EXBD_ECOLI
ID EXBD_ECOLI Reviewed; 141 AA.
AC P0ABV2; P18784; Q2M9J3;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Biopolymer transport protein ExbD;
GN Name=exbD; OrderedLocusNames=b3005, JW2973;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2670903; DOI=10.1128/jb.171.9.5117-5126.1989;
RA Eick-Helmerich K., Braun V.;
RT "Import of biopolymers into Escherichia coli: nucleotide sequences of the
RT exbB and exbD genes are homologous to those of the tolQ and tolR genes,
RT respectively.";
RL J. Bacteriol. 171:5117-5126(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY.
RX PubMed=1644779; DOI=10.1128/jb.174.16.5485-5487.1992;
RA Kampfenkel K., Braun V.;
RT "Membrane topology of the Escherichia coli ExbD protein.";
RL J. Bacteriol. 174:5485-5487(1992).
RN [5]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: Involved in the TonB-dependent energy-dependent transport of
CC various receptor-bound substrates.
CC -!- SUBUNIT: The accessory proteins ExbB and ExbD seem to form a complex
CC with TonB.
CC -!- INTERACTION:
CC P0ABV2; P0ABU7: exbB; NbExp=7; IntAct=EBI-6417016, EBI-6399986;
CC P0ABV2; P0ABV2: exbD; NbExp=3; IntAct=EBI-6417016, EBI-6417016;
CC P0ABV2; P02929: tonB; NbExp=3; IntAct=EBI-6417016, EBI-6399993;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Single-pass type II membrane
CC protein.
CC -!- INDUCTION: Induced 2.1-fold by hydroxyurea.
CC {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the ExbD/TolR family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M28819; AAA23733.1; -; Genomic_DNA.
DR EMBL; U28377; AAA69172.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76041.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77063.1; -; Genomic_DNA.
DR PIR; JV0030; BVECED.
DR RefSeq; NP_417478.1; NC_000913.3.
DR RefSeq; WP_001240712.1; NZ_STEB01000001.1.
DR PDB; 2PFU; NMR; -; A=44-141.
DR PDB; 5SV1; X-ray; 3.50 A; Y/Z=1-49.
DR PDB; 5ZFU; EM; 6.70 A; G/H/I=19-40.
DR PDB; 5ZFV; EM; 7.10 A; F=19-40.
DR PDB; 6TYI; EM; 3.30 A; Y/Z=1-141.
DR PDBsum; 2PFU; -.
DR PDBsum; 5SV1; -.
DR PDBsum; 5ZFU; -.
DR PDBsum; 5ZFV; -.
DR PDBsum; 6TYI; -.
DR AlphaFoldDB; P0ABV2; -.
DR BMRB; P0ABV2; -.
DR SMR; P0ABV2; -.
DR BioGRID; 4261413; 269.
DR BioGRID; 850702; 2.
DR ComplexPortal; CPX-1083; Cobalamin outer membrane transporter complex.
DR ComplexPortal; CPX-2843; Ferrichrome outer membrane transporter complex.
DR ComplexPortal; CPX-3576; Ferric-citrate outer membrane transporter complex.
DR ComplexPortal; CPX-3577; Ferric-catecholate outer membrane transporter complex.
DR ComplexPortal; CPX-3578; Ferric-enterobactin outer membrane transporter complex.
DR ComplexPortal; CPX-3579; Ferric-coprogen outer membrane transporter complex.
DR ComplexPortal; CPX-3580; fiu outer membrane transporter complex.
DR ComplexPortal; CPX-3585; Uncharacterized yncD-DHBS outer membrane transporter complex.
DR DIP; DIP-47973N; -.
DR IntAct; P0ABV2; 4.
DR STRING; 511145.b3005; -.
DR TCDB; 1.A.30.2.1; the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.
DR jPOST; P0ABV2; -.
DR PaxDb; P0ABV2; -.
DR PRIDE; P0ABV2; -.
DR EnsemblBacteria; AAC76041; AAC76041; b3005.
DR EnsemblBacteria; BAE77063; BAE77063; BAE77063.
DR GeneID; 67415049; -.
DR GeneID; 946345; -.
DR KEGG; ecj:JW2973; -.
DR KEGG; eco:b3005; -.
DR PATRIC; fig|1411691.4.peg.3724; -.
DR EchoBASE; EB0268; -.
DR eggNOG; COG0848; Bacteria.
DR HOGENOM; CLU_085305_1_3_6; -.
DR InParanoid; P0ABV2; -.
DR OMA; TQGKKDT; -.
DR PhylomeDB; P0ABV2; -.
DR BioCyc; EcoCyc:EG10272-MON; -.
DR BioCyc; MetaCyc:EG10272-MON; -.
DR EvolutionaryTrace; P0ABV2; -.
DR PRO; PR:P0ABV2; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0098797; C:plasma membrane protein complex; IDA:EcoCyc.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0031992; F:energy transducer activity; IMP:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR GO; GO:0015889; P:cobalamin transport; IC:ComplexPortal.
DR GO; GO:0055072; P:iron ion homeostasis; IC:ComplexPortal.
DR GO; GO:0055065; P:metal ion homeostasis; IC:ComplexPortal.
DR GO; GO:0050821; P:protein stabilization; IMP:EcoCyc.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR003400; ExbD.
DR InterPro; IPR014170; TonB_ExbD_1.
DR PANTHER; PTHR30558; PTHR30558; 1.
DR Pfam; PF02472; ExbD; 1.
DR TIGRFAMs; TIGR02803; ExbD_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriocin transport; Cell inner membrane; Cell membrane;
KW Membrane; Protein transport; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..141
FT /note="Biopolymer transport protein ExbD"
FT /id="PRO_0000129117"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:1644779"
FT TRANSMEM 23..43
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000305"
FT TOPO_DOM 44..141
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:1644779"
FT HELIX 21..36
FT /evidence="ECO:0007829|PDB:6TYI"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:6TYI"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:2PFU"
FT TURN 70..72
FT /evidence="ECO:0007829|PDB:2PFU"
FT STRAND 73..76
FT /evidence="ECO:0007829|PDB:2PFU"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:2PFU"
FT HELIX 86..93
FT /evidence="ECO:0007829|PDB:2PFU"
FT STRAND 94..96
FT /evidence="ECO:0007829|PDB:2PFU"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:2PFU"
FT HELIX 112..124
FT /evidence="ECO:0007829|PDB:2PFU"
SQ SEQUENCE 141 AA; 15527 MW; 25A539A2FAEB9F6C CRC64;
MAMHLNENLD DNGEMHDINV TPFIDVMLVL LIIFMVAAPL ATVDVKVNLP ASTSTPQPRP
EKPVYLSVKA DNSMFIGNDP VTDETMITAL NALTEGKKDT TIFFRADKTV DYETLMKVMD
TLHQAGYLKI GLVGEETAKA K
