ID   EX7L_CLOD6              Reviewed;         401 AA.
AC   Q18B66;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE   AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN   Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=CD630_12030;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
CC   -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC       insoluble oligonucleotides, which are then degraded further into small
CC       acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC         direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC   -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00378}.
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DR   EMBL; AM180355; CAJ68057.1; -; Genomic_DNA.
DR   RefSeq; WP_003438130.1; NZ_CP010905.2.
DR   RefSeq; YP_001087696.1; NC_009089.1.
DR   AlphaFoldDB; Q18B66; -.
DR   STRING; 272563.CD630_12030; -.
DR   EnsemblBacteria; CAJ68057; CAJ68057; CD630_12030.
DR   GeneID; 66353614; -.
DR   KEGG; cdf:CD630_12030; -.
DR   KEGG; pdc:CDIF630_01352; -.
DR   PATRIC; fig|272563.120.peg.1254; -.
DR   eggNOG; COG1570; Bacteria.
DR   OMA; WPAVRFE; -.
DR   PhylomeDB; Q18B66; -.
DR   BioCyc; PDIF272563:G12WB-1334-MON; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR   GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd04489; ExoVII_LU_OBF; 1.
DR   HAMAP; MF_00378; Exonuc_7_L; 1.
DR   InterPro; IPR003753; Exonuc_VII_L.
DR   InterPro; IPR020579; Exonuc_VII_lsu_C.
DR   InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR   PANTHER; PTHR30008; PTHR30008; 2.
DR   Pfam; PF02601; Exonuc_VII_L; 2.
DR   Pfam; PF13742; tRNA_anti_2; 1.
DR   TIGRFAMs; TIGR00237; xseA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..401
FT                   /note="Exodeoxyribonuclease 7 large subunit"
FT                   /id="PRO_0000273652"
SQ   SEQUENCE   401 AA;  45299 MW;  20CDA7CD8F741E64 CRC64;
     MKLRALDISE ANSYIKRILI NDPILSNLKV KGEISNFKVH SSGNVYLSLK DETSKLNCVI
     FKSNFNRNLK LDNGVKIIAN GYISVYERDG AYQLYINEIE IEGIGNLHIE FNRLKEKLNK
     EGLFDPKYKI PIPKMPNSIG VITSPTGAVI RDIINVIKRR YPKVNIKLYP VTVQGDKSAE
     EICEAIRFFN HMKNVDTLIV GRGGGSIEEL WSFNDEMVAR EVFNSQIPII SAVGHETDFT
     ICDFVSDMRA PTPSAAAEIA TPSLDDINYK LGNIKSRMSK SLTNQIELDQ YRLETVFNKI
     NNYLDSYTIK DKVIQLDKIY DKIIFGIENN LKLEDEKLVK IGALLHNLSP LATMDRGYSI
     TQKNGKVINS IKGLKIKDSI DIVLKDGNLE CMIDKIENKE G