ID   EX7L_GEOKA              Reviewed;         448 AA.
AC   Q75TC0; Q5KXA6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE   AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN   Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=GK2395;
GN   ORFNames=GKC02;
OS   Geobacillus kaustophilus (strain HTA426).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=235909;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15168170; DOI=10.1007/s00792-004-0394-3;
RA   Takami H., Nishi S., Lu J., Shimamura S., Takaki Y.;
RT   "Genomic characterization of thermophilic Geobacillus species isolated from
RT   the deepest sea mud of the Mariana Trench.";
RL   Extremophiles 8:351-356(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT   Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
CC   -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC       insoluble oligonucleotides, which are then degraded further into small
CC       acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC         direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC   -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00378}.
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DR   EMBL; AB126620; BAD18358.1; -; Genomic_DNA.
DR   EMBL; BA000043; BAD76680.1; -; Genomic_DNA.
DR   RefSeq; WP_011231877.1; NC_006510.1.
DR   AlphaFoldDB; Q75TC0; -.
DR   SMR; Q75TC0; -.
DR   STRING; 235909.GK2395; -.
DR   EnsemblBacteria; BAD76680; BAD76680; GK2395.
DR   KEGG; gka:GK2395; -.
DR   eggNOG; COG1570; Bacteria.
DR   HOGENOM; CLU_023625_3_1_9; -.
DR   OMA; WPAVRFE; -.
DR   Proteomes; UP000001172; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR   GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd04489; ExoVII_LU_OBF; 1.
DR   HAMAP; MF_00378; Exonuc_7_L; 1.
DR   InterPro; IPR003753; Exonuc_VII_L.
DR   InterPro; IPR020579; Exonuc_VII_lsu_C.
DR   InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR   PANTHER; PTHR30008; PTHR30008; 1.
DR   Pfam; PF02601; Exonuc_VII_L; 1.
DR   Pfam; PF13742; tRNA_anti_2; 1.
DR   TIGRFAMs; TIGR00237; xseA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..448
FT                   /note="Exodeoxyribonuclease 7 large subunit"
FT                   /id="PRO_0000303788"
SQ   SEQUENCE   448 AA;  51182 MW;  1F69A7AFD12987DE CRC64;
     MEVKYVTVGA LTKYIKRKFD VDPHLRDLWV KGEISNFKQH SRGHMYFTLK DSQGRIAAVM
     FAGYNQHLPF RPEDGMNVLA RGEISVYEPN GNYQLYVKEM QPEGVGSLYL AYEQLKQRLE
     AEGLFSPVHK KPLPAFPRCI GVVTSPTGAA VRDIMTTIRR RYPLAKVILF PALVQGDGAA
     PSIVRAIERA NSFGGVDVLI VGRGGGSIEE LWAFNEEIVA RAIFASKIPI ISAVGHETDF
     TIADFVADLR APTPTAAAEM AAPHVGELAE RLAERKWRLI RAMKEQLAAD KEQLRRLQGS
     YAFRYPKRLY EQKEQQLDAL LERLSRQRER LVDKHRQRLH ELDMRLWRHH PAAELERMKE
     RQKAAANALE KAMRTAVERH RFRFRSNLAR LEALSPLRIM ERGYSLVYNE QGELVKRTNQ
     LRIGEHLSIQ VQDGRVDCQV TGVEEKHV