AGUB_ORYSJ
ID AGUB_ORYSJ Reviewed; 301 AA.
AC Q93XI4; B7E6S1;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=N-carbamoylputrescine amidase;
DE EC=3.5.1.53;
GN Name=CPA; OrderedLocusNames=Os02g0533900, LOC_Os02g33080;
GN ORFNames=B1136H02.7, P0605D08.40;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Leaf;
RA Kimura K., Nakada Y., Itoh Y.;
RT "Rice cDNA encoding a putative carbon-nitrogen hydrolase of the beta-alnine
RT synthase/nitrilase family.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Involved in polyamine biosynthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + H2O + N-carbamoylputrescine = CO2 + NH4(+) +
CC putrescine; Xref=Rhea:RHEA:22284, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:58318, ChEBI:CHEBI:326268; EC=3.5.1.53;
CC -!- PATHWAY: Amine and polyamine biosynthesis; putrescine biosynthesis via
CC agmatine pathway; putrescine from N-carbamoylputrescine (amidase
CC route): step 1/1.
CC -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC {ECO:0000305}.
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DR EMBL; AB062681; BAB59126.1; -; mRNA.
DR EMBL; AP005110; BAD28433.1; -; Genomic_DNA.
DR EMBL; AP005798; BAD29288.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08947.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79051.1; -; Genomic_DNA.
DR EMBL; AK061722; BAG88068.1; -; mRNA.
DR EMBL; AK106147; BAG97599.1; -; mRNA.
DR RefSeq; XP_015625266.1; XM_015769780.1.
DR AlphaFoldDB; Q93XI4; -.
DR SMR; Q93XI4; -.
DR STRING; 4530.OS02T0533900-01; -.
DR PaxDb; Q93XI4; -.
DR PRIDE; Q93XI4; -.
DR EnsemblPlants; Os02t0533900-01; Os02t0533900-01; Os02g0533900.
DR EnsemblPlants; Os02t0533900-02; Os02t0533900-02; Os02g0533900.
DR GeneID; 4329560; -.
DR Gramene; Os02t0533900-01; Os02t0533900-01; Os02g0533900.
DR Gramene; Os02t0533900-02; Os02t0533900-02; Os02g0533900.
DR KEGG; osa:4329560; -.
DR eggNOG; KOG0806; Eukaryota.
DR HOGENOM; CLU_030130_4_0_1; -.
DR InParanoid; Q93XI4; -.
DR OMA; NAPYFCQ; -.
DR OrthoDB; 996578at2759; -.
DR PlantReactome; R-OSA-1119304; Putrescine biosynthesis II.
DR UniPathway; UPA00534; UER00286.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q93XI4; OS.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IBA:GO_Central.
DR GO; GO:0050126; F:N-carbamoylputrescine amidase activity; IBA:GO_Central.
DR GO; GO:0033388; P:putrescine biosynthetic process from arginine; IBA:GO_Central.
DR Gene3D; 3.60.110.10; -; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR017755; N-carbamoylputrescine_amidase.
DR Pfam; PF00795; CN_hydrolase; 1.
DR SUPFAM; SSF56317; SSF56317; 1.
DR TIGRFAMs; TIGR03381; agmatine_aguB; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Polyamine biosynthesis; Reference proteome.
FT CHAIN 1..301
FT /note="N-carbamoylputrescine amidase"
FT /id="PRO_0000261602"
FT DOMAIN 11..269
FT /note="CN hydrolase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 50
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 123
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 160
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ SEQUENCE 301 AA; 33463 MW; 86B62C561CE6BFE1 CRC64;
MAGGGGAGSK VSVAAVQFAC TDVESENVDT AERLIREAHK KGANIVLVQE LFEGQYFCQA
QRLDFFQRAK PYKGNPTIIR FQKLAKELEV VIPVSFFEEA NNAHYNSVAI IDADGTDLGL
YRKSHIPDGP GYQEKFYFNP GDTGFKAFKT KYATIGVGIC WDQWFPECAR AMVLQGAEIL
FYPTAIGSEP QDNNLDSREH WKRVMQGHAG ANLVPLVASN RIGRETVETE HGESTITFFG
NSFIAGPTGE IVKLANDKDE DVLVAEFDLD EIKSTRHGWG IFRDRRPDLY KVLLTLDGEK
S
