ID   EX53_MYCTU              Reviewed;         318 AA.
AC   P9WNU3; L0TBC4; Q10699;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=5'-3' exonuclease;
DE            EC=3.1.11.-;
GN   OrderedLocusNames=Rv2090; ORFNames=MTCY49.30;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: 5'-3' exonuclease acting preferentially on double-stranded
CC       DNA. {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP44865.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP44865.1; ALT_INIT; Genomic_DNA.
DR   PIR; E70767; E70767.
DR   RefSeq; NP_216606.1; NC_000962.3.
DR   RefSeq; WP_009935270.1; NC_018143.2.
DR   AlphaFoldDB; P9WNU3; -.
DR   SMR; P9WNU3; -.
DR   STRING; 83332.Rv2090; -.
DR   PaxDb; P9WNU3; -.
DR   DNASU; 887924; -.
DR   GeneID; 887924; -.
DR   KEGG; mtu:Rv2090; -.
DR   TubercuList; Rv2090; -.
DR   eggNOG; COG0258; Bacteria.
DR   OMA; WRVDLIP; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:InterPro.
DR   CDD; cd09898; H3TH_53EXO; 1.
DR   InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR   InterPro; IPR002421; 5-3_exonuclease.
DR   InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR   InterPro; IPR020045; DNA_polI_H3TH.
DR   InterPro; IPR038969; FEN.
DR   InterPro; IPR008918; HhH2.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   PANTHER; PTHR42646; PTHR42646; 1.
DR   Pfam; PF01367; 5_3_exonuc; 1.
DR   Pfam; PF02739; 5_3_exonuc_N; 1.
DR   SMART; SM00475; 53EXOc; 1.
DR   SMART; SM00279; HhH2; 1.
DR   SUPFAM; SSF47807; SSF47807; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..318
FT                   /note="5'-3' exonuclease"
FT                   /id="PRO_0000101290"
FT   DOMAIN          194..278
FT                   /note="5'-3' exonuclease"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   318 AA;  34023 MW;  413985E2E2D381A9 CRC64;
     MRSPLVLLDG ASMWFRSFFG VPSSITAPDG RPVNAVRGFI DSMAVVITQQ RPNRLAVCLD
     LDWRPQFRVD LIPSYKAHRV AEPEPNGQPD VEEVPDELTP QVDMIMELLD AFGIAMAGAP
     GFEADDVLGT LATRERRDPV IVVSGDRDLL QVVADDPVPV RVLYLGRGLA KATLFGPAEV
     AERYGLPAHR AGAAYAELAL LRGDPSDGLP GVPGVGEKTA ATLLARHGSL DQIMAAADDR
     KTTMAKGLRT KLLAASAYIK AADRVVRVAT DAPVTLSTPT DRFPLVAADP ERTAELATRF
     GVESSIARLQ KALDTLPG