T.O.;
RT "Atomic-level models of the bacterial carboxysome shell.";
RL Science 319:1083-1086(2008).
CC -!- FUNCTION: Probably forms vertices in the bacterial microcompartment
CC (BMC) dedicated to ethanolamine degradation (Probable). It may be
CC involved in transporting positively charged molecules into and out of
CC the BMC (Probable). {ECO:0000305|PubMed:17588214,
CC ECO:0000305|PubMed:23456886}.
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC -!- SUBUNIT: Has been crystallized as a homohexamer with an acidic central
CC pore with a diameter of 26 Angstroms on one side and 14 Angstroms on
CC the other (PubMed:17588214, PubMed:18292340). In solution has been
CC shown to be a pentamer (PubMed:23456886). {ECO:0000269|PubMed:17588214,
CC ECO:0000269|PubMed:18292340, ECO:0000269|PubMed:23456886}.
CC -!- INTERACTION:
CC P0AEJ8; P18196: minC; NbExp=3; IntAct=EBI-8767793, EBI-554060;
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000305|PubMed:17588214, ECO:0000305|PubMed:18292340}.
CC -!- SIMILARITY: Belongs to the CcmL/EutN family. {ECO:0000305}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; AP009048; BAA16334.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75509.1; -; Genomic_DNA.
DR PIR; G65020; G65020.
DR RefSeq; NP_416951.1; NC_000913.3.
DR RefSeq; WP_000762196.1; NZ_STEB01000051.1.
DR PDB; 2HD3; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J/K/L=1-95.
DR PDB; 2Z9H; X-ray; 2.71 A; A/B/C/D/E/F=1-95.
DR PDBsum; 2HD3; -.
DR PDBsum; 2Z9H; -.
DR AlphaFoldDB; P0AEJ8; -.
DR SMR; P0AEJ8; -.
DR BioGRID; 4260582; 12.
DR BioGRID; 851284; 12.
DR IntAct; P0AEJ8; 13.
DR STRING; 511145.b2456; -.
DR PaxDb; P0AEJ8; -.
DR EnsemblBacteria; AAC75509; AAC75509; b2456.
DR EnsemblBacteria; BAA16334; BAA16334; BAA16334.
DR GeneID; 66673683; -.
DR GeneID; 946945; -.
DR KEGG; ecj:JW2440; -.
DR KEGG; eco:b2456; -.
DR PATRIC; fig|1411691.4.peg.4284; -.
DR EchoBASE; EB3938; -.
DR eggNOG; COG4576; Bacteria.
DR HOGENOM; CLU_148498_0_0_6; -.
DR InParanoid; P0AEJ8; -.
DR OMA; GAGINEW; -.
DR PhylomeDB; P0AEJ8; -.
DR BioCyc; EcoCyc:G7286-MON; -.
DR UniPathway; UPA00560; -.
DR EvolutionaryTrace; P0AEJ8; -.
DR PRO; PR:P0AEJ8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0034214; P:protein hexamerization; IDA:EcoCyc.
DR CDD; cd01614; EutN_CcmL; 1.
DR Gene3D; 2.40.50.220; -; 1.
DR InterPro; IPR004992; EutN_CcmL.
DR InterPro; IPR036677; EutN_CcmL_sf.
DR PANTHER; PTHR36539; PTHR36539; 1.
DR Pfam; PF03319; EutN_CcmL; 1.
DR SUPFAM; SSF159133; SSF159133; 1.
DR PROSITE; PS51932; BMV; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial microcompartment; Reference proteome.
FT CHAIN 1..95
FT /note="Bacterial microcompartment shell vertex protein
FT EutN"
FT /id="PRO_0000087091"
FT DOMAIN 1..83
FT /note="BMV"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01280"
FT STRAND 2..11
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:2Z9H"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 23..30
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 36..47
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 54..59
FT /evidence="ECO:0007829|PDB:2Z9H"
FT HELIX 60..67
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 75..81
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 83..89
FT /evidence="ECO:0007829|PDB:2Z9H"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:2Z9H"
SQ SEQUENCE 95 AA; 9956 MW; C727E1EB4E1E4038 CRC64;
MKLAVVTGQI VCTVRHHGLA HDKLLMVEMI DPQGNPDGQC AVAIDNIGAG TGEWVLLVSG
SSARQAHKSE TSPVDLCVIG IVDEVVSGGQ VIFHK
