ID   EX53_BUCAI              Reviewed;         286 AA.
AC   P57506;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=5'-3' exonuclease;
DE            EC=3.1.11.-;
GN   OrderedLocusNames=BU431;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: 5'-3' exonuclease acting preferentially on double-stranded
CC       DNA. {ECO:0000250}.
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DR   EMBL; BA000003; BAB13129.1; -; Genomic_DNA.
DR   RefSeq; NP_240243.1; NC_002528.1.
DR   RefSeq; WP_010896113.1; NC_002528.1.
DR   AlphaFoldDB; P57506; -.
DR   SMR; P57506; -.
DR   STRING; 107806.10039095; -.
DR   EnsemblBacteria; BAB13129; BAB13129; BAB13129.
DR   KEGG; buc:BU431; -.
DR   PATRIC; fig|107806.10.peg.440; -.
DR   eggNOG; COG0258; Bacteria.
DR   HOGENOM; CLU_004675_1_0_6; -.
DR   OMA; WRVDLIP; -.
DR   BioCyc; BAPH107806:GBZJ-424-MON; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:InterPro.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR038969; FEN.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   PANTHER; PTHR42646; PTHR42646; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..286
FT                   /note="5'-3' exonuclease"
FT                   /id="PRO_0000101283"
FT   DOMAIN          172..270
FT                   /note="5'-3' exonuclease"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   286 AA;  32982 MW;  D7F730821AD08C58 CRC64;
     MNHIKNNPVI IIDGSLYLYA SYYAFCNLEN TSGKPCGAIY GMLKIIDNIF KKYKNLKKII
     IIFDSSRKTF RNKLFKEYKK NRSSMPDLLV MQIQPLFEIL KKIGIKTLTI PGIEADDVIG
     SLSYQLEKQG EKILILSHDK DMLQLVTENI NIFHKKNNCI ITSEIIQEKY GIKPKEFIDF
     LALMGDATDN IPGVPKIGIK TALFLINKFS NIENIYNNIE KIKSLPLRNA KNISIQLKNN
     KERALLSYKL ARIKLDIPID IKLKDIILKK YCSKNTFQIF KNYFSS