EXD1_DROME
ID EXD1_DROME Reviewed; 265 AA.
AC Q9VU31;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein Exd1 homolog {ECO:0000305};
DE Short=DmExd1 {ECO:0000303|PubMed:26669262};
DE AltName: Full=Exonuclease 3'-5' domain-containing protein 1 homolog {ECO:0000250|UniProtKB:Q8NHP7};
DE AltName: Full=Exonuclease 3'-5' domain-like-containing protein 1 homolog {ECO:0000250|UniProtKB:Q8NHP7};
GN ORFNames=CG11263 {ECO:0000312|FlyBase:FBgn0036330};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP DISRUPTION PHENOTYPE, AND DOMAIN.
RX PubMed=26669262; DOI=10.1016/j.molcel.2015.11.009;
RA Yang Z., Chen K.M., Pandey R.R., Homolka D., Reuter M., Janeiro B.K.,
RA Sachidanandam R., Fauvarque M.O., McCarthy A.A., Pillai R.S.;
RT "PIWI slicing and EXD1 drive biogenesis of nuclear piRNAs from cytosolic
RT targets of the mouse piRNA pathway.";
RL Mol. Cell 61:138-152(2016).
CC -!- FUNCTION: RNA-binding protein. Inactive exonuclease.
CC {ECO:0000250|UniProtKB:H9IUR0}.
CC -!- SUBUNIT: Homodimer (By similarity). {ECO:0000250|UniProtKB:H9IUR0}.
CC -!- DOMAIN: The 3'-5' exonuclease domain lacks the conserved Asp-Glu-Asp-
CC Asp (DEDD) residues that coordinates divalent ions essential for
CC exonuclease activity. {ECO:0000305|PubMed:26669262}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Flies display normal
CC fertility and do not show any transposon activation in the germline.
CC {ECO:0000269|PubMed:26669262}.
CC -!- SIMILARITY: Belongs to the EXD1 family. {ECO:0000305}.
CC -!- CAUTION: It is unclear whether the protein is involved in the piRNA
CC metabolic process. The absence of phenotypes in flies suggests that
CC this function is not conserved. {ECO:0000305|PubMed:26669262}.
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DR EMBL; AE003539; AAF49860.1; -; Genomic_DNA.
DR EMBL; AE014296; ACZ94706.1; -; Genomic_DNA.
DR EMBL; AY118948; AAM50808.1; -; mRNA.
DR RefSeq; NP_001163435.1; NM_001169964.2.
DR RefSeq; NP_648618.1; NM_140361.2.
DR AlphaFoldDB; Q9VU31; -.
DR SMR; Q9VU31; -.
DR STRING; 7227.FBpp0075607; -.
DR PaxDb; Q9VU31; -.
DR PRIDE; Q9VU31; -.
DR DNASU; 39472; -.
DR EnsemblMetazoa; FBtr0075873; FBpp0075607; FBgn0036330.
DR EnsemblMetazoa; FBtr0300699; FBpp0289923; FBgn0036330.
DR GeneID; 39472; -.
DR KEGG; dme:Dmel_CG11263; -.
DR UCSC; CG11263-RA; d. melanogaster.
DR FlyBase; FBgn0036330; CG11263.
DR VEuPathDB; VectorBase:FBgn0036330; -.
DR eggNOG; KOG2405; Eukaryota.
DR HOGENOM; CLU_987886_0_0_1; -.
DR InParanoid; Q9VU31; -.
DR OMA; HYSHRIC; -.
DR OrthoDB; 1126625at2759; -.
DR PhylomeDB; Q9VU31; -.
DR BioGRID-ORCS; 39472; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39472; -.
DR PRO; PR:Q9VU31; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036330; Expressed in cleaving embryo and 10 other tissues.
DR GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR GO; GO:1990923; C:PET complex; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0031047; P:gene silencing by RNA; IBA:GO_Central.
DR GO; GO:0034587; P:piRNA metabolic process; IBA:GO_Central.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; RNA-binding.
FT CHAIN 1..265
FT /note="Protein Exd1 homolog"
FT /id="PRO_0000435801"
FT DOMAIN 32..82
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000255"
SQ SEQUENCE 265 AA; 30279 MW; 9B796B19A612A066 CRC64;
MNKSDGNESF ALDSLPENIR YPFGKRELEI LEKQLDRIVL IYQVDTTYHS ALKDIKDQKI
ISLLVEPSFY GRHHPTSILV VATCNGTYIF DIKALGLIFL ELAKILEADQ PRKVIHYSHR
IADHLLHRQR ISLGGIFDTF VAVCLSSNTR IPYTLPEAIS LVFGLPMEKV TGGCESQRNF
TARPLTHSQM RYLAKLVQLQ HIMHDHLNYS HIFCAEVYRI SLEFSHSYYG LRSCDVAINM
APASSFGFQL LDSFCKKADK ELEQI
