AGUA_ENTFA
ID AGUA_ENTFA Reviewed; 365 AA.
AC Q837U5;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Putative agmatine deiminase {ECO:0000255|HAMAP-Rule:MF_01841};
DE EC=3.5.3.12 {ECO:0000255|HAMAP-Rule:MF_01841, ECO:0000269|PubMed:17028272};
DE AltName: Full=Agmatine iminohydrolase {ECO:0000255|HAMAP-Rule:MF_01841};
GN Name=aguA {ECO:0000255|HAMAP-Rule:MF_01841}; OrderedLocusNames=EF_0734;
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583;
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS), CATALYTIC ACTIVITY, ACTIVE SITE,
RP AND SUBUNIT.
RX PubMed=17028272; DOI=10.1128/jb.01216-06;
RA Llacer J.L., Polo L.M., Tavarez S., Alarcon B., Hilario R., Rubio V.;
RT "The gene cluster for agmatine catabolism of Enterococcus faecalis: study
RT of recombinant putrescine transcarbamylase and agmatine deiminase and a
RT snapshot of agmatine deiminase catalyzing its reaction.";
RL J. Bacteriol. 189:1254-1265(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=agmatine + H2O = N-carbamoylputrescine + NH4(+);
CC Xref=Rhea:RHEA:18037, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:58145, ChEBI:CHEBI:58318; EC=3.5.3.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01841,
CC ECO:0000269|PubMed:17028272};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18038;
CC Evidence={ECO:0000269|PubMed:17028272};
CC -!- SUBUNIT: Tetramer of two homodimers. {ECO:0000269|PubMed:17028272}.
CC -!- SIMILARITY: Belongs to the agmatine deiminase family.
CC {ECO:0000255|HAMAP-Rule:MF_01841}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO80553.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAO80553.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE016830; AAO80553.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_814483.2; NC_004668.1.
DR RefSeq; WP_002363185.1; NZ_KE136527.1.
DR PDB; 2JER; X-ray; 1.65 A; A/B/C/D/E/F/G/H=1-365.
DR PDBsum; 2JER; -.
DR AlphaFoldDB; Q837U5; -.
DR SMR; Q837U5; -.
DR STRING; 226185.EF_0734; -.
DR EnsemblBacteria; AAO80553; AAO80553; EF_0734.
DR KEGG; efa:EF0734; -.
DR PATRIC; fig|226185.45.peg.2675; -.
DR eggNOG; COG2957; Bacteria.
DR HOGENOM; CLU_037682_0_0_9; -.
DR OMA; GNVACIT; -.
DR BRENDA; 3.5.3.12; 2095.
DR SABIO-RK; Q837U5; -.
DR EvolutionaryTrace; Q837U5; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0047632; F:agmatine deiminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004668; F:protein-arginine deiminase activity; IEA:InterPro.
DR GO; GO:0009446; P:putrescine biosynthetic process; IEA:InterPro.
DR HAMAP; MF_01841; Agmatine_deimin; 1.
DR InterPro; IPR017754; Agmatine_deiminase.
DR InterPro; IPR007466; Peptidyl-Arg-deiminase_porph.
DR PANTHER; PTHR31377; PTHR31377; 1.
DR Pfam; PF04371; PAD_porph; 1.
DR TIGRFAMs; TIGR03380; agmatine_aguA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..365
FT /note="Putative agmatine deiminase"
FT /id="PRO_0000194324"
FT ACT_SITE 357
FT /note="Amidino-cysteine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01841,
FT ECO:0000269|PubMed:17028272, ECO:0007744|PDB:2JER"
FT BINDING 214
FT /ligand="agmatine"
FT /ligand_id="ChEBI:CHEBI:58145"
FT /evidence="ECO:0000250|UniProtKB:G7JT50"
FT BINDING 220
FT /ligand="agmatine"
FT /ligand_id="ChEBI:CHEBI:58145"
FT /evidence="ECO:0000250|UniProtKB:G7JT50"
FT HELIX 10..13
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 24..30
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 39..42
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 43..57
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 71..77
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 94..97
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 109..115
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 121..124
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 126..128
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 136..144
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 148..155
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 158..160
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 165..172
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 173..176
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 187..198
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 201..206
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 212..215
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 219..221
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 223..227
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 230..234
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 244..255
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 266..270
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 308..310
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 313..318
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 324..334
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 338..344
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 346..349
FT /evidence="ECO:0007829|PDB:2JER"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:2JER"
FT HELIX 356..358
FT /evidence="ECO:0007829|PDB:2JER"
FT STRAND 360..363
FT /evidence="ECO:0007829|PDB:2JER"
SQ SEQUENCE 365 AA; 41199 MW; 9B8ABD927D92AD58 CRC64;
MAKRIVGSTP KQDGFRMPGE FEPQEKVWMI WPERPDNWRD GGKPVQEAFT NVAKAISQFT
PMNVVVSQQQ FQNCRRQLPP EITVYEMSNN DAWVRDCGPS FVINDHGEIR GVDWTFNAWG
GLVDGLYFPW DQDDLVAQKI CEIEHVDSYR TDDFVLEGGS FHVDGQGTVL TTEMCLLSEG
RNPQLSKEAI EQKLCDYLNV EKVLWLGDGI DPEETNGHVD DVACFIAPGE VACIYTEDQN
SPFYEAAQDA YQRLLKMTDA KGRQLKVHKL CCPVKNVTIK GSFKIDFVEG TMPREDGDIC
IASYMNFLIT NDGVIVPQYG DENDHLALEQ VQTMFPDKKI VGVNTVEVVY GGGNIHCITQ
QEPKR
