EX7L_CLOK5
ID EX7L_CLOK5 Reviewed; 400 AA.
AC A5N7I9;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=CKL_1228;
OS Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=431943;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA Hagemeier C., Thauer R.K., Gottschalk G.;
RT "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT metabolic features.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
CC -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC insoluble oligonucleotides, which are then degraded further into small
CC acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00378}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC Rule:MF_00378}.
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DR EMBL; CP000673; EDK33270.1; -; Genomic_DNA.
DR RefSeq; WP_012101611.1; NC_009706.1.
DR AlphaFoldDB; A5N7I9; -.
DR SMR; A5N7I9; -.
DR STRING; 431943.CKL_1228; -.
DR EnsemblBacteria; EDK33270; EDK33270; CKL_1228.
DR KEGG; ckl:CKL_1228; -.
DR eggNOG; COG1570; Bacteria.
DR HOGENOM; CLU_023625_2_0_9; -.
DR OMA; WPAVRFE; -.
DR OrthoDB; 1371775at2; -.
DR Proteomes; UP000002411; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04489; ExoVII_LU_OBF; 1.
DR HAMAP; MF_00378; Exonuc_7_L; 1.
DR InterPro; IPR003753; Exonuc_VII_L.
DR InterPro; IPR020579; Exonuc_VII_lsu_C.
DR InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR PANTHER; PTHR30008; PTHR30008; 2.
DR Pfam; PF02601; Exonuc_VII_L; 1.
DR Pfam; PF13742; tRNA_anti_2; 1.
DR TIGRFAMs; TIGR00237; xseA; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT CHAIN 1..400
FT /note="Exodeoxyribonuclease 7 large subunit"
FT /id="PRO_1000079981"
SQ SEQUENCE 400 AA; 45366 MW; 0EC035B3B4A03829 CRC64;
MYIKTLTVSD INRYIKKTLD NDFILGNCSV KGEVSNFKFH SSGHMYFSLK DKFSKINCIM
FKSSVEKLNF MPGDGMKVIV KGRISLYEKE GVYQLYCSEM KPDGMGELYL AFEKLKIELE
KKGLFDISHK KKIPLYAKKI GVITSPTGAA VKDIINVTRR RNKKIELLIY PSLVQGTGAS
DNIIKGIETF NSMEDVELII IARGGGSIEE LWCFNDEKLA EAVYSSKKPI ITGVGHEIDY
TIVDFVSDMR APTPSAAAEI GVFSLEEYVQ KILNYKNKLY NSVKNTVNDK KNRLAFVKKT
LEVNNPLTYI ANEYENIDKI KESLNFKIKV IINGKKEKLG KINALLSAHN PLNILNKGYC
IIEDEQKNVI SSIEELNKKY KVDIIMKDGT SKVELIHYKK
