EXBB_ECOLI
ID EXBB_ECOLI Reviewed; 244 AA.
AC P0ABU7; P18783; Q2M9J2;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Biopolymer transport protein ExbB;
GN Name=exbB; OrderedLocusNames=b3006, JW2974;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2670903; DOI=10.1128/jb.171.9.5117-5126.1989;
RA Eick-Helmerich K., Braun V.;
RT "Import of biopolymers into Escherichia coli: nucleotide sequences of the
RT exbB and exbD genes are homologous to those of the tolQ and tolR genes,
RT respectively.";
RL J. Bacteriol. 171:5117-5126(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY.
RX PubMed=8449962; DOI=10.1016/s0021-9258(18)53424-4;
RA Kampfenkel K., Braun V.;
RT "Topology of the ExbB protein in the cytoplasmic membrane of Escherichia
RT coli.";
RL J. Biol. Chem. 268:6050-6057(1993).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Involved in the TonB-dependent energy-dependent transport of
CC various receptor-bound substrates. Protects ExbD from proteolytic
CC degradation and functionally stabilizes TonB.
CC -!- SUBUNIT: The accessory proteins ExbB and ExbD seem to form a complex
CC with TonB.
CC -!- INTERACTION:
CC P0ABU7; P0ABV2: exbD; NbExp=7; IntAct=EBI-6399986, EBI-6417016;
CC P0ABU7; P02929: tonB; NbExp=2; IntAct=EBI-6399986, EBI-6399993;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Induced 2.4-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the ExbB/TolQ family. {ECO:0000305}.
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DR EMBL; M28819; AAA23732.1; -; Genomic_DNA.
DR EMBL; U28377; AAA69173.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76042.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77064.1; -; Genomic_DNA.
DR PIR; D65087; BVECXB.
DR RefSeq; NP_417479.1; NC_000913.3.
DR RefSeq; WP_000527844.1; NZ_STEB01000001.1.
DR PDB; 5SV0; X-ray; 2.60 A; A/B/C/D/E/F/G/H/I/J=1-244.
DR PDB; 5SV1; X-ray; 3.50 A; A/B/C/D/E/F/G/H/I/J=1-244.
DR PDB; 5ZFP; X-ray; 2.84 A; A/B/C/D/E/F/G/H/I/J/K/L=1-244.
DR PDB; 5ZFU; EM; 6.70 A; A/B/C/D/E/F=1-244.
DR PDB; 5ZFV; EM; 7.10 A; A/B/C/D/E=1-244.
DR PDB; 6TYI; EM; 3.30 A; A/B/C/D/E=1-244.
DR PDBsum; 5SV0; -.
DR PDBsum; 5SV1; -.
DR PDBsum; 5ZFP; -.
DR PDBsum; 5ZFU; -.
DR PDBsum; 5ZFV; -.
DR PDBsum; 6TYI; -.
DR AlphaFoldDB; P0ABU7; -.
DR SMR; P0ABU7; -.
DR BioGRID; 4261412; 998.
DR BioGRID; 849794; 2.
DR ComplexPortal; CPX-1083; Cobalamin outer membrane transporter complex.
DR ComplexPortal; CPX-2843; Ferrichrome outer membrane transporter complex.
DR ComplexPortal; CPX-3576; Ferric-citrate outer membrane transporter complex.
DR ComplexPortal; CPX-3577; Ferric-catecholate outer membrane transporter complex.
DR ComplexPortal; CPX-3578; Ferric-enterobactin outer membrane transporter complex.
DR ComplexPortal; CPX-3579; Ferric-coprogen outer membrane transporter complex.
DR ComplexPortal; CPX-3580; fiu outer membrane transporter complex.
DR ComplexPortal; CPX-3585; Uncharacterized yncD-DHBS outer membrane transporter complex.
DR DIP; DIP-47841N; -.
DR IntAct; P0ABU7; 29.
DR STRING; 511145.b3006; -.
DR TCDB; 1.A.30.2.1; the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.
DR jPOST; P0ABU7; -.
DR PaxDb; P0ABU7; -.
DR PRIDE; P0ABU7; -.
DR EnsemblBacteria; AAC76042; AAC76042; b3006.
DR EnsemblBacteria; BAE77064; BAE77064; BAE77064.
DR GeneID; 67415048; -.
DR GeneID; 945420; -.
DR KEGG; ecj:JW2974; -.
DR KEGG; eco:b3006; -.
DR PATRIC; fig|1411691.4.peg.3723; -.
DR EchoBASE; EB0267; -.
DR eggNOG; COG0811; Bacteria.
DR HOGENOM; CLU_053325_0_2_6; -.
DR InParanoid; P0ABU7; -.
DR OMA; PHMVKVG; -.
DR PhylomeDB; P0ABU7; -.
DR BioCyc; EcoCyc:EG10271-MON; -.
DR BioCyc; MetaCyc:EG10271-MON; -.
DR PRO; PR:P0ABU7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0098797; C:plasma membrane protein complex; IDA:EcoCyc.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0031992; F:energy transducer activity; IMP:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR GO; GO:0015889; P:cobalamin transport; IC:ComplexPortal.
DR GO; GO:0055072; P:iron ion homeostasis; IC:ComplexPortal.
DR GO; GO:0055065; P:metal ion homeostasis; IC:ComplexPortal.
DR GO; GO:0017038; P:protein import; IBA:GO_Central.
DR GO; GO:0050821; P:protein stabilization; IMP:EcoCyc.
DR InterPro; IPR002898; MotA_ExbB_proton_chnl.
DR InterPro; IPR014164; TonB_ExbB_1.
DR Pfam; PF01618; MotA_ExbB; 1.
DR TIGRFAMs; TIGR02797; exbB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriocin transport; Cell inner membrane; Cell membrane;
KW Membrane; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..244
FT /note="Biopolymer transport protein ExbB"
FT /id="PRO_0000145799"
FT TOPO_DOM 1..15
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 16..39
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 40..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 128..155
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 156..161
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 162..194
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 195..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="Q -> L (in Ref. 1; AAA23732)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="R -> A (in Ref. 1; AAA23732)"
FT /evidence="ECO:0000305"
FT HELIX 12..16
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 21..62
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 68..75
FT /evidence="ECO:0007829|PDB:5SV0"
FT TURN 79..81
FT /evidence="ECO:0007829|PDB:5SV1"
FT HELIX 83..97
FT /evidence="ECO:0007829|PDB:5SV0"
FT TURN 98..100
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 104..107
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 110..126
FT /evidence="ECO:0007829|PDB:5SV0"
FT TURN 127..129
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 130..159
FT /evidence="ECO:0007829|PDB:5SV0"
FT TURN 160..163
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 167..169
FT /evidence="ECO:0007829|PDB:5SV0"
FT HELIX 171..232
FT /evidence="ECO:0007829|PDB:5SV0"
SQ SEQUENCE 244 AA; 26287 MW; CEB63893A80BA71F CRC64;
MGNNLMQTDL SVWGMYQHAD IVVKCVMIGL ILASVVTWAI FFSKSVEFFN QKRRLKREQQ
LLAEARSLNQ ANDIAADFGS KSLSLHLLNE AQNELELSEG SDDNEGIKER TSFRLERRVA
AVGRQMGRGN GYLATIGAIS PFVGLFGTVW GIMNSFIGIA QTQTTNLAVV APGIAEALLA
TAIGLVAAIP AVVIYNVFAR QIGGFKAMLG DVAAQVLLLQ SRDLDLEASA AAHPVRVAQK
LRAG
