EX70I_MEDTR
ID EX70I_MEDTR Reviewed; 712 AA.
AC A0A396JG59; G7IBC1;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2018, sequence version 1.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Exocyst complex component EXO70I {ECO:0000303|PubMed:26234213};
DE Short=MtExo70I {ECO:0000303|PubMed:26234213};
DE AltName: Full=Exocyst subunit Exo70 family protein I {ECO:0000303|PubMed:26234213};
DE Flags: Precursor;
GN Name=EX70I {ECO:0000303|PubMed:26234213};
GN OrderedLocusNames=MTR_1g017910 {ECO:0000312|EMBL:AES59305.1};
GN ORFNames=MtrunA17_Chr1g0152401 {ECO:0000312|EMBL:RHN77220.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH VPY,
RP INDUCTION BY ARBUSCULAR MYCORRHIZAL FUNGI, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RX PubMed=26234213; DOI=10.1016/j.cub.2015.06.075;
RA Zhang X., Pumplin N., Ivanov S., Harrison M.J.;
RT "EXO70I is required for development of a sub-domain of the periarbuscular
RT membrane during arbuscular mycorrhizal symbiosis.";
RL Curr. Biol. 25:2189-2195(2015).
RN [5]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Component of an exocyst subcomplex specifically required for
CC periarbuscular membrane (PAM) biogenesis during arbuscular mycorrhizal
CC (AM) symbiosis with AM fungi (e.g. Glomus versiforme), especially
CC critical during the early branching phase of arbuscule development;
CC probably involved in STR and STR2 delivery into the PAM.
CC {ECO:0000269|PubMed:26234213}.
CC -!- SUBUNIT: Subunit of the exocyst complex that mediates vesicle tethering
CC during exocytosis (Probable). Interacts with VPY at the periarbuscular
CC membrane (PAM) around the arbuscule hyphal tips (PubMed:26234213).
CC {ECO:0000269|PubMed:26234213, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26234213};
CC Peripheral membrane protein {ECO:0000305}; Cytoplasmic side
CC {ECO:0000305}. Note=During arbuscule branching, restricted to zones
CC adjacent to the periarbuscular membrane (PAM) around the arbuscule
CC hyphal tips. {ECO:0000269|PubMed:26234213}.
CC -!- TISSUE SPECIFICITY: Present at low levels in non-mycorrhizal root tips.
CC {ECO:0000269|PubMed:26234213}.
CC -!- DEVELOPMENTAL STAGE: During arbuscular mycorrhizal (AM) symbiosis with
CC (AM) fungi (e.g. Glomus versiforme), accumulates strongly in cortical
CC cells containing arbuscules. {ECO:0000269|PubMed:26234213}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal fungi (e.g. Glomus versiforme)
CC (PubMed:26511916, PubMed:26234213). Triggered by RAM1
CC (PubMed:26511916). Induced during root nodule symbiosis with rhizobium
CC bacteria (e.g. Sinorhizobium meliloti) (PubMed:26234213).
CC {ECO:0000269|PubMed:26234213, ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: Abnormal arbuscule development (reduced arbuscule
CC branching and aberrant hyphal branches frequently distorted in shape)
CC during arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus
CC versiforme) characterized by reduced STR and STR2 incorporation into
CC the periarbuscular membrane (PAM) (PubMed:26234213). Normal root
CC nodules development during symbiosis with rhizobium bacteria (e.g.
CC Sinorhizobium meliloti) (PubMed:26234213).
CC {ECO:0000269|PubMed:26234213}.
CC -!- SIMILARITY: Belongs to the EXO70 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AES59305.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CM001217; AES59305.1; ALT_INIT; Genomic_DNA.
DR EMBL; PSQE01000001; RHN77220.1; -; Genomic_DNA.
DR RefSeq; XP_003589054.1; XM_003589006.1.
DR AlphaFoldDB; A0A396JG59; -.
DR SMR; A0A396JG59; -.
DR STRING; 3880.AES59305; -.
DR EnsemblPlants; AES59305; AES59305; MTR_1g017910.
DR GeneID; 11416358; -.
DR Gramene; AES59305; AES59305; MTR_1g017910.
DR KEGG; mtr:MTR_1g017910; -.
DR eggNOG; KOG2344; Eukaryota.
DR HOGENOM; CLU_025295_0_0_1; -.
DR OrthoDB; 410847at2759; -.
DR Proteomes; UP000002051; Chromosome 1.
DR Proteomes; UP000265566; Chromosome 1.
DR GO; GO:0000145; C:exocyst; IEA:InterPro.
DR GO; GO:0085042; C:periarbuscular membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IEA:InterPro.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0009609; P:response to symbiotic bacterium; IEP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR InterPro; IPR016159; Cullin_repeat-like_dom_sf.
DR InterPro; IPR004140; Exo70.
DR InterPro; IPR046364; Exo70_C.
DR PANTHER; PTHR12542; PTHR12542; 1.
DR Pfam; PF03081; Exo70; 1.
DR SUPFAM; SSF74788; SSF74788; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; Exocytosis; Glycoprotein; Membrane;
KW Protein transport; Reference proteome; Signal; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..712
FT /note="Exocyst complex component EXO70I"
FT /id="PRO_0000450040"
FT COILED 26..53
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 712 AA; 82325 MW; 6A7732CD6ABC68E6 CRC64;
MHKKQLMALL MVPQTSDSQD ATITKLESAY SDLESLLRSS KQMEQNIETM ETRFDLLHGS
ITTASRRVHP LQSLSMSRKA LDTRINRAIS PALALLETFK LAESLQNNLL NLSSKLSTEK
THQKRLSKLL DYMDCVDQLN EAINSISEVV EPVIMRLQEV VEFISRTKAA DQYRTQRLRE
ALITLKALYE TEVDEMRFEG LLDQALLHMQ DEFEVLLLKL KHRKLGDMSH MQNGGEDCDD
HFEVSFELGS ELEIEVLRRI SNTLAANDCL DICIDIYVKV RYKRAAKALM KLNPDYLRTY
TPEGIDEMEW ENLETSITLW TQHFEVATKK VLLSEKKLCE SVLGEIIDGL IHPECFVKIS
DKIMAVFFRF GEGVARSNKE PQKLFKLLDM FESLEKLKPY VLEIFDGESG EDICARFREL
EKLIIDASSK VFWEFGLQIE GNVDGFLPPP QDGSVPKIVR YAVNYLKYLS TENYRTTMAK
VLRTELTWKT ELMLSSKQSE TDEDLLKHAI CNVMEALQRN IESKRLSCKD KILVNIFMMN
TYWYMYMRTK NTELGDLLGE KYIKESYKAV AEESAYLYQK QAWLVLVKIL DQDDDDIKEQ
KQGKEKSIGR LVNEKIETFF KCLSEICDRH RSFYSIPDVD LREQMRDSTV KLLVPVYAEF
LESYSGFLQR KVYPSPQRLQ GLLGKAFGST NDWNLNGGRN SGSLETDIRR SR
