EX53_BACSU
ID EX53_BACSU Reviewed; 296 AA.
AC P54161;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=5'-3' exonuclease;
DE EC=3.1.11.-;
GN Name=ypcP; OrderedLocusNames=BSU22010;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8969496; DOI=10.1099/13500872-142-11-3005;
RA Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S.D.;
RT "Organization of the Bacillus subtilis 168 chromosome between kdg and the
RT attachment site of the SP beta prophage: use of long accurate PCR and yeast
RT artificial chromosomes for sequencing.";
RL Microbiology 142:3005-3015(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION AS AN EXONUCLEASE.
RX PubMed=17114254; DOI=10.1128/jb.01381-06;
RA Thomaides H.B., Davison E.J., Burston L., Johnson H., Brown D.R.,
RA Hunt A.C., Errington J., Czaplewski L.;
RT "Essential bacterial functions encoded by gene pairs.";
RL J. Bacteriol. 189:591-602(2007).
RN [4]
RP FUNCTION AS AN EXONUCLEASE.
RC STRAIN=168;
RX PubMed=17905985; DOI=10.1128/jb.00653-07;
RA Fukushima S., Itaya M., Kato H., Ogasawara N., Yoshikawa H.;
RT "Reassessment of the in vivo functions of DNA polymerase I and RNase H in
RT bacterial cell growth.";
RL J. Bacteriol. 189:8575-8583(2007).
CC -!- FUNCTION: 5'-3' exonuclease acting preferentially on double-stranded
CC DNA. {ECO:0000269|PubMed:17114254, ECO:0000269|PubMed:17905985}.
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DR EMBL; L77246; AAA96617.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14118.1; -; Genomic_DNA.
DR PIR; H69933; H69933.
DR RefSeq; NP_390083.1; NC_000964.3.
DR RefSeq; WP_003230763.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P54161; -.
DR SMR; P54161; -.
DR STRING; 224308.BSU22010; -.
DR PaxDb; P54161; -.
DR PRIDE; P54161; -.
DR EnsemblBacteria; CAB14118; CAB14118; BSU_22010.
DR GeneID; 939070; -.
DR KEGG; bsu:BSU22010; -.
DR PATRIC; fig|224308.179.peg.2403; -.
DR eggNOG; COG0258; Bacteria.
DR InParanoid; P54161; -.
DR OMA; WRVDLIP; -.
DR PhylomeDB; P54161; -.
DR BioCyc; BSUB:BSU22010-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:InterPro.
DR CDD; cd09898; H3TH_53EXO; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR038969; FEN.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR PANTHER; PTHR42646; PTHR42646; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT CHAIN 1..296
FT /note="5'-3' exonuclease"
FT /id="PRO_0000101282"
FT DOMAIN 175..262
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000255"
SQ SEQUENCE 296 AA; 32944 MW; 67958BEC67F970B3 CRC64;
MNNNKLLLVD GMALLFRAFF ATAVHRNFMI NDSGVPTNGV NGFLKHLITA VETFQPTHVV
CCWDMGSKTY RNDLFQDYKA NRSAPPVELI PQFDLAKEAA AELGIMNIGF AGYEADDCIG
TLADLFANEA DITVVTGDRD LLQLLTDKVS VALLQKGIGN YKVYTKETFY EETGVMPKAL
IDIKALMGDS SDNYPGVKGI GEKTAYKLIR EYETIDRLLE NLSLLPKGQQ GKIQQGLSDL
EMSRKLAEIH CSVPLACTLK DALFTLQMEQ AADMLRRHQI KGIERMLEKL NAREIV
