EXEC1_ARATH
ID EXEC1_ARATH Reviewed; 684 AA.
AC Q93YW0; O81880;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Protein EXECUTER 1, chloroplastic {ECO:0000305};
DE Short=AtEX1 {ECO:0000303|PubMed:17540731};
DE Flags: Precursor;
GN Name=EX1 {ECO:0000303|PubMed:17540731};
GN OrderedLocusNames=At4g33630 {ECO:0000312|Araport:AT4G33630};
GN ORFNames=T16L1.120 {ECO:0000312|EMBL:CAA20576.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF GLU-244; PHE-528 AND GLY-646.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=15539603; DOI=10.1126/science.1103178;
RA Wagner D., Przybyla D., Op den Camp R., Kim C., Landgraf F., Lee K.P.,
RA Wuersch M., Laloi C., Nater M., Hideg E., Apel K.;
RT "The genetic basis of singlet oxygen-induced stress responses of
RT Arabidopsis thaliana.";
RL Science 306:1183-1185(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17540731; DOI=10.1073/pnas.0702061104;
RA Lee K.P., Kim C., Landgraf F., Apel K.;
RT "EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from
RT the plastid to the nucleus of Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:10270-10275(2007).
RN [6]
RP FUNCTION.
RX PubMed=22797473; DOI=10.1105/tpc.112.100479;
RA Kim C., Meskauskiene R., Zhang S., Lee K.P., Lakshmanan Ashok M.,
RA Blajecka K., Herrfurth C., Feussner I., Apel K.;
RT "Chloroplasts of Arabidopsis are the source and a primary target of a
RT plant-specific programmed cell death signaling pathway.";
RL Plant Cell 24:3026-3039(2012).
RN [7]
RP INDUCTION.
RX PubMed=23940555; DOI=10.1371/journal.pone.0070289;
RA Sewelam N., Kazan K., Thomas-Hall S.R., Kidd B.N., Manners J.M.,
RA Schenk P.M.;
RT "Ethylene response factor 6 is a regulator of reactive oxygen species
RT signaling in Arabidopsis.";
RL PLoS ONE 8:E70289-E70289(2013).
CC -!- FUNCTION: Together with EX2, enables higher plants to perceive singlet
CC oxygen as a stress signal in plastid that activates a genetically
CC determined nuclear stress response program which triggers a programmed
CC cell death (PCD). This transfer of singlet oxygen-induced stress-
CC related signals from the plastid to the nucleus that triggers
CC genetically controlled PCD pathway is unique to photosynthetic
CC eukaryotes and operates under mild stress conditions, impeding
CC photosystem II (PSII) without causing photooxidative damage of the
CC plant. {ECO:0000269|PubMed:15539603, ECO:0000269|PubMed:17540731,
CC ECO:0000269|PubMed:22797473}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:15539603, ECO:0000269|PubMed:17540731}.
CC -!- INDUCTION: Down-regulated by H(2)O(2). {ECO:0000269|PubMed:23940555}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA20576.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80080.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY059742; AAL24154.1; -; mRNA.
DR EMBL; AL031394; CAA20576.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161583; CAB80080.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86257.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86258.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66693.1; -; Genomic_DNA.
DR EMBL; AY117173; AAM51248.1; -; mRNA.
DR PIR; T04980; T04980.
DR RefSeq; NP_001328575.1; NM_001342225.1.
DR RefSeq; NP_567929.1; NM_119519.4.
DR RefSeq; NP_849488.1; NM_179157.3.
DR AlphaFoldDB; Q93YW0; -.
DR SMR; Q93YW0; -.
DR BioGRID; 14786; 3.
DR STRING; 3702.AT4G33630.2; -.
DR PaxDb; Q93YW0; -.
DR PRIDE; Q93YW0; -.
DR ProteomicsDB; 222331; -.
DR EnsemblPlants; AT4G33630.1; AT4G33630.1; AT4G33630.
DR EnsemblPlants; AT4G33630.2; AT4G33630.2; AT4G33630.
DR EnsemblPlants; AT4G33630.3; AT4G33630.3; AT4G33630.
DR GeneID; 829504; -.
DR Gramene; AT4G33630.1; AT4G33630.1; AT4G33630.
DR Gramene; AT4G33630.2; AT4G33630.2; AT4G33630.
DR Gramene; AT4G33630.3; AT4G33630.3; AT4G33630.
DR KEGG; ath:AT4G33630; -.
DR Araport; AT4G33630; -.
DR TAIR; locus:2134168; AT4G33630.
DR eggNOG; ENOG502QQS3; Eukaryota.
DR HOGENOM; CLU_019201_0_0_1; -.
DR InParanoid; Q93YW0; -.
DR OMA; QWREDDS; -.
DR OrthoDB; 442207at2759; -.
DR PhylomeDB; Q93YW0; -.
DR PRO; PR:Q93YW0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93YW0; baseline and differential.
DR Genevisible; Q93YW0; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0042651; C:thylakoid membrane; IDA:TAIR.
DR GO; GO:0000304; P:response to singlet oxygen; IBA:GO_Central.
DR GO; GO:0010343; P:singlet oxygen-mediated programmed cell death; IGI:TAIR.
DR InterPro; IPR044680; EX1/2.
DR InterPro; IPR001943; UVR_dom.
DR PANTHER; PTHR33917; PTHR33917; 1.
DR Pfam; PF02151; UVR; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Plastid; Reference proteome; Stress response; Transit peptide.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 47..684
FT /note="Protein EXECUTER 1, chloroplastic"
FT /id="PRO_0000021215"
FT DOMAIN 127..162
FT /note="UVR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00217"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..318
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 244
FT /note="E->K: Loss of activity."
FT /evidence="ECO:0000269|PubMed:15539603"
FT MUTAGEN 528
FT /note="F->C: Loss of activity."
FT /evidence="ECO:0000269|PubMed:15539603"
FT MUTAGEN 646
FT /note="G->D: Loss of activity."
FT /evidence="ECO:0000269|PubMed:15539603"
SQ SEQUENCE 684 AA; 76535 MW; F79E7308E0929D9C CRC64;
MPSLSTPPSQ NLAFSPAASA TSSRLTPSSK RSFYPHRLPD PTALCRCSSS SGSNSSSSSS
SDDNPRWDSA IQDVLKSAIK RFDSVLSWYA TLDNDDGEQG SENVEKIDDD WDWDRWKKHF
DQVDDQDRLL SVLKSQLNRA IKREDYEDAA RLKVAIAATA TNDAVGKVMS TFYRALLEER
YKDAVYLRDK AGAGLVGWWS GISEDVKDPF GLIVQITAEH GRYVARSYNP RQLSTSAAGA
PLFEIFLTLD GKGNYKKQAV YLKWKEIFPD VPTMPSRTLT PGRFLTSPGR KEDTGNLAVE
SSEDEESDNS DDDSDLLEES SGFQSFLRDM IPGVKVKVMK VTAPGRVDKD FISKVIEQIA
DEEDEENDLD IEDIDVEDDT KAEIDEKNAD IELESVTDEI IDNNGGREIA VKFVIGDIVD
RLSGNQPLKE SLRSPANLES VENSSFYLRL EKDLNVKESK GVEGTTLVDG KGSRQSRRRI
ENIMGDLAKS IEKEKKISVK MLKDVGELLS LTLSQAQNRQ QLSGLTKFRR IDVTPSLDPL
DGLYIGAHGL YTSEVIHLKR KFGQWKGGKE SKKPTDIEFY EYVEAVKLTG DPYVPAGKVA
FRAKIGRRYE LPHKGLIPEE FGVIARYKGQ GRLADPGFRN PRWVDGELVI LDGKYVKGGP
VVGFVYWAPE YHFVMFFNRL RLQA
