EVGA_ECOLI
ID EVGA_ECOLI Reviewed; 204 AA.
AC P0ACZ4; P30854;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=DNA-binding transcriptional activator EvgA {ECO:0000305};
GN Name=evgA; OrderedLocusNames=b2369, JW2366;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8125343; DOI=10.1016/0378-1119(94)90733-1;
RA Utsumi R., Katayama S., Taniguchi M., Horie T., Ikeda M., Igaki S.,
RA Nakagawa H., Miwa A., Tanabe H., Noda M.;
RT "Newly identified genes involved in the signal transduction of Escherichia
RT coli K-12.";
RL Gene 140:73-77(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1289796;
RA Utsumi R., Katayama S., Ikeda M., Igaki S., Nakagawa H., Miwa A.,
RA Taniguchi M., Noda M.;
RT "Cloning and sequence analysis of the evgAS genes involved in signal
RT transduction of Escherichia coli K-12.";
RL Nucleic Acids Symp. Ser. 27:149-150(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP CHARACTERIZATION.
RX PubMed=9535079; DOI=10.1046/j.1365-2958.1998.00716.x;
RA Perraud A.-L., Kimmel B., Weiss V., Gross R.;
RT "Specificity of the BvgAS and EvgAS phosphorelay is mediated by the C-
RT terminal HPt domains of the sensor proteins.";
RL Mol. Microbiol. 27:875-887(1998).
RN [7]
RP CHARACTERIZATION, AND MASS SPECTROMETRY.
RX PubMed=10825546; DOI=10.1016/s0167-4838(00)00052-2;
RA Perraud A.-L., Rippe K., Bantscheff M., Glocker M., Lucassen M., Jung K.,
RA Sebald W., Weiss V., Gross R.;
RT "Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay
RT systems.";
RL Biochim. Biophys. Acta 1478:341-354(2000).
RN [8]
RP CHARACTERIZATION.
RC STRAIN=K12;
RX PubMed=10923791; DOI=10.1271/bbb.64.1203;
RA Kato A., Ohnishi H., Yamamoto K., Furuta E., Tanabe H., Utsumi R.;
RT "Transcription of emrKY is regulated by the EvgA-EvgS two-component system
RT in Escherichia coli K-12.";
RL Biosci. Biotechnol. Biochem. 64:1203-1209(2000).
RN [9]
RP CHARACTERIZATION.
RC STRAIN=K12 / W3104 / ATCC 19020;
RX PubMed=11157960; DOI=10.1128/jb.183.4.1455-1458.2001;
RA Nishino K., Yamaguchi A.;
RT "Overexpression of the response regulator evgA of the two-component signal
RT transduction system modulates multidrug resistance conferred by multidrug
RT resistance transporters.";
RL J. Bacteriol. 183:1455-1458(2001).
CC -!- FUNCTION: Member of the two-component regulatory system EvgS/EvgA.
CC Regulates the expression of emrKY operon and yfdX. Seems also to
CC control expression of at least one other multidrug efflux operon.
CC -!- SUBUNIT: Homodimer.
CC -!- INTERACTION:
CC P0ACZ4; P0AFB8: glnG; NbExp=3; IntAct=EBI-548694, EBI-1113197;
CC P0ACZ4; P45577: proQ; NbExp=3; IntAct=EBI-548694, EBI-8767901;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by EvgS.
CC -!- MASS SPECTROMETRY: Mass=22693; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10825546};
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DR EMBL; D14008; BAA03107.1; -; Genomic_DNA.
DR EMBL; D11142; BAA01918.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75428.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16240.1; -; Genomic_DNA.
DR PIR; JU0220; JU0220.
DR RefSeq; NP_416870.1; NC_000913.3.
DR RefSeq; WP_000991370.1; NZ_STEB01000008.1.
DR PDB; 3F6C; X-ray; 1.45 A; A/B=2-133.
DR PDBsum; 3F6C; -.
DR AlphaFoldDB; P0ACZ4; -.
DR SMR; P0ACZ4; -.
DR BioGRID; 4260558; 15.
DR BioGRID; 851182; 9.
DR DIP; DIP-48087N; -.
DR IntAct; P0ACZ4; 15.
DR STRING; 511145.b2369; -.
DR iPTMnet; P0ACZ4; -.
DR jPOST; P0ACZ4; -.
DR PaxDb; P0ACZ4; -.
DR PRIDE; P0ACZ4; -.
DR DNASU; 946841; -.
DR EnsemblBacteria; AAC75428; AAC75428; b2369.
DR EnsemblBacteria; BAA16240; BAA16240; BAA16240.
DR GeneID; 66673761; -.
DR GeneID; 946841; -.
DR KEGG; ecj:JW2366; -.
DR KEGG; eco:b2369; -.
DR PATRIC; fig|1411691.4.peg.4360; -.
DR EchoBASE; EB1566; -.
DR eggNOG; COG2197; Bacteria.
DR HOGENOM; CLU_000445_90_1_6; -.
DR InParanoid; P0ACZ4; -.
DR OMA; YKKRLMQ; -.
DR PhylomeDB; P0ACZ4; -.
DR BioCyc; EcoCyc:EVGA-MON; -.
DR EvolutionaryTrace; P0ACZ4; -.
DR PRO; PR:P0ACZ4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; DNA-binding; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..204
FT /note="DNA-binding transcriptional activator EvgA"
FT /id="PRO_0000081035"
FT DOMAIN 2..117
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 137..202
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 161..180
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 52
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 10..22
FT /evidence="ECO:0007829|PDB:3F6C"
FT STRAND 25..34
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 37..44
FT /evidence="ECO:0007829|PDB:3F6C"
FT STRAND 47..52
FT /evidence="ECO:0007829|PDB:3F6C"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 60..69
FT /evidence="ECO:0007829|PDB:3F6C"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 88..93
FT /evidence="ECO:0007829|PDB:3F6C"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 107..117
FT /evidence="ECO:0007829|PDB:3F6C"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:3F6C"
SQ SEQUENCE 204 AA; 22690 MW; E68F932F3729FBBB CRC64;
MNAIIIDDHP LAIAAIRNLL IKNDIEILAE LTEGGSAVQR VETLKPDIVI IDVDIPGVNG
IQVLETLRKR QYSGIIIIVS AKNDHFYGKH CADAGANGFV SKKEGMNNII AAIEAAKNGY
CYFPFSLNRF VGSLTSDQQK LDSLSKQEIS VMRYILDGKD NNDIAEKMFI SNKTVSTYKS
RLMEKLECKS LMDLYTFAQR NKIG
