EVE_DROME
ID EVE_DROME Reviewed; 376 AA.
AC P06602; P07667; Q9V5E6;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 3.
DT 03-AUG-2022, entry version 191.
DE RecName: Full=Segmentation protein even-skipped;
GN Name=eve; ORFNames=CG2328;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2877745; DOI=10.1016/0092-8674(86)90515-5;
RA McDonald P.M., Ingham P., Struhl G.;
RT "Isolation, structure, and expression of even-skipped: a second pair-rule
RT gene of Drosophila containing a homeo box.";
RL Cell 47:721-734(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2884106; DOI=10.1002/j.1460-2075.1987.tb04817.x;
RA Frasch M., Hoey T., Rushlow C., Doyle H., Levine M.;
RT "Characterization and localization of the even-skipped protein of
RT Drosophila.";
RL EMBO J. 6:749-759(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
RC STRAIN=AF-S, FL-S, Oregon-RC, WA-F, and ZM56;
RX PubMed=8524036; DOI=10.1093/oxfordjournals.molbev.a040277;
RA Ludwig M.Z., Kreitman M.;
RT "Evolutionary dynamics of the enhancer region of even-skipped in
RT Drosophila.";
RL Mol. Biol. Evol. 12:1002-1011(1995).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 70-189.
RX PubMed=8557047; DOI=10.1002/j.1460-2075.1995.tb00318.x;
RA Hirsch J.A., Aggarwal A.K.;
RT "Structure of the even-skipped homeodomain complexed to AT-rich DNA: new
RT perspectives on homeodomain specificity.";
RL EMBO J. 14:6280-6291(1995).
CC -!- FUNCTION: May play a role in determining neuronal identity. May be
CC directly involved in specifying identity of individual neurons. Pair-
CC rule protein required for segmentation; involved in transforming the
CC broad, spatial, aperiodic expression patterns of the gap genes into a
CC system of precise periodic expression patterns of the pair-rule and
CC segmentary polarity genes.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the even-skipped homeobox family. {ECO:0000305}.
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DR EMBL; M14767; AAA28522.1; -; Genomic_DNA.
DR EMBL; X05138; CAA28784.1; -; mRNA.
DR EMBL; AE013599; AAF58865.1; -; Genomic_DNA.
DR EMBL; U32087; AAB05358.1; -; Genomic_DNA.
DR EMBL; U32088; AAB05359.1; -; Genomic_DNA.
DR EMBL; U32089; AAB05360.1; -; Genomic_DNA.
DR EMBL; U32090; AAB05361.1; -; Genomic_DNA.
DR EMBL; U32091; AAB05362.1; -; Genomic_DNA.
DR PIR; A26066; A26066.
DR RefSeq; NP_523670.2; NM_078946.4.
DR PDB; 1JGG; X-ray; 2.00 A; A/B=70-129.
DR PDBsum; 1JGG; -.
DR AlphaFoldDB; P06602; -.
DR SMR; P06602; -.
DR BioGRID; 61867; 23.
DR IntAct; P06602; 2.
DR STRING; 7227.FBpp0087478; -.
DR PaxDb; P06602; -.
DR DNASU; 36039; -.
DR EnsemblMetazoa; FBtr0088390; FBpp0087478; FBgn0000606.
DR GeneID; 36039; -.
DR KEGG; dme:Dmel_CG2328; -.
DR CTD; 36039; -.
DR FlyBase; FBgn0000606; eve.
DR VEuPathDB; VectorBase:FBgn0000606; -.
DR eggNOG; KOG0844; Eukaryota.
DR HOGENOM; CLU_065032_0_0_1; -.
DR InParanoid; P06602; -.
DR OMA; PAGPHMH; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; P06602; -.
DR SignaLink; P06602; -.
DR BioGRID-ORCS; 36039; 0 hits in 3 CRISPR screens.
DR EvolutionaryTrace; P06602; -.
DR GenomeRNAi; 36039; -.
DR PRO; PR:P06602; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0000606; Expressed in embryonic/larval neuron (Drosophila) and 17 other tissues.
DR ExpressionAtlas; P06602; baseline and differential.
DR Genevisible; P06602; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0007512; P:adult heart development; IMP:FlyBase.
DR GO; GO:0007350; P:blastoderm segmentation; TAS:FlyBase.
DR GO; GO:0007376; P:cephalic furrow formation; IMP:FlyBase.
DR GO; GO:0007377; P:germ-band extension; IMP:FlyBase.
DR GO; GO:0003007; P:heart morphogenesis; IMP:FlyBase.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:FlyBase.
DR GO; GO:0009997; P:negative regulation of cardioblast cell fate specification; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; TAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEP:FlyBase.
DR GO; GO:0035289; P:posterior head segmentation; TAS:FlyBase.
DR GO; GO:0050770; P:regulation of axonogenesis; IMP:FlyBase.
DR GO; GO:1901739; P:regulation of myoblast fusion; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035290; P:trunk segmentation; TAS:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Pair-rule protein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..376
FT /note="Segmentation protein even-skipped"
FT /id="PRO_0000049060"
FT DNA_BIND 70..129
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 281..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 300
FT /note="L -> V (in Ref. 1; AAA28522)"
FT /evidence="ECO:0000305"
FT HELIX 79..91
FT /evidence="ECO:0007829|PDB:1JGG"
FT HELIX 97..107
FT /evidence="ECO:0007829|PDB:1JGG"
FT HELIX 111..127
FT /evidence="ECO:0007829|PDB:1JGG"
SQ SEQUENCE 376 AA; 39970 MW; 59058E0634B1BED0 CRC64;
MHGYRTYNME SHHAHHDASP VDQKPLVVDL LATQYGKPQT PPPSPNECLS SPDNSLNGSR
GSEIPADPSV RRYRTAFTRD QLGRLEKEFY KENYVSRPRR CELAAQLNLP ESTIKVWFQN
RRMKDKRQRI AVAWPYAAVY SDPAFAASIL QAAANSVGMP YPPYAPAAAA AAAAAAAVAT
NPMMATGMPP MGMPQMPTMQ MPGHSGHAGH PSPYGQYRYT PYHIPARPAP PHPAGPHMHH
PHMMGSSATG SSYSAGAAGL LGALPSATCY TGLGVGVPKT QTPPLDLQSS SSPHSSTLSL
SPVGSDHAKV FDRSPVAQSA PSVPAPAPLT TTSPLPAPGL LMPSAKRPAS DMSPPPTTTV
IAEPKPKLFK PYKTEA
