EVI2B_HUMAN
ID EVI2B_HUMAN Reviewed; 448 AA.
AC P34910; B7Z4A7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Protein EVI2B {ECO:0000305};
DE AltName: Full=Ecotropic viral integration site 2B protein homolog;
DE Short=EVI-2B {ECO:0000303|PubMed:1903357};
DE AltName: CD_antigen=CD361;
DE Flags: Precursor;
GN Name=EVI2B {ECO:0000303|PubMed:1903357, ECO:0000312|HGNC:HGNC:3500};
GN Synonyms=EVDB {ECO:0000312|HGNC:HGNC:3500};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RC TISSUE=Bone marrow;
RX PubMed=1903357; DOI=10.1016/0888-7543(91)90410-g;
RA Cawthon R.M., Andersen L.B., Buchberg A.M., Xu G., O'Connell P.,
RA Viskochil D., Weiss R.B., Wallace M.R., Marchuk D.A., Culver M.,
RA Stevens J., Jenkins N.A., Copeland N.G., Collins F.S., White R.;
RT "cDNA sequence and genomic structure of EVI2B, a gene lying within an
RT intron of the neurofibromatosis type 1 gene.";
RL Genomics 9:446-460(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Umbilical cord blood;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268; SER-271 AND SER-294, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION, AND FUNCTION.
RX PubMed=28186500; DOI=10.1038/cdd.2017.6;
RA Zjablovskaja P., Kardosova M., Danek P., Angelisova P., Benoukraf T.,
RA Wurm A.A., Kalina T., Sian S., Balastik M., Delwel R., Brdicka T.,
RA Tenen D.G., Behre G., Fiore F., Malissen B., Horejsi V., Alberich-Jorda M.;
RT "EVI2B is a C/EBPalpha target gene required for granulocytic
RT differentiation and functionality of hematopoietic progenitors.";
RL Cell Death Differ. 24:705-716(2017).
CC -!- FUNCTION: Required for granulocyte differentiation and functionality of
CC hematopoietic progenitor cells through the control of cell cycle
CC progression and survival of hematopoietic progenitor cells.
CC {ECO:0000269|PubMed:28186500}.
CC -!- INTERACTION:
CC P34910-2; P05090: APOD; NbExp=3; IntAct=EBI-17640610, EBI-715495;
CC P34910-2; P29972: AQP1; NbExp=3; IntAct=EBI-17640610, EBI-745213;
CC P34910-2; Q92482: AQP3; NbExp=3; IntAct=EBI-17640610, EBI-2808854;
CC P34910-2; Q8WVV5: BTN2A2; NbExp=3; IntAct=EBI-17640610, EBI-8648738;
CC P34910-2; O14735: CDIPT; NbExp=3; IntAct=EBI-17640610, EBI-358858;
CC P34910-2; Q9NWW5: CLN6; NbExp=3; IntAct=EBI-17640610, EBI-6165897;
CC P34910-2; O95406: CNIH1; NbExp=3; IntAct=EBI-17640610, EBI-12172273;
CC P34910-2; Q6ICB0: DESI1; NbExp=3; IntAct=EBI-17640610, EBI-2806959;
CC P34910-2; Q8N682: DRAM1; NbExp=3; IntAct=EBI-17640610, EBI-10305400;
CC P34910-2; Q92520: FAM3C; NbExp=3; IntAct=EBI-17640610, EBI-2876774;
CC P34910-2; Q9BWH2: FUNDC2; NbExp=3; IntAct=EBI-17640610, EBI-714482;
CC P34910-2; Q8N3T1: GALNT15; NbExp=3; IntAct=EBI-17640610, EBI-3925203;
CC P34910-2; Q96F15: GIMAP5; NbExp=3; IntAct=EBI-17640610, EBI-6166686;
CC P34910-2; A6NDP7: MYADML2; NbExp=3; IntAct=EBI-17640610, EBI-17641390;
CC P34910-2; Q9NZG7: NINJ2; NbExp=3; IntAct=EBI-17640610, EBI-10317425;
CC P34910-2; P42857: NSG1; NbExp=3; IntAct=EBI-17640610, EBI-6380741;
CC P34910-2; O00264: PGRMC1; NbExp=3; IntAct=EBI-17640610, EBI-1045534;
CC P34910-2; Q5QGT7: RTP2; NbExp=3; IntAct=EBI-17640610, EBI-10244780;
CC P34910-2; Q969E2: SCAMP4; NbExp=3; IntAct=EBI-17640610, EBI-4403649;
CC P34910-2; P22732: SLC2A5; NbExp=3; IntAct=EBI-17640610, EBI-2825135;
CC P34910-2; Q9BRI3: SLC30A2; NbExp=3; IntAct=EBI-17640610, EBI-8644112;
CC P34910-2; Q9NVC3: SLC38A7; NbExp=3; IntAct=EBI-17640610, EBI-10314552;
CC P34910-2; Q9NUM3: SLC39A9; NbExp=3; IntAct=EBI-17640610, EBI-2823239;
CC P34910-2; Q6UX40: TMEM107; NbExp=3; IntAct=EBI-17640610, EBI-12845616;
CC P34910-2; Q9H0R3: TMEM222; NbExp=3; IntAct=EBI-17640610, EBI-347385;
CC P34910-2; Q9H2L4: TMEM60; NbExp=3; IntAct=EBI-17640610, EBI-2852148;
CC P34910-2; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-17640610, EBI-2548832;
CC P34910-2; Q9Y228: TRAF3IP3; NbExp=3; IntAct=EBI-17640610, EBI-765817;
CC P34910-2; Q8N609: TRAM1L1; NbExp=3; IntAct=EBI-17640610, EBI-11996766;
CC P34910-2; Q9Y5Z9: UBIAD1; NbExp=3; IntAct=EBI-17640610, EBI-2819725;
CC P34910-2; O75379: VAMP4; NbExp=3; IntAct=EBI-17640610, EBI-744953;
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P34910-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P34910-2; Sequence=VSP_056461;
CC -!- TISSUE SPECIFICITY: Bone marrow, peripheral blood mononuclear cells,
CC fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell
CC lines. Strongly expressed in granulocytic cells, and weakly on
CC lymphocytes cells. {ECO:0000269|PubMed:28186500}.
CC -!- INDUCTION: Up-regulated by full-length CEBPA.
CC {ECO:0000269|PubMed:28186500}.
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DR EMBL; M60830; AAA35816.1; -; Genomic_DNA.
DR EMBL; AK297087; BAH12493.1; -; mRNA.
DR EMBL; AC134669; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS11266.1; -. [P34910-1]
DR PIR; A38445; A38445.
DR RefSeq; NP_006486.3; NM_006495.3. [P34910-1]
DR RefSeq; XP_005258003.1; XM_005257946.3. [P34910-2]
DR AlphaFoldDB; P34910; -.
DR BioGRID; 108425; 33.
DR IntAct; P34910; 32.
DR STRING; 9606.ENSP00000333779; -.
DR GlyGen; P34910; 5 sites, 2 O-linked glycans (2 sites).
DR iPTMnet; P34910; -.
DR PhosphoSitePlus; P34910; -.
DR SwissPalm; P34910; -.
DR BioMuta; EVI2B; -.
DR DMDM; 281185464; -.
DR CPTAC; CPTAC-1182; -.
DR CPTAC; CPTAC-1207; -.
DR EPD; P34910; -.
DR jPOST; P34910; -.
DR MassIVE; P34910; -.
DR MaxQB; P34910; -.
DR PaxDb; P34910; -.
DR PeptideAtlas; P34910; -.
DR PRIDE; P34910; -.
DR ProteomicsDB; 54952; -. [P34910-1]
DR ProteomicsDB; 6585; -.
DR Antibodypedia; 1927; 211 antibodies from 27 providers.
DR DNASU; 2124; -.
DR Ensembl; ENST00000330927.5; ENSP00000333779.4; ENSG00000185862.7. [P34910-1]
DR Ensembl; ENST00000577894.1; ENSP00000462682.1; ENSG00000185862.7. [P34910-1]
DR GeneID; 2124; -.
DR KEGG; hsa:2124; -.
DR MANE-Select; ENST00000330927.5; ENSP00000333779.4; NM_006495.4; NP_006486.3.
DR UCSC; uc002hgk.3; human. [P34910-1]
DR CTD; 2124; -.
DR DisGeNET; 2124; -.
DR GeneCards; EVI2B; -.
DR HGNC; HGNC:3500; EVI2B.
DR HPA; ENSG00000185862; Tissue enhanced (bone marrow, lymphoid tissue).
DR MIM; 158381; gene.
DR neXtProt; NX_P34910; -.
DR OpenTargets; ENSG00000185862; -.
DR PharmGKB; PA27914; -.
DR VEuPathDB; HostDB:ENSG00000185862; -.
DR eggNOG; ENOG502S3T8; Eukaryota.
DR GeneTree; ENSGT00390000009142; -.
DR HOGENOM; CLU_048893_0_0_1; -.
DR InParanoid; P34910; -.
DR OMA; NNEIQCQ; -.
DR OrthoDB; 997203at2759; -.
DR PhylomeDB; P34910; -.
DR TreeFam; TF336167; -.
DR PathwayCommons; P34910; -.
DR SignaLink; P34910; -.
DR BioGRID-ORCS; 2124; 4 hits in 1063 CRISPR screens.
DR ChiTaRS; EVI2B; human.
DR GeneWiki; EVI2B; -.
DR GenomeRNAi; 2124; -.
DR Pharos; P34910; Tbio.
DR PRO; PR:P34910; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; P34910; protein.
DR Bgee; ENSG00000185862; Expressed in monocyte and 174 other tissues.
DR Genevisible; P34910; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0061515; P:myeloid cell development; ISS:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0030854; P:positive regulation of granulocyte differentiation; IMP:UniProtKB.
DR GO; GO:0045660; P:positive regulation of neutrophil differentiation; ISS:UniProtKB.
DR GO; GO:0051726; P:regulation of cell cycle; ISS:UniProtKB.
DR GO; GO:2000035; P:regulation of stem cell division; ISS:UniProtKB.
DR InterPro; IPR033239; EVI2B.
DR PANTHER; PTHR15384; PTHR15384; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Membrane; Phosphoprotein;
KW Proto-oncogene; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..448
FT /note="Protein EVI2B"
FT /id="PRO_0000021213"
FT TOPO_DOM 22..202
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 74..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..315
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 316..332
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 249
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD58"
FT MOD_RES 268
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 271
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 278
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD58"
FT MOD_RES 294
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CARBOHYD 16
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1
FT /note="M -> MLFRYAEKITRKYSEM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056461"
FT VARIANT 53
FT /note="G -> R (in dbSNP:rs9903564)"
FT /id="VAR_056871"
FT CONFLICT 37
FT /note="F -> Y (in Ref. 1; AAA35816)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 48666 MW; 5BF2DC46798577E3 CRC64;
MDPKYFILIL FCGHLNNTFF SKTETITTEK QSQPTLFTSS MSQVLANSQN TTGNPLGQPT
QFSDTFSGQS ISPAKVTAGQ PTPAVYTSSE KPEAHTSAGQ PLAYNTKQPT PIANTSSQQA
VFTSARQLPS ARTSTTQPPK SFVYTFTQQS SSVQIPSRKQ ITVHNPSTQP TSTVKNSPRS
TPGFILDTTS NKQTPQKNNY NSIAAILIGV LLTSMLVAII IIVLWKCLRK PVLNDQNWAG
RSPFADGETP DICMDNIREN EISTKRTSII SLTPWKPSKS TLLADDLEIK LFESSENIED
SNNPKTEKIK DQVNGTSEDS ADGSTVGTAV SSSDDADLPP PPPLLDLEGQ ESNQSDKPTM
TIVSPLPNDS TSLPPSLDCL NQDCGDHKSE IIQSFPPLDS LNLPLPPVDF MKNQEDSNLE
IQCQEFSIPP NSDQDLNESL PPPPAELL
