EUTR_ECOLI
ID EUTR_ECOLI Reviewed; 350 AA.
AC P36547; P77451;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=HTH-type DNA-binding transcriptional activator EutR {ECO:0000305};
DE AltName: Full=Ethanolamine operon regulatory protein;
GN Name=eutR; Synonyms=yfeG; OrderedLocusNames=b2437, JW2430;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8300522; DOI=10.1128/jb.176.3.673-680.1994;
RA Troup B., Jahn M., Hungerer C., Jahn D.;
RT "Isolation of the hemF operon containing the gene for the Escherichia coli
RT aerobic coproporphyrinogen III oxidase by in vivo complementation of a
RT yeast HEM13 mutant.";
RL J. Bacteriol. 176:673-680(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Activates the transcription of the eut operon. Also
CC positively regulates its own transcription. Probably binds ethanolamine
CC and vitamin B12 as effectors. Competes with ethanolamine ammonia-lysase
CC (EAL, the first enzyme in the EA degradation pathway) for
CC adenosylcobalamin. {ECO:0000250|UniProtKB:Q9ZFU7}.
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC {ECO:0000305}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; X75413; CAA53168.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75490.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16320.1; -; Genomic_DNA.
DR PIR; D65018; D65018.
DR RefSeq; NP_416932.1; NC_000913.3.
DR RefSeq; WP_000753690.1; NZ_LN832404.1.
DR AlphaFoldDB; P36547; -.
DR SMR; P36547; -.
DR IntAct; P36547; 3.
DR STRING; 511145.b2437; -.
DR PaxDb; P36547; -.
DR PRIDE; P36547; -.
DR DNASU; 946911; -.
DR EnsemblBacteria; AAC75490; AAC75490; b2437.
DR EnsemblBacteria; BAA16320; BAA16320; BAA16320.
DR GeneID; 946911; -.
DR KEGG; ecj:JW2430; -.
DR KEGG; eco:b2437; -.
DR PATRIC; fig|1411691.4.peg.4294; -.
DR EchoBASE; EB2107; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_047930_2_0_6; -.
DR OMA; FLHHPER; -.
DR PhylomeDB; P36547; -.
DR BioCyc; EcoCyc:EG12190-MON; -.
DR UniPathway; UPA00560; -.
DR PRO; PR:P36547; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR Pfam; PF12833; HTH_18; 1.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..350
FT /note="HTH-type DNA-binding transcriptional activator EutR"
FT /id="PRO_0000194509"
FT DOMAIN 243..344
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 260..281
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 311..334
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT CONFLICT 276
FT /note="A -> R (in Ref. 1; CAA53168)"
FT /evidence="ECO:0000305"
FT CONFLICT 285
FT /note="P -> R (in Ref. 1; CAA53168)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 350 AA; 40160 MW; 1281A43DC31DE845 CRC64;
MKKTRTANLH HLYHEPLPEN LKLTPKVEVD NVHQRQTTDV YEHALTITAW QQIYDQLHPG
KFHGEFTEIL LDDIQVFREY TGLALRQSCL VWPNSFWFGI PATRGEQGFI GSQCLGSAEI
ATRPGGTEFE LSTPDDYTIL GVVLSEDVIT RQANFLHNPD RVLHMLRNQS ALEVKEQHKA
ALWGFVQQAL ATFCENPENL HQPAVRKVLG DNLLMAMGAM LEEAQPMVTA ESISHQSYRR
LLSRAREYVL ENMSEPVTVL DLCNQLHVSR RTLQNAFHAI LGIGPNAWLK RIRLNAVRRE
LISPWSQSMT VKDAAMQWGF WHLGQFATDY QQLFSEKPSL TLHQRMREWG
