ID   EX7L_BRUME              Reviewed;         511 AA.
AC   Q8YCK1;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2003, sequence version 2.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE   AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN   Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=BMEII0527;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC       insoluble oligonucleotides, which are then degraded further into small
CC       acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC         direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC   -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00378}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL53769.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE008918; AAL53769.1; ALT_INIT; Genomic_DNA.
DR   PIR; AF3575; AF3575.
DR   RefSeq; WP_004682090.1; NZ_GG703779.1.
DR   AlphaFoldDB; Q8YCK1; -.
DR   SMR; Q8YCK1; -.
DR   STRING; 224914.BMEII0527; -.
DR   PRIDE; Q8YCK1; -.
DR   EnsemblBacteria; AAL53769; AAL53769; BMEII0527.
DR   GeneID; 29595775; -.
DR   KEGG; bme:BMEII0527; -.
DR   PATRIC; fig|224914.52.peg.2846; -.
DR   eggNOG; COG1570; Bacteria.
DR   OMA; LVWPVKV; -.
DR   PhylomeDB; Q8YCK1; -.
DR   PRO; PR:Q8YCK1; -.
DR   Proteomes; UP000000419; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR   GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd04489; ExoVII_LU_OBF; 1.
DR   HAMAP; MF_00378; Exonuc_7_L; 1.
DR   InterPro; IPR003753; Exonuc_VII_L.
DR   InterPro; IPR020579; Exonuc_VII_lsu_C.
DR   InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR   PANTHER; PTHR30008; PTHR30008; 1.
DR   Pfam; PF02601; Exonuc_VII_L; 1.
DR   Pfam; PF13742; tRNA_anti_2; 1.
DR   TIGRFAMs; TIGR00237; xseA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..511
FT                   /note="Exodeoxyribonuclease 7 large subunit"
FT                   /id="PRO_0000197830"
SQ   SEQUENCE   511 AA;  56333 MW;  6760E9944B4600E7 CRC64;
     MASDSSFPGA SSNVAEYSVS EISGALKRTV EDTFGHVRVR GEISGYRGPH SSGHAYFALK
     DDRARLEAVI WRGSMSRLRF RPEEGMEVIA TGKLTTYPGS SKYQIVIEQM EPAGAGALMA
     LLEERKQRLA AEGLFDPALK QLLPFMPRVI GVVTSPTGAV IRDIIHRISD RYPLRVIVWP
     VRVQGDTCGP EVATAVNGFN TLPDDGPIPR PDVLIVARGG GSLEDLWGFN DEIVVRAVAA
     SHIPVISAVG HETDWTLIDL AADMRAPTPT GAAEMAVPVK ADLQASLASQ SARLSSAMSR
     FFDQKRQAHR AAARAMPSAD QLLALPRRRF DEAASRLTRA LFVNTQKKRV HFDGHARQLS
     PRLLQRRLVE LERGVTMLGQ RLPRALEAFL RERRTAFTHR ANRLSPEPIL RRTRLTGSTL
     EQLDRRRDQA VRLLIERVKR RSQELDRLMR TLSYESVLER GFAVVFDAQG KPVKQAAAVS
     PGDALSVRFR DGDVGVVARA GLTIPDPTKG Q