EVA1A_HUMAN
ID EVA1A_HUMAN Reviewed; 152 AA.
AC Q9H8M9; D6W5J3; Q9HC41;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein eva-1 homolog A;
DE AltName: Full=Protein FAM176A;
DE AltName: Full=Transmembrane protein 166;
GN Name=EVA1A; Synonyms=FAM176A, TMEM166; ORFNames=SP24;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17492404; DOI=10.1007/s10495-007-0073-9;
RA Wang L., Yu C., Lu Y., He P., Guo J., Zhang C., Song Q., Ma D., Shi T.,
RA Chen Y.;
RT "TMEM166, a novel transmembrane protein, regulates cell autophagy and
RT apoptosis.";
RL Apoptosis 12:1489-1502(2007).
RN [8]
RP FUNCTION.
RX PubMed=19029833; DOI=10.4161/auto.5.1.7247;
RA He P., Peng Z., Luo Y., Wang L., Yu P., Deng W., An Y., Shi T., Ma D.;
RT "High-throughput functional screening for autophagy-related genes and
RT identification of TM9SF1 as an autophagosome-inducing gene.";
RL Autophagy 5:52-60(2009).
RN [9]
RP PHOSPHORYLATION AT SER-114.
RX PubMed=26091039; DOI=10.1016/j.cell.2015.05.028;
RA Tagliabracci V.S., Wiley S.E., Guo X., Kinch L.N., Durrant E., Wen J.,
RA Xiao J., Cui J., Nguyen K.B., Engel J.L., Coon J.J., Grishin N.,
RA Pinna L.A., Pagliarini D.J., Dixon J.E.;
RT "A single kinase generates the majority of the secreted phosphoproteome.";
RL Cell 161:1619-1632(2015).
CC -!- FUNCTION: Acts as a regulator of programmed cell death, mediating both
CC autophagy and apoptosis. {ECO:0000269|PubMed:17492404,
CC ECO:0000269|PubMed:19029833}.
CC -!- INTERACTION:
CC Q9H8M9; P02724: GYPA; NbExp=3; IntAct=EBI-715362, EBI-702665;
CC Q9H8M9; Q5J8X5: MS4A13; NbExp=3; IntAct=EBI-715362, EBI-12070086;
CC Q9H8M9; Q96EQ0: SGTB; NbExp=3; IntAct=EBI-715362, EBI-744081;
CC Q9H8M9; O14817: TSPAN4; NbExp=3; IntAct=EBI-715362, EBI-8652667;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:17492404}; Single-pass membrane protein
CC {ECO:0000269|PubMed:17492404}. Lysosome membrane
CC {ECO:0000269|PubMed:17492404}; Single-pass membrane protein
CC {ECO:0000269|PubMed:17492404}.
CC -!- TISSUE SPECIFICITY: Expressed in lung, kidney, liver, pancreas,
CC placenta, but not in heart and skeletal muscle.
CC {ECO:0000269|PubMed:17492404}.
CC -!- SIMILARITY: Belongs to the EVA1 family. {ECO:0000305}.
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DR EMBL; AK023453; BAB14580.1; -; mRNA.
DR EMBL; AF177337; AAG17981.1; -; mRNA.
DR EMBL; CR457314; CAG33595.1; -; mRNA.
DR EMBL; AC007099; AAY14774.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99591.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99592.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99593.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99594.1; -; Genomic_DNA.
DR EMBL; BC016157; AAH16157.1; -; mRNA.
DR EMBL; BC063016; AAH63016.1; -; mRNA.
DR CCDS; CCDS1959.1; -.
DR RefSeq; NP_001128504.1; NM_001135032.1.
DR RefSeq; NP_115557.1; NM_032181.2.
DR AlphaFoldDB; Q9H8M9; -.
DR BioGRID; 123910; 12.
DR IntAct; Q9H8M9; 9.
DR MINT; Q9H8M9; -.
DR STRING; 9606.ENSP00000233712; -.
DR TCDB; 9.A.15.2.1; the autophagy-related phagophore-formation transporter (apt) family.
DR iPTMnet; Q9H8M9; -.
DR PhosphoSitePlus; Q9H8M9; -.
DR BioMuta; EVA1A; -.
DR DMDM; 74733858; -.
DR EPD; Q9H8M9; -.
DR jPOST; Q9H8M9; -.
DR MassIVE; Q9H8M9; -.
DR MaxQB; Q9H8M9; -.
DR PaxDb; Q9H8M9; -.
DR PeptideAtlas; Q9H8M9; -.
DR PRIDE; Q9H8M9; -.
DR ProteomicsDB; 81229; -.
DR Antibodypedia; 2359; 138 antibodies from 21 providers.
DR DNASU; 84141; -.
DR Ensembl; ENST00000233712.5; ENSP00000233712.1; ENSG00000115363.14.
DR Ensembl; ENST00000393913.8; ENSP00000377490.3; ENSG00000115363.14.
DR Ensembl; ENST00000410071.5; ENSP00000386930.1; ENSG00000115363.14.
DR Ensembl; ENST00000410113.5; ENSP00000386435.1; ENSG00000115363.14.
DR GeneID; 84141; -.
DR KEGG; hsa:84141; -.
DR MANE-Select; ENST00000393913.8; ENSP00000377490.3; NM_001135032.2; NP_001128504.1.
DR UCSC; uc002sni.3; human.
DR CTD; 84141; -.
DR DisGeNET; 84141; -.
DR GeneCards; EVA1A; -.
DR HGNC; HGNC:25816; EVA1A.
DR HPA; ENSG00000115363; Tissue enriched (liver).
DR MIM; 618990; gene.
DR neXtProt; NX_Q9H8M9; -.
DR OpenTargets; ENSG00000115363; -.
DR PharmGKB; PA162387354; -.
DR VEuPathDB; HostDB:ENSG00000115363; -.
DR eggNOG; ENOG502S091; Eukaryota.
DR GeneTree; ENSGT00940000154096; -.
DR InParanoid; Q9H8M9; -.
DR OMA; CHTDCRQ; -.
DR OrthoDB; 1277536at2759; -.
DR PhylomeDB; Q9H8M9; -.
DR TreeFam; TF352986; -.
DR PathwayCommons; Q9H8M9; -.
DR Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-HSA-8957275; Post-translational protein phosphorylation.
DR SignaLink; Q9H8M9; -.
DR BioGRID-ORCS; 84141; 15 hits in 1067 CRISPR screens.
DR ChiTaRS; EVA1A; human.
DR GenomeRNAi; 84141; -.
DR Pharos; Q9H8M9; Tbio.
DR PRO; PR:Q9H8M9; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9H8M9; protein.
DR Bgee; ENSG00000115363; Expressed in right lobe of liver and 107 other tissues.
DR ExpressionAtlas; Q9H8M9; baseline and differential.
DR Genevisible; Q9H8M9; HS.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR InterPro; IPR039500; EVA1_dom.
DR InterPro; IPR039501; EVA1A.
DR PANTHER; PTHR46780:SF14; PTHR46780:SF14; 1.
DR Pfam; PF14851; FAM176; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Autophagy; Endoplasmic reticulum; Lysosome; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..152
FT /note="Protein eva-1 homolog A"
FT /id="PRO_0000278671"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..60
FT /note="Necessary for the localization and biological
FT activity"
FT REGION 70..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 106
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q91WM6"
FT MOD_RES 114
FT /note="Phosphoserine; by FAM20C"
FT /evidence="ECO:0000269|PubMed:26091039"
FT VARIANT 150
FT /note="R -> H (in dbSNP:rs11126472)"
FT /id="VAR_054077"
FT CONFLICT 37
FT /note="Y -> C (in Ref. 2; AAG17981)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 152 AA; 17470 MW; 5ED1FD97D73E801A CRC64;
MRLPLSHSPE HVEMALLSNI LAAYSFVSEN PERAALYFVS GVCIGLVLTL AALVIRISCH
TDCRRRPGKK FLQDRESSSD SSDSEDGSED TVSDLSVRRH RRFERTLNKN VFTSAEELER
AQRLEERERI IREIWMNGQP EVPGTRSLNR YY
