ID   EX7L_LACAC              Reviewed;         455 AA.
AC   Q5FJG6;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE   AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN   Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=LBA1331;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC       insoluble oligonucleotides, which are then degraded further into small
CC       acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC         direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC   -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00378}.
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DR   EMBL; CP000033; AAV43158.1; -; Genomic_DNA.
DR   RefSeq; WP_003547941.1; NC_006814.3.
DR   RefSeq; YP_194189.1; NC_006814.3.
DR   AlphaFoldDB; Q5FJG6; -.
DR   SMR; Q5FJG6; -.
DR   STRING; 272621.LBA1331; -.
DR   EnsemblBacteria; AAV43158; AAV43158; LBA1331.
DR   GeneID; 56942915; -.
DR   KEGG; lac:LBA1331; -.
DR   PATRIC; fig|272621.13.peg.1259; -.
DR   eggNOG; COG1570; Bacteria.
DR   HOGENOM; CLU_023625_3_1_9; -.
DR   OMA; WPAVRFE; -.
DR   BioCyc; LACI272621:G1G49-1308-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR   GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd04489; ExoVII_LU_OBF; 1.
DR   HAMAP; MF_00378; Exonuc_7_L; 1.
DR   InterPro; IPR003753; Exonuc_VII_L.
DR   InterPro; IPR020579; Exonuc_VII_lsu_C.
DR   InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR   PANTHER; PTHR30008; PTHR30008; 1.
DR   Pfam; PF02601; Exonuc_VII_L; 1.
DR   Pfam; PF13742; tRNA_anti_2; 1.
DR   TIGRFAMs; TIGR00237; xseA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..455
FT                   /note="Exodeoxyribonuclease 7 large subunit"
FT                   /id="PRO_0000273662"
SQ   SEQUENCE   455 AA;  52602 MW;  D7634B989436F987 CRC64;
     MAENKYLTVT DLNYYITQKF KNDPYLHKVF LQGELSNFRY RRNSHQYFSL KDEKSKINVV
     MFRSYFDKVK FKPEEGMKVY VTGYVSVYGP QGSYQFYAEN MEPAGLGALY EQLKQLQVKL
     AKEGLFNPEH KKKIPRFPDR IAVVTSASGA VIHDIMVTAN RRFPHAEIDL FPAQVQGDTA
     AESLVRAMRQ IAAEGDKYDV MIIGRGGGSL EDLWPFNEEE VVRQVYNMQM PVISSVGHET
     DTTLCDLVAD ARAATPTAAA EYATPNLMDE LAGIHQLQSR LFSSMQTIIR QKRDRLNRIQ
     NSIIMREPTR LYDQQIETVD RLKQRLQNSM QNKLDHSKQD YRLLNQRLFA VNPDKQINQM
     KQQRLFLAKR LSDNMQHYLK DKRNIFAQIV QQLDDYSPLK TLERGFVYTT DRDGKTISSV
     KQVNKNDSLN LHFKDGQVAA KVVEVKEEKN ANEEK