ID   EXEC2_ARATH             Reviewed;         651 AA.
AC   Q94AT5; Q9SXD0;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Protein EXECUTER 2, chloroplastic {ECO:0000305};
DE            Short=AtEX2 {ECO:0000303|PubMed:17540731};
DE   Flags: Precursor;
GN   Name=EX2 {ECO:0000303|PubMed:17540731};
GN   OrderedLocusNames=At1g27510 {ECO:0000312|Araport:AT1G27510};
GN   ORFNames=T17H3.1 {ECO:0000312|EMBL:AAD45989.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=17540731; DOI=10.1073/pnas.0702061104;
RA   Lee K.P., Kim C., Landgraf F., Apel K.;
RT   "EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from
RT   the plastid to the nucleus of Arabidopsis thaliana.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:10270-10275(2007).
RN   [5]
RP   FUNCTION.
RX   PubMed=22797473; DOI=10.1105/tpc.112.100479;
RA   Kim C., Meskauskiene R., Zhang S., Lee K.P., Lakshmanan Ashok M.,
RA   Blajecka K., Herrfurth C., Feussner I., Apel K.;
RT   "Chloroplasts of Arabidopsis are the source and a primary target of a
RT   plant-specific programmed cell death signaling pathway.";
RL   Plant Cell 24:3026-3039(2012).
CC   -!- FUNCTION: Together with EX1, enables higher plants to perceive singlet
CC       oxygen as a stress signal in plastid that activates a genetically
CC       determined nuclear stress response program which triggers a programmed
CC       cell death (PCD). This transfer of singlet oxygen-induced stress-
CC       related signals from the plastid to the nucleus that triggers
CC       genetically controlled PCD pathway is unique to photosynthetic
CC       eukaryotes and operates under mild stress conditions, impeding
CC       photosystem II (PSII) without causing photooxidative damage of the
CC       plant. {ECO:0000269|PubMed:17540731, ECO:0000269|PubMed:22797473}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:17540731}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD45989.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC005916; AAD45989.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE30840.1; -; Genomic_DNA.
DR   EMBL; AY045812; AAK76486.1; -; mRNA.
DR   EMBL; AY114085; AAM45133.1; -; mRNA.
DR   PIR; B86400; B86400.
DR   RefSeq; NP_564287.1; NM_102515.3.
DR   AlphaFoldDB; Q94AT5; -.
DR   SMR; Q94AT5; -.
DR   STRING; 3702.AT1G27510.1; -.
DR   PaxDb; Q94AT5; -.
DR   PRIDE; Q94AT5; -.
DR   ProteomicsDB; 222265; -.
DR   EnsemblPlants; AT1G27510.1; AT1G27510.1; AT1G27510.
DR   GeneID; 839642; -.
DR   Gramene; AT1G27510.1; AT1G27510.1; AT1G27510.
DR   KEGG; ath:AT1G27510; -.
DR   Araport; AT1G27510; -.
DR   TAIR; locus:2196889; AT1G27510.
DR   eggNOG; ENOG502QQS3; Eukaryota.
DR   HOGENOM; CLU_019201_0_0_1; -.
DR   InParanoid; Q94AT5; -.
DR   OMA; SSLEWDW; -.
DR   OrthoDB; 442207at2759; -.
DR   PhylomeDB; Q94AT5; -.
DR   PRO; PR:Q94AT5; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q94AT5; baseline and differential.
DR   Genevisible; Q94AT5; AT.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0042651; C:thylakoid membrane; IDA:TAIR.
DR   GO; GO:0000304; P:response to singlet oxygen; IBA:GO_Central.
DR   GO; GO:0010343; P:singlet oxygen-mediated programmed cell death; IGI:TAIR.
DR   InterPro; IPR044680; EX1/2.
DR   PANTHER; PTHR33917; PTHR33917; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Plastid; Reference proteome; Stress response; Transit peptide.
FT   TRANSIT         1..69
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           70..651
FT                   /note="Protein EXECUTER 2, chloroplastic"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000431895"
FT   DOMAIN          103..138
FT                   /note="UVR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00217"
FT   REGION          330..359
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   651 AA;  72131 MW;  79ABB2D52B0B3EED CRC64;
     MATTQPCLIG QIIAVPQFHI LFSPRNSLKP ELSTNKRTNF SVSIGLRHSF ASSISTCNPK
     APSLSCLRNC AAVDGADTSS SEDKWDWDWD RWNRHFSEIE EVESVVSLLK SQLEDAVEKE
     DFEEAVKLKQ AISEATVDDA VAEIMRQLQT AVNEERYHDA SRLCNETGSG LVGWWVGLPR
     DSEEPFGRIV HITPGVGRFI GKSYSPRQLV AEAAGTPLFE IFVIKDTDGG YVMQVVYVQH
     VKQNLTISEN SFSKVQQSSK SSINDPSILD VRGSELKVDK KEDTQLNAGE PTEEGIKNVI
     KFLKDKIPGL KLKVMDVIKI PEEEIVGSDD ATEELVGEGT EETNSSDDEE EVEEEENDSI
     EAISSMDSAD YGKHSNTKLV IGGVLHNIED SSIDDEIVRV SANIMDTERD SFILHVPGRS
     KRDIDTRKNR VSKEQVTALA AQGLSDLLPP EVAEAFWGEK ASLKVSKHVH EIVKLAINQA
     QKGNHLSEYT AFNRIITPES NLDPFDGLYV GAFGPYGTEI VQLKRKYGRW DDAEGSNSSD
     IEFFEYVEAV KLTGDPNVPA GQVTFRARIG NGSRMTNHGL FPEELGVLAS YRGQGKIADF
     GFKKPRWVEG KLLKLNGKGM GPYVKGADLG FLYIGPEQSF LVLFNRLRLP E