EUTQ_ECOLI
ID EUTQ_ECOLI Reviewed; 233 AA.
AC P76555; Q2MAI8;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Acetate kinase EutQ;
DE EC=2.7.2.1 {ECO:0000250|UniProtKB:Q9ZFV5};
DE AltName: Full=Ethanolamine utilization protein EutQ;
GN Name=eutQ; Synonyms=ypfC; OrderedLocusNames=b2460, JW2444;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: A bidirectional acetate kinase that may drive flux through
CC the ethanolamine degradation pathway under anoxic conditions. It may
CC generate ATP that can be used by other enzymes (EutA and EutT) in the
CC eut pathway. Might serve as an assembly hub for bacterial
CC microcompartment (BMC) shell proteins. {ECO:0000250|UniProtKB:Q9ZFV5}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetate + ATP = acetyl phosphate + ADP; Xref=Rhea:RHEA:11352,
CC ChEBI:CHEBI:22191, ChEBI:CHEBI:30089, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:456216; EC=2.7.2.1;
CC Evidence={ECO:0000250|UniProtKB:Q9ZFV5};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11353;
CC Evidence={ECO:0000250|UniProtKB:Q9ZFV5};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11354;
CC Evidence={ECO:0000250|UniProtKB:Q9ZFV5};
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q187N7}.
CC -!- INTERACTION:
CC P76555; P77609: flxA; NbExp=3; IntAct=EBI-553018, EBI-553024;
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000250|UniProtKB:Q9ZFV5}. Note=May be found on the cytoplasmic
CC face of the BMC. {ECO:0000250|UniProtKB:Q9ZFV5}.
CC -!- SIMILARITY: Belongs to the EutQ cupin-like family. {ECO:0000305}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; U00096; AAC75513.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76718.1; -; Genomic_DNA.
DR PIR; C65021; C65021.
DR RefSeq; NP_416955.1; NC_000913.3.
DR RefSeq; WP_000733906.1; NZ_LN832404.1.
DR AlphaFoldDB; P76555; -.
DR SMR; P76555; -.
DR BioGRID; 4260918; 12.
DR BioGRID; 851274; 5.
DR DIP; DIP-9540N; -.
DR IntAct; P76555; 12.
DR STRING; 511145.b2460; -.
DR TCDB; 9.B.75.2.1; the ethanol utilization/transport (eut) protein family.
DR jPOST; P76555; -.
DR PaxDb; P76555; -.
DR PRIDE; P76555; -.
DR DNASU; 946935; -.
DR EnsemblBacteria; AAC75513; AAC75513; b2460.
DR EnsemblBacteria; BAE76718; BAE76718; BAE76718.
DR GeneID; 66673679; -.
DR GeneID; 946935; -.
DR KEGG; ecj:JW2444; -.
DR KEGG; eco:b2460; -.
DR PATRIC; fig|511145.12.peg.2554; -.
DR EchoBASE; EB3942; -.
DR eggNOG; COG4766; Bacteria.
DR HOGENOM; CLU_082122_0_0_6; -.
DR InParanoid; P76555; -.
DR OMA; SKVTWSS; -.
DR PhylomeDB; P76555; -.
DR BioCyc; EcoCyc:G7290-MON; -.
DR UniPathway; UPA00560; -.
DR PRO; PR:P76555; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031469; C:bacterial microcompartment; IEA:UniProtKB-SubCell.
DR GO; GO:0008776; F:acetate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR010424; EutQ.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR36169; PTHR36169; 1.
DR Pfam; PF06249; EutQ; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 1: Evidence at protein level;
KW Bacterial microcompartment; Kinase; Reference proteome; Transferase.
FT CHAIN 1..233
FT /note="Acetate kinase EutQ"
FT /id="PRO_0000087097"
SQ SEQUENCE 233 AA; 25418 MW; 92E91B2EB681B2EE CRC64;
MKKLITANDI REAHARGEQA MSVVLRASII TPEAREVADL LGFTITECDE SIPVTASVPA
SVPADKTESQ RIRETIIAQL PEGQFTESLV AQLMEKVMKE KQSLEQGAMQ PSFKSVTGKG
GIKVIDGSSV KFGRFDGAEP HCVGLTDLVT GDDGSSMAAG FMQWENAFFP WTLNYDEIDM
VLEGELHVRH EGQTMIAKAG DVMFIPKGSS IEFGTTSSVK FLYVAWPANW QSL
