ID   EX7L_PSEF5              Reviewed;         459 AA.
AC   Q4K6V9;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            EC=3.1.11.6 {ECO:0000255|HAMAP-Rule:MF_00378};
DE   AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
DE            Short=Exonuclease VII large subunit {ECO:0000255|HAMAP-Rule:MF_00378};
GN   Name=xseA {ECO:0000255|HAMAP-Rule:MF_00378}; OrderedLocusNames=PFL_4944;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC       insoluble oligonucleotides, which are then degraded further into small
CC       acid-soluble oligonucleotides. {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC         direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00378};
CC   -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00378}.
CC   -!- SIMILARITY: Belongs to the XseA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00378}.
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DR   EMBL; CP000076; AAY94173.1; -; Genomic_DNA.
DR   RefSeq; WP_011063197.1; NC_004129.6.
DR   AlphaFoldDB; Q4K6V9; -.
DR   SMR; Q4K6V9; -.
DR   STRING; 220664.PFL_4944; -.
DR   EnsemblBacteria; AAY94173; AAY94173; PFL_4944.
DR   GeneID; 57477926; -.
DR   KEGG; pfl:PFL_4944; -.
DR   PATRIC; fig|220664.5.peg.5065; -.
DR   eggNOG; COG1570; Bacteria.
DR   HOGENOM; CLU_023625_3_1_6; -.
DR   OMA; LVWPVKV; -.
DR   OrthoDB; 1371775at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR   GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd04489; ExoVII_LU_OBF; 1.
DR   HAMAP; MF_00378; Exonuc_7_L; 1.
DR   InterPro; IPR003753; Exonuc_VII_L.
DR   InterPro; IPR020579; Exonuc_VII_lsu_C.
DR   InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR   PANTHER; PTHR30008; PTHR30008; 1.
DR   Pfam; PF02601; Exonuc_VII_L; 1.
DR   Pfam; PF13742; tRNA_anti_2; 1.
DR   TIGRFAMs; TIGR00237; xseA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..459
FT                   /note="Exodeoxyribonuclease 7 large subunit"
FT                   /id="PRO_0000273676"
SQ   SEQUENCE   459 AA;  51063 MW;  BC24E357D45B15A3 CRC64;
     MIKDPFARLG LDREVLTVTQ LNGRARVLLE DVFSNIWVEG EISNLARPAS GHVYFTLKDS
     GAQVRCALFR QNAARVRQAL KDGLAVKVRG KVSLFEGRGD YQLILDTVEP AGDGALRLAF
     DALKDKLSAE GLFSAERKVP LPAHPQRIGI ISSPTGAVIR DIISVFRRRA PQVVLTLIPT
     AVQGREATAQ IVRALQLADA RGFDALILAR GGGSLEDLWC FNEEAVARAV DACVTPIVSA
     VGHETDVSIS DFVADVRAPT PSAAAELLAP DSSDLQRRVD NLHRRLVMRM RDRLVRDRLR
     LDGLARRLRH PGERLRQQAQ RLDDLDMRLR RAFERSLNTR RERLIRLETR LGAQHPGRQL
     ALLRQRLDSL AERLPRAMRE GLKGRRLQLH SQMQTLHVVS PLATLGRGYS ILLDDRGQAI
     RSAGQTRPGQ RLSARLGEGE LQVRVEDNHL TPVTLSLLD